Cell No. : Cell Name
RCB0197 : HUC-Fm
update : 2024/08/29
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Comment | Normal umbilical cord fibroblast, male |
Comment from the depositor | |
Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
Remarks | |
Order Form |
Order Form(C-0005.pdf)
 
MTA(C-0007.pdf)
 
MTA(C-0007p.pdf)
 
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
Ohno,T. & Nishida,M.
|
Originator |
Ohno, Tadao
|
Year of deposit |
1987
|
Animal |
_human
< Mammals
|
Genus |
Homo
|
Species |
sapiens
|
Race |
Japanese
|
Gender |
Male
|
Tissue |
umbilical cord
|
Classification |
normal
|
Year of origin |
1987
|
|
Lifespan |
finite
|
Morphology |
fibroblast-like
|
Cellosaurus(Expasy) |
CVCL_2524
|
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deposit info |
lot info |
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Medium |
Medium List |
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Culture type |
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Adherent cells
|
Culture medium |
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HF-RITC80-7 + 10% FBS
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Antibiotics |
|
Free
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|
Passage method |
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0.25% Trypsin
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Culture information
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Passage ratio |
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1 : 4 split
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SC frequency |
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Subculture : 2 times/week
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Temperature |
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37
℃
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CO2 concentration |
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5
%
|
Freeze medium |
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Medium + 10% DMSO
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Freezing method |
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Slow freezing
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Mycoplasma |
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(-)
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Isozyme |
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LD, NP
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Chromosome mode |
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43-46(50) : 43(1),44(1),45(5),46(43)
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STR(human) |
|
OK
|
Images |
deposit info | lot info |
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Reference information |
Reference |
1
|
User's Publication |
5
|
User's Publication |
6028
Kawamura F et al
Establishment of induced pluripotent stem cells from normal B cells and inducing AID expression in their differentiation into hematopoietic progenitor cells.
Sci Rep
2017
7(1):1659
PubMed ID: 28490810
DOI: 10.1038/s41598-017-01627-1
|
9109
Fujioka T, Shimizu N, Yoshino K, Miyoshi H, Nakamura Y.
Establishment of induced pluripotent stem cells from human neonatal tissues.
Hum Cell
2010
23(3):113-8
PubMed ID: 20973836
DOI: 10.1111/j.1749-0774.2010.00091.x
|
8788
Hiroyama T, Sudo K, Aoki N, Miharada K, Danjo I, Fujioka T, Nagasawa T, Nakamura Y.
Human umbilical cord-derived cells can often serve as feeder cells to maintain primate embryonic stem cells in a state capable of producing hematopoietic cells.
Cell Biol. Int.
2008
32:1-7
PubMed ID: 17890111
DOI: 10.1016/j.cellbi.2007.08.001
|
9428
Chirila T, Barnard Z, Zainuddin, Harkin DG, Schwab IR, Hirst L.
Bombyx mori silk fibroin membranes as potential substrata for epithelial constructs used in the management of ocular surface disorders.
Tissue Eng Part A
2008
14(7):1203-11
PubMed ID: 18380593
DOI: 10.1089/ten.tea.2007.0224
|
5545
Higuchi A, Yoshida M, Ohno T, Asakura T, Hara M.
Production of interferon-beta in a culture of fibroblast cells on some polymeric films.
Cytotechnology
2000
34(1-2):165-73
PubMed ID: 19003391
DOI: 10.1023/A:1008130223190
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