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Cell No. : Cell Name
RCB0193 : KMM-1  update : 2022/11/04
CommentHuman myeloma. Lambda- chain producing.
Comment from the depositor
Terms and conditionsA prior written permission of the approver(Depositor) is necessary for any kind of use including academic use and for-profit use.
approver's address
Okayama University Graduate School of Medicine
Dentistry, and Pharmaceutical Sciences, Department of Cell Biology
2-5-1 shikata-cho , Kita-ku , Okayama-shi, Okayama 700-8558 Japan
Fax. +81-86-235-7400
Order Form Order Form(C-0005.pdf)   Approval Form(C-0006.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Huh, Nam, Ho
Originator Namba, Masayoshi
Year of deposit 1987
Animal _human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Male
Age at sampling 62 years
Tissue myeloma, subcutaneous
Disease name multiple myeloma
Metastastatic ability Yes
Metastatsd tissue Multiple
Classification cancer
Year of origin 1980
History Namba,M.->RCB
Memo_1 Mycoplasma was eliminated with MC-210.
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_2981
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Culture medium RPMI1640 + 10% FBS
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 10 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Virus (HIV) (-)
Isozyme LD, NP
Chromosome mode 38-166(50) : /77(4),78(4),79(4),80(5),81(4),82(3)/
STR(human) OK
Reference information Reference 10
User's Publication 1

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4627  Bai L, Naomoto Y, Miyazaki M, Orita K, Namba M.  Antiproliferative effects of suramin on human cancer cells in vitro and in vivo.  Acta Med Okayama  1992  46(6):457-63  PubMed ID: 1485540   DOI: 10.18926/AMO/32642
4653  Namba M, Ohtsuki T, Mori M, Togawa A, Wada H, Sugihara T, Yawata Y, Kimoto T.  Establishment of five human myeloma cell lines.  In Vitro Cell Dev Biol  1989  25(8):723-9  PubMed ID: 2768132   DOI: 10.1007/BF02623725
4654  Ohtsuki T, Yawata Y, Namba M.  Establishment and characterization of five human myeloma cell lines  Hum Cell  1989  2(3):297-303  PubMed ID: 2519219  
4652  Ohtsuki T, Yawata Y, Namba M.  Cell growth kinetics of cultured human myeloma cell lines, KMM-1 and KMS-5.  Nihon Ketsueki Gakkai Zasshi  1988  51(6):1052-8  PubMed ID: 3201898  
4650  Miyoshi T, Ogawa S, Kanamori T, Nobuhara M, Namba M.  Interferon potentiates cytotoxic effects of 5-fluorouracil on cell proliferation of established human cell lines originating from neoplastic tissues.  Cancer Lett  1983  17(3):239-47  PubMed ID: 6187435   DOI: 10.1016/0304-3835(83)90160-x
4651  Togawa A, Inoue N, Mannoji M, Namba M.  B-cell differentiation antigen expressed on a human myeloma cell line (KMM-1).  Jpn J Clin Oncol  1983  13(3):511-5  PubMed ID: 6417374  
1024  Togawa A, Inoue N, Miyamoto K, Hyodo H, Namba M  Establishment and characterization of a human myeloma cell line (KMM-1).  Int J Cancer  1982  29:495-500  PubMed ID: 7095894   DOI: 10.1002/ijc.2910290502
4647  Togawa A, Inoue N, Shimoda M, Mannoji M, Yawata Y, Miyamoto K, Namba M.  Establishment and characterization of a human myeloma cell line (KMM-1)  Nihon Ketsueki Gakkai Zasshi  1982  45(3):607-14  PubMed ID: 7136460  
4648  Namba M, Miyoshi T, Kanamori T, Nobuhara M, Kimoto T, Ogawa S.  Combined effects of 5-fluorouracil and interferon on proliferation of human neoplastic cells in culture.  Gan  1982  73(5):819-24  PubMed ID: 6187620  
4649  Miyoshi T, Ogawa S, Kanamori T, Nobuhara M, Kimoto T, Namba M.  Combined effects of 5-fluorouracil and interferon on proliferation of human neoplastic cells in culture  Gan To Kagaku Ryoho  1982  9(12):2207-11  PubMed ID: 6191694  

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User's Publication
14309  Ninomiya S, Kawano M, Abe T, Ishikawa T, Takahashi M, Tamura M, Takahashi Y, Nashimoto M.  Potential small guide RNAs for tRNase ZL from human plasma, peripheral blood mononuclear cells, and cultured cell lines  PLoS One  2015  10(3):e0118631  PubMed ID: 25730316   DOI: 10.1371/journal.pone.0118631

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