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細胞番号 : 細胞名
RCB0191 : HeLa.S3  update : 2018/07/06
細胞特性(Comment:英)Most famous cultured human cell line
使用条件(英)Basically, there is no restriction regarding academic use.
提供申込書類 依頼書C-0001.pdf   同意書(非営利学術目的)C-0003.pdf   同意書(営利目的)C-0003p.pdf  
提供手数料 手数料とお支払いについてはこちらをご覧ください。
細胞基本情報 寄託者 Murakami, Yasufumi
寄託日 1987
動物種 human < Mammals
属名 Homo
種名 sapiens
性別 Female
採取組織 cervix
病歴 cervical carcinoma
細胞分類 cancer
細胞寿命 infinite
細胞形態 other
培地・試薬情報 培地・試薬一覧はこちらをご覧ください。
培養形態 Suspension cells
培地 MEM (suspension, 2mM L-Glutamine) + 10% CS
抗生物質 Free
継代方法 dilution
継代密度 1 : 8 split
継代・培地交換頻度 Subculture : once/week, Medium Renewal : 2 times/week
培養最適温度 37 ℃
二酸化炭素濃度 5 %
凍結培地 Medium + 10% DMSO
凍結方法 Slow freezing
マイコプラズマ (-)
個体識別検査 OK
ウィルス(HIV) (-)
アイソザイム検査 LD, NP
染色体検査 56-271(50) : /64(2),65(2),66(6),67(20),68(8),69(2)/
文献情報 Reference(英) 5件
Reference(日) 2件
利用者成果(英) 15件
利用者成果(日) 2件

207  Prescott,D.M. (ed.)    Methods Cell Biol  1973  4:1-42 
350  Kuroki T.  Colony formation of mammalian cells on agar plates and its application to Lederberg's replica plating.  Exp Cell Res  1973  80(1):55-62  PubMed ID: 4361348
1445  SATO G, FISHER HW, PUCK TT.  Molecular growth requirements of single mammalian cells.  Science  1957  126(3280):461-4  PubMed ID: 13486039
206  PUCK TT, MARCUS PI, CIECIURA SJ.  Clonal growth of mammalian cells in vitro; growth characteristics of colonies from single HeLa cells with and without a feeder layer.  J Exp Med  1956  103(2):273-83  PubMed ID: 13286432
150  Gey,G.O., Coffman,W.D. and Kubicek,M.T.  Tissue culture studies of the proliferative capacity of cervical carcinoma and normal epithelium.  Cancer Res  1952  12:264-265 

4614  梅田 誠, 松村 外志張, 中家 茂  培養液の検索法について~HeLa S3細胞による方法~  組織培養研究  1991  10:71-76 
1228  花岡文雄  バイオテクノロジ-素材としての培養細胞 体細胞遺伝学  蛋白質 核酸 酵素  1988  33:291-292 

