細胞番号 : 細胞名
RCB0191 : HeLa.S3
update : 2024/08/14
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| 細胞特性(Comment:英) | Most famous cultured human cell line |
| 細胞特性(日) | HeLaの亜株の一つでコロニー形成率が非常に高いことで知られる。この細胞番号のHeLa.S3は、浮遊状態（スピナーフラスコ等）での培養も可能である。 |
| 細胞特性(寄託者記述:英) | |
| 細胞特性(寄託者記述:日) | |
| 使用条件(英) | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| 使用条件(日) | 基礎的研究につきましては、使用制限はありません。商業利用や特許取得を目的とする場合は、事前に必ず理研細胞バンクに連絡をすること。 |
| 備考(英) | |
| 備考(日) | |
| 提供申込書類(英) |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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| 提供申込書類(日) |
依頼書C-0001.pdf
同意書(非営利学術目的)C-0003.pdf
同意書(営利目的)C-0003p.pdf
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提供同意書は、使用機関の種類や目的に応じて、非営利学術目的 (C-XXXX) と営利目的 (C-XXXXp) の2種類があります。該当する提供同意書をご使用ください（詳細）。特許等の取得及び商業利用等は事前に必ず cellbank.brc@riken.jp までご連絡ください。
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| 提供手数料 |
手数料とお支払いについてはこちらをご覧ください。 |
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細胞基本情報
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寄託者 |
Murakami, Yasufumi
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| 寄託日 |
1987
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| 動物種 |
_human
< Mammals
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| 属名 |
Homo
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| 種名 |
sapiens
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| 性別 |
Female
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| 採取組織 |
cervix
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| 病名 |
cervical carcinoma
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| 細胞分類 |
cancer
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|
細胞寿命 |
infinite
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| 細胞形態 |
other
|
| Cellosaurus(Expasy) |
CVCL_0058
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細胞培養・検査情報
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寄託時情報 |
ロット情報 |
| 培地・試薬情報 |
培地・試薬一覧はこちらをご覧ください。 |
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培養形態 |
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Suspension cells
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| 培養方法 |
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浮遊細胞の培養に関する一般的な注意(Japanese)
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| 培地 |
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MEM (suspension, 2mM L-Glutamine) + 10% CS
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| 抗生物質 |
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Free
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継代方法 |
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dilution
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|
継代密度 |
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1 : 8 split
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| 継代・培地交換頻度 |
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Subculture : once/week, Medium Renewal : 2 times/week
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| 培養最適温度 |
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37
℃
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| 二酸化炭素濃度 |
|
5
%
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| 凍結培地 |
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Medium + 10% DMSO
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| 凍結方法 |
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Slow freezing
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| マイコプラズマ/アコレプラズマ |
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(-)
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| ウィルス（HIV) |
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(-)
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| アイソザイム検査 |
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LD, NP
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| 染色体検査 |
|
56-271(50) : /64(2),65(2),66(6),67(20),68(8),69(2)/
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| 個体識別検査 |
|
OK
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| 文献情報 |
Reference(英) |
6件
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| Reference(日) |
2件
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| 利用者成果(英) |
20件
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| 利用者成果(日) |
2件
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| 利用者成果(英) |
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21643
Apostolopoulos A, Kawamoto N, Chow SYA, Tsuiji H, Ikeuchi Y, Shichino Y, Iwasaki S.
dCas13-mediated translational repression for accurate gene silencing in mammalian cells.
Nat Commun
2024
15(1):2205
PubMed ID: 38467613
DOI: 10.1038/s41467-024-46412-7
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19978
Hashizume T, Ozawa Y, Ying BW.
Employing active learning in the optimization of culture medium for mammalian cells
NPJ Syst Biol Appl
2023
9(1):20
PubMed ID: 37253825
DOI: 10.1038/s41540-023-00284-7
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16700
Sawada JI, Matsuno K, Ogo N, Asai A.
Various effects of two types of kinesin-5 inhibitors on mitosis and cell proliferation
Biochem Pharmacol
2021
193:114789
PubMed ID: 34582773
DOI: 10.1016/j.bcp.2021.114789
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12005
Kabayama H, Takeuchi M, Tokushige N, Muramatsu SI, Kabayama M, Fukuda M, Yamada Y, Mikoshiba K.
An ultra-stable cytoplasmic antibody engineered for in vivo applications.
Nat Commun
2020
PubMed ID: 31953402
DOI: 10.1038/s41467-019-13654-9
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4482
Roppongi M, Izumisawa M, Terasaki K, Muraki Y, Shozushima M.
18F-FDG and 11C-choline uptake in proliferating tumor cells is dependent on the cell cycle in vitro.
Ann Nucl Med
2019
33(4):237-243
PubMed ID: 30588580
DOI: 10.1007/s12149-018-01325-6
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6049
Sawada JI, Ishii H, Matsuno K, Sato M, Suzuki Y, Asai A.
Selective Inhibition of Spindle Microtubules by a Tubulin-Binding Quinazoline Derivative.
Mol Pharmacol
2019
96(5):609-618
PubMed ID: 31471455
DOI: 10.1124/mol.119.116624
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10289
Murakami K, Zhao J, Yamasaki K, Miyagishi M.
