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Cell No. : Cell Name
RCB0191 : HeLa.S3  update : 2024/05/14
CommentMost famous cultured human cell line
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Murakami, Yasufumi
Year of deposit 1987
Animal _human < Mammals
Genus Homo
Species sapiens
Gender Female
Tissue cervix
Disease name cervical carcinoma
Classification cancer
Lifespan infinite
Morphology other
Cellosaurus(Expasy) CVCL_0058
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Culture medium MEM (suspension, 2mM L-Glutamine) + 10% CS
Culture method
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Virus (HIV) (-)
Isozyme LD, NP
Chromosome mode 56-271(50) : /64(2),65(2),66(6),67(20),68(8),69(2)/
STR(human) OK
Reference information Reference 6
User's Publication 20

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207  Prescott,D.M. (ed.)    Methods Cell Biol  1973  4:1-42   
350  Kuroki T.  Colony formation of mammalian cells on agar plates and its application to Lederberg's replica plating.  Exp Cell Res  1973  80(1):55-62  PubMed ID: 4361348   DOI: 10.1016/0014-4827(73)90274-7
1446  J E DARNELL Jr, T K SAWYERc  Variation in plaque-forming ability among parental and clonal strains of HeLa cells  Virology  1959  223-9  PubMed ID: 13669339   DOI: 10.1016/0042-6822(59)90006-6
1445  SATO G, FISHER HW, PUCK TT.  Molecular growth requirements of single mammalian cells.  Science  1957  126(3280):461-4  PubMed ID: 13486039  
206  PUCK TT, MARCUS PI, CIECIURA SJ.  Clonal growth of mammalian cells in vitro; growth characteristics of colonies from single HeLa cells with and without a feeder layer.  J Exp Med  1956  103(2):273-83  PubMed ID: 13286432   DOI: 10.1084/jem.103.2.273
150  Gey,G.O., Coffman,W.D. and Kubicek,M.T.  Tissue culture studies of the proliferative capacity of cervical carcinoma and normal epithelium.  Cancer Res  1952  12:264-265   

