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Cell No. : Cell Name
RCB0195 : PAb419  update : 2022/03/07
CommentAnti-SV40 large T antigen mAb.
Comment from the depositor
Terms and conditionsA prior written permission of the approver(Depositor) is necessary for any kind of use including academic use and for-profit use.
Remarks
approver's address
×
English
Address
Tokyo University of Science

2641 Yamazaki, Noda-shi, Chiba-ken 278-8510 Japan
Dr.MURAKAMI Yasufumi
Fax. +81-4-7122-1360
Order Form Order Form(C-0005.pdf)   Approval Form(C-0006.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Murakami, Yasufumi
Year of deposit 1987
Animal mouse x mouse < Hybrid
Tissue hybridoma
Classification hybrid cells
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_B954
hybridoma Fusion_cell_1 strain mouse
Fusion_cell_2 strain mouse
Subclass IgG1
Antigen_name SV40 T antigen
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives GIT
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 10 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium GIT : 2 x FM1 = 1 : 1
Freezing method Slow freezing
Mycoplasma (-)
Antibody subclass IgG2a
Images
deposit info
lot info
Reference information Reference 3
User's Publication 1


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Reference
4617  Stedman D, Whittaker L, Hand R.  Simian virus 40 large T antigen oligomers: analysis of electrophoresis in the absence of detergent.  J Virol  1985  56(3):711-6  PubMed ID: 2999426  
4616  Crawford L, Leppard K, Lane D, Harlow E.  Cellular proteins reactive with monoclonal antibodies directed against simian virus 40 T-antigen.  J Virol  1982  42(2):612-20  PubMed ID: 6177872  
4615  Harlow E, Crawford LV, Pim DC, Williamson NM.  Monoclonal antibodies specific for simian virus 40 tumor antigens.  J Virol  1981  39(3):861-9  PubMed ID: 6169844  

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User's Publication
15585  Miyata Y, Yahara I.  p53-independent association between SV40 large T antigen and the major cytosolic heat shock protein, HSP90  Oncogene  2000  19(11):1477-84  PubMed ID: 10723140   DOI: 10.1038/sj.onc.1203475



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