Biochemical and structural features of extracellular vesicle-binding RNA aptamers.
Biomed Rep
2017
6:615-626
PubMed ID: 28584632
DOI: 10.3892/br.2017.899
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10026
Ojima D, Yasui A, Tohyama K, Tokuzumi K, Toriihara E, Ito K, Iwasaki A, Tomura T, Ojika M, Suenaga K.
Total Synthesis of Miuraenamides A and D.
J. Org. Chem.
2016
81:9886-9894
PubMed ID: 27662058
DOI: 10.1021/acs.joc.6b02061
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15196
Aizawa M, Fukuda M.
Small GTPase Rab2B and Its Specific Binding Protein Golgi-associated Rab2B Interactor-like 4 (GARI-L4) Regulate Golgi Morphology
J Biol Chem
2015
290(36):22250-61
PubMed ID: 26209634
DOI: 10.1074/jbc.M115.669242
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10366
Makoto Kimura
IRF2-binding protein-1 Is a JDP2 Ubiquitin Ligase and an Inhibitor of ATF2-dependent Transcription
FEBS Lett
2008
582(19):2833-7
PubMed ID: 18671972
DOI: 10.1016/j.febslet.2008.07.033
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8795
Engelhardt P, Merilainen J, Zhao F, Uchiyama S, Fukui K, Lehto VP.
Whole-mount immunoelectron tomography of chromosomes and cells.
Methods Mol. Biol.
2007
369:387-405
PubMed ID: 17656761
DOI: 10.1007/978-1-59745-294-6_19
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5595
Nakanishi A, Han X, Saito H, Taguchi K, Ohta Y, Imajoh-Ohmi S, Miki Y.
Interference with BRCA2, which localizes to the centrosome during S and early M phase, leads to abnormal nuclear division.
Biochem Biophys Res Commun
2007
355(1):34-40
PubMed ID: 17286961
DOI: 10.1016/j.bbrc.2007.01.100
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3097
Uchiyama S, Kobayashi S, Takata H, Ishihara T, Hori N, Higashi T, Hayashihara K, Sone T, Higo D, Nirasawa T, Takao T, Matsunaga S, Fukui K.
Proteome analysis of human metaphase chromosomes.
J Biol Chem
2005
280(17):16994-7004
PubMed ID: 15687487
DOI: 10.1074/jbc.M412774200
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6278
Masanori SHOZUSHIMA, Reiko TSUTSUMI, Shigehiro SATO, Kazunori TERASAKI, Mitsuru IZUMISAWA, Hitoshi SATO, Satoru SHOJI, Kimio SAKAMAKI
Quantification of 11C-methionine Uptake During Proliferation of Cultured Human Cancer Cells
RADIOISOTOPES
2003
52:456-463
DOI: 10.3769/radioisotopes.52.456
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5010
Shimabukuro K, Ichinose S, Koike R, Kubota T, Yamaguchi M, Miyasaka M, Aso T.
Hepatocyte growth factor/scatter factor is implicated in the mode of stromal invasion of uterine squamous cervical cancer.
Gynecol Oncol
2001
83(2):205-15
PubMed ID: 11606073
DOI: 10.1006/gyno.2001.6347
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2531
Masters JR, Thomson JA, Daly-Burns B, Reid YA, Dirks WG, Packer P, Toji LH, Ohno T, Tanabe H, Arlett CF, Kelland LR, Harrison M, Virmani A, Ward TH, Ayres KL, Debenham PG.
Short tandem repeat profiling provides an international reference standard for human cell lines.
Proc Natl Acad Sci U S A.
2001
98(14):8012-7
PubMed ID: 11416159
DOI: 10.1073/pnas.121616198
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3078
Takahashi S, Takahashi Y, Ito K, Nagano T, Shibahara S, Miura T.
Positive and negative regulation of the human heme oxygenase-1 gene expression in cultured cells.
Biochim Biophys Acta
1999
1447(2-3):231-5
PubMed ID: 10542320
DOI: 10.1016/s0167-4781(99)00156-6
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15093
Kitagawa M, Mukai H, Takahashi M, Ono Y.
The role of PKN in the regulation of alphaB-crystallin expression via heat shock transcription factor 1
Biochem Biophys Res Commun
1998
252(3):561-5
PubMed ID: 9837746
DOI: 10.1006/bbrc.1998.9694
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3753
Murakami K, Nomiyama H, Miura R, Follens A, Fiten P, Van Coillie E, Van Damme J, Opdenakker G.
Structural and functional analysis of the promoter region of the human MCP-3 gene: transactivation of expression by novel recognition sequences adjacent to the transcription initiation site.
DNA Cell Biol
1997
16(2):173-83
PubMed ID: 9052738
DOI: 10.1089/dna.1997.16.173
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1945
Kobayashi S, Satoh H, Takehana M.
Protective effects of glutathione isopropyl ester on the sensitivity of cultured cells to UVB irradiation.
Biol Pharm Bull
1995
18(9):1219-22
PubMed ID: 8845809
DOI: 10.1248/bpb.18.1219
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