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User's Publication
21643  Apostolopoulos A, Kawamoto N, Chow SYA, Tsuiji H, Ikeuchi Y, Shichino Y, Iwasaki S.  dCas13-mediated translational repression for accurate gene silencing in mammalian cells.  Nat Commun  2024  15(1):2205  PubMed ID: 38467613   DOI: 10.1038/s41467-024-46412-7
19978  Hashizume T, Ozawa Y, Ying BW.  Employing active learning in the optimization of culture medium for mammalian cells  NPJ Syst Biol Appl  2023  9(1):20  PubMed ID: 37253825   DOI: 10.1038/s41540-023-00284-7
16700  Sawada JI, Matsuno K, Ogo N, Asai A.  Various effects of two types of kinesin-5 inhibitors on mitosis and cell proliferation  Biochem Pharmacol  2021  193:114789  PubMed ID: 34582773   DOI: 10.1016/j.bcp.2021.114789
12005  Kabayama H, Takeuchi M, Tokushige N, Muramatsu SI, Kabayama M, Fukuda M, Yamada Y, Mikoshiba K.  An ultra-stable cytoplasmic antibody engineered for in vivo applications.  Nat Commun  2020    PubMed ID: 31953402   DOI: 10.1038/s41467-019-13654-9
4482  Roppongi M, Izumisawa M, Terasaki K, Muraki Y, Shozushima M.  18F-FDG and 11C-choline uptake in proliferating tumor cells is dependent on the cell cycle in vitro.  Ann Nucl Med  2019  33(4):237-243  PubMed ID: 30588580   DOI: 10.1007/s12149-018-01325-6
6049  Sawada JI, Ishii H, Matsuno K, Sato M, Suzuki Y, Asai A.  Selective Inhibition of Spindle Microtubules by a Tubulin-Binding Quinazoline Derivative.  Mol Pharmacol  2019  96(5):609-618  PubMed ID: 31471455   DOI: 10.1124/mol.119.116624
10289  Murakami K, Zhao J, Yamasaki K, Miyagishi M.  Biochemical and structural features of extracellular vesicle-binding RNA aptamers.  Biomed Rep  2017  6:615-626  PubMed ID: 28584632   DOI: 10.3892/br.2017.899
10026  Ojima D, Yasui A, Tohyama K, Tokuzumi K, Toriihara E, Ito K, Iwasaki A, Tomura T, Ojika M, Suenaga K.  Total Synthesis of Miuraenamides A and D.  J. Org. Chem.  2016  81:9886-9894  PubMed ID: 27662058   DOI: 10.1021/acs.joc.6b02061
15196  Aizawa M, Fukuda M.  Small GTPase Rab2B and Its Specific Binding Protein Golgi-associated Rab2B Interactor-like 4 (GARI-L4) Regulate Golgi Morphology  J Biol Chem   2015  290(36):22250-61  PubMed ID: 26209634   DOI: 10.1074/jbc.M115.669242
10366  Makoto Kimura  IRF2-binding protein-1 Is a JDP2 Ubiquitin Ligase and an Inhibitor of ATF2-dependent Transcription  FEBS Lett  2008  582(19):2833-7  PubMed ID: 18671972   DOI: 10.1016/j.febslet.2008.07.033
8795  Engelhardt P, Merilainen J, Zhao F, Uchiyama S, Fukui K, Lehto VP.  Whole-mount immunoelectron tomography of chromosomes and cells.  Methods Mol. Biol.  2007  369:387-405  PubMed ID: 17656761   DOI: 10.1007/978-1-59745-294-6_19
5595  Nakanishi A, Han X, Saito H, Taguchi K, Ohta Y, Imajoh-Ohmi S, Miki Y.  Interference with BRCA2, which localizes to the centrosome during S and early M phase, leads to abnormal nuclear division.  Biochem Biophys Res Commun  2007  355(1):34-40  PubMed ID: 17286961   DOI: 10.1016/j.bbrc.2007.01.100
3097  Uchiyama S, Kobayashi S, Takata H, Ishihara T, Hori N, Higashi T, Hayashihara K, Sone T, Higo D, Nirasawa T, Takao T, Matsunaga S, Fukui K.  Proteome analysis of human metaphase chromosomes.  J Biol Chem  2005  280(17):16994-7004  PubMed ID: 15687487   DOI: 10.1074/jbc.M412774200
6278  Masanori SHOZUSHIMA, Reiko TSUTSUMI, Shigehiro SATO, Kazunori TERASAKI, Mitsuru IZUMISAWA, Hitoshi SATO, Satoru SHOJI, Kimio SAKAMAKI  Quantification of 11C-methionine Uptake During Proliferation of Cultured Human Cancer Cells  RADIOISOTOPES  2003  52:456-463    DOI: 10.3769/radioisotopes.52.456
5010  Shimabukuro K, Ichinose S, Koike R, Kubota T, Yamaguchi M, Miyasaka M, Aso T.  Hepatocyte growth factor/scatter factor is implicated in the mode of stromal invasion of uterine squamous cervical cancer.  Gynecol Oncol  2001  83(2):205-15  PubMed ID: 11606073   DOI: 10.1006/gyno.2001.6347
2531  Masters JR, Thomson JA, Daly-Burns B, Reid YA, Dirks WG, Packer P, Toji LH, Ohno T, Tanabe H, Arlett CF, Kelland LR, Harrison M, Virmani A, Ward TH, Ayres KL, Debenham PG.  Short tandem repeat profiling provides an international reference standard for human cell lines.  Proc Natl Acad Sci U S A.  2001  98(14):8012-7  PubMed ID: 11416159   DOI: 10.1073/pnas.121616198
3078  Takahashi S, Takahashi Y, Ito K, Nagano T, Shibahara S, Miura T.  Positive and negative regulation of the human heme oxygenase-1 gene expression in cultured cells.  Biochim Biophys Acta  1999  1447(2-3):231-5  PubMed ID: 10542320   DOI: 10.1016/s0167-4781(99)00156-6
15093  Kitagawa M, Mukai H, Takahashi M, Ono Y.  The role of PKN in the regulation of alphaB-crystallin expression via heat shock transcription factor 1  Biochem Biophys Res Commun  1998  252(3):561-5  PubMed ID: 9837746   DOI: 10.1006/bbrc.1998.9694
3753  Murakami K, Nomiyama H, Miura R, Follens A, Fiten P, Van Coillie E, Van Damme J, Opdenakker G.  Structural and functional analysis of the promoter region of the human MCP-3 gene: transactivation of expression by novel recognition sequences adjacent to the transcription initiation site.  DNA Cell Biol  1997  16(2):173-83  PubMed ID: 9052738   DOI: 10.1089/dna.1997.16.173
1945  Kobayashi S, Satoh H, Takehana M.  Protective effects of glutathione isopropyl ester on the sensitivity of cultured cells to UVB irradiation.  Biol Pharm Bull  1995  18(9):1219-22  PubMed ID: 8845809   DOI: 10.1248/bpb.18.1219

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