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Cell No. : Cell Name
RCB0197 : HUC-Fm  update : 2022/03/07
CommentNormal umbilical cord fibroblast, male
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Ohno,T. & Nishida,M.
Originator Ohno, Tadao
Year of deposit 1987
Animal _human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Male
Tissue umbilical cord
Classification normal
Year of origin 1987
Lifespan finite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_2524
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium HF-RITC80-7 + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 4 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Isozyme LD, NP
Chromosome mode 43-46(50) : 43(1),44(1),45(5),46(43)
STR(human) OK
deposit info
lot info
Reference information Reference 1
User's Publication 5

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893  Sudo K, Kanno M, Miharada K, Ogawa S, Hiroyama T, Saijo K, Nakamura Y.  Mesenchymal progenitors able to differentiate into osteogenic, chondrogenic, and/or adipogenic cells in vitro are present in most primary fibroblast-like cell populations  Stem Cells  2007  25(7):1610-7  PubMed ID: 17395773   DOI: 10.1634/stemcells.2006-0504

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User's Publication
6028  Kawamura F et al  Establishment of induced pluripotent stem cells from normal B cells and inducing AID expression in their differentiation into hematopoietic progenitor cells.  Sci Rep  2017  7(1):1659  PubMed ID: 28490810   DOI: 10.1038/s41598-017-01627-1
9109  Fujioka T, Shimizu N, Yoshino K, Miyoshi H, Nakamura Y.  Establishment of induced pluripotent stem cells from human neonatal tissues.  Hum Cell  2010  23(3):113-8  PubMed ID: 20973836   DOI: 10.1111/j.1749-0774.2010.00091.x
8788  Hiroyama T, Sudo K, Aoki N, Miharada K, Danjo I, Fujioka T, Nagasawa T, Nakamura Y.  Human umbilical cord-derived cells can often serve as feeder cells to maintain primate embryonic stem cells in a state capable of producing hematopoietic cells.  Cell Biol. Int.  2008  32:1-7  PubMed ID: 17890111   DOI: 10.1016/j.cellbi.2007.08.001
9428  Chirila T, Barnard Z, Zainuddin, Harkin DG, Schwab IR, Hirst L.  Bombyx mori silk fibroin membranes as potential substrata for epithelial constructs used in the management of ocular surface disorders.  Tissue Eng Part A  2008  14(7):1203-11  PubMed ID: 18380593   DOI: 10.1089/ten.tea.2007.0224
5545  Higuchi A, Yoshida M, Ohno T, Asakura T, Hara M.  Production of interferon-beta in a culture of fibroblast cells on some polymeric films.  Cytotechnology  2000  34(1-2):165-73  PubMed ID: 19003391   DOI: 10.1023/A:1008130223190

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