12005  Kabayama H, Takeuchi M, Tokushige N, Muramatsu SI, Kabayama M, Fukuda M, Yamada Y, Mikoshiba K.  An ultra-stable cytoplasmic antibody engineered for in vivo applications.  Nat Commun  2020    PubMed ID: 31953402
4482  Roppongi M, Izumisawa M, Terasaki K, Muraki Y, Shozushima M.  18F-FDG and 11C-choline uptake in proliferating tumor cells is dependent on the cell cycle in vitro.  Ann Nucl Med  2019  33(4):237-243  PubMed ID: 30588580
6049  Sawada JI, Ishii H, Matsuno K, Sato M, Suzuki Y, Asai A.  Selective Inhibition of Spindle Microtubules by a Tubulin-Binding Quinazoline Derivative.  Mol Pharmacol  2019  96(5):609-618  PubMed ID: 31471455
10289  Murakami K, Zhao J, Yamasaki K, Miyagishi M.  Biochemical and structural features of extracellular vesicle-binding RNA aptamers.  Biomed Rep  2017  6:615-626  PubMed ID: 28584632
10026  Ojima D, Yasui A, Tohyama K, Tokuzumi K, Toriihara E, Ito K, Iwasaki A, Tomura T, Ojika M, Suenaga K.  Total Synthesis of Miuraenamides A and D.  J. Org. Chem.  2016  81:9886-9894  PubMed ID: 27662058
10366  Makoto Kimura  IRF2-binding protein-1 Is a JDP2 Ubiquitin Ligase and an Inhibitor of ATF2-dependent Transcription  FEBS Lett  2008  582(19):2833-7  PubMed ID: 18671972
8795  Engelhardt P, Merilainen J, Zhao F, Uchiyama S, Fukui K, Lehto VP.  Whole-mount immunoelectron tomography of chromosomes and cells.  Methods Mol. Biol.  2007  369:387-405  PubMed ID: 17656761
5595  Nakanishi A, Han X, Saito H, Taguchi K, Ohta Y, Imajoh-Ohmi S, Miki Y.  Interference with BRCA2, which localizes to the centrosome during S and early M phase, leads to abnormal nuclear division.  Biochem Biophys Res Commun  2007  355(1):34-40  PubMed ID: 17286961
3097  Uchiyama S, Kobayashi S, Takata H, Ishihara T, Hori N, Higashi T, Hayashihara K, Sone T, Higo D, Nirasawa T, Takao T, Matsunaga S, Fukui K.  Proteome analysis of human metaphase chromosomes.  J Biol Chem  2005  280(17):16994-7004  PubMed ID: 15687487
6278  Masanori SHOZUSHIMA, Reiko TSUTSUMI, Shigehiro SATO, Kazunori TERASAKI, Mitsuru IZUMISAWA, Hitoshi SATO, Satoru SHOJI, Kimio SAKAMAKI  Quantification of 11C-methionine Uptake During Proliferation of Cultured Human Cancer Cells  RADIOISOTOPES  2003  52:456-463 
5010  Shimabukuro K, Ichinose S, Koike R, Kubota T, Yamaguchi M, Miyasaka M, Aso T.  Hepatocyte growth factor/scatter factor is implicated in the mode of stromal invasion of uterine squamous cervical cancer.  Gynecol Oncol  2001  83(2):205-15  PubMed ID: 11606073
2531  Masters JR, Thomson JA, Daly-Burns B, Reid YA, Dirks WG, Packer P, Toji LH, Ohno T, Tanabe H, Arlett CF, Kelland LR, Harrison M, Virmani A, Ward TH, Ayres KL, Debenham PG.  Short tandem repeat profiling provides an international reference standard for human cell lines.  Proc Natl Acad Sci U S A.  2001  98(14):8012-7  PubMed ID: 11416159
3078  Takahashi S, Takahashi Y, Ito K, Nagano T, Shibahara S, Miura T.  Positive and negative regulation of the human heme oxygenase-1 gene expression in cultured cells.  Biochim Biophys Acta  1999  1447(2-3):231-5  PubMed ID: 10542320
3753  Murakami K, Nomiyama H, Miura R, Follens A, Fiten P, Van Coillie E, Van Damme J, Opdenakker G.  Structural and functional analysis of the promoter region of the human MCP-3 gene: transactivation of expression by novel recognition sequences adjacent to the transcription initiation site.  DNA Cell Biol  1997  16(2):173-83  PubMed ID: 9052738
1945  Kobayashi S, Satoh H, Takehana M.  Protective effects of glutathione isopropyl ester on the sensitivity of cultured cells to UVB irradiation.  Biol Pharm Bull  1995  18(9):1219-22  PubMed ID: 8845809

8781  小豆島正典、山本純子、原 康文、寺崎一典、後藤祥子、岩田 錬  細胞周期に対する18F-Cholineと18FDG集積の比較  NMCC共同利用研究成果報文集  2008  15:231-235 
8865  小豆島正典、山本純子、原 康文、寺崎一典、後藤祥子、岩田錬  HeLa 細胞に対する 18F-Choline 集積の細胞周期依存性  NMCC共同利用研究成果報文集  2006  14:256-287 

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