Cell No. : Cell Name
RCB0191 : HeLa.S3
update : 2021/02/26
|
| Comment | Most famous cultured human cell line |
| Comment from the depositor | |
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
|
|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
Murakami, Yasufumi
|
| Year of deposit |
1987
|
| Animal |
_human
< Mammals
|
| Genus |
Homo
|
| Species |
sapiens
|
| Gender |
Female
|
| Tissue |
cervix
|
| Disease name |
cervical carcinoma
|
| Classification |
cancer
|
|
Lifespan |
infinite
|
| Morphology |
other
|
|
| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
|
Suspension cells
|
| Medium and additives |
|
MEM (suspension, 2mM L-Glutamine) + 10% CS
|
| Antibiotics |
|
Free
|
|
Passage method |
|
dilution
|
Culture information
|
Passage ratio |
|
1 : 8 split
|
| SC frequency |
|
Subculture : once/week, Medium Renewal : 2 times/week
|
| Temperature |
|
37
℃
|
| CO2 concentration |
|
5
%
|
| Freeze medium |
|
Medium + 10% DMSO
|
| Freezing method |
|
Slow freezing
|
| Mycoplasma |
|
(-)
|
| STR(human) |
|
OK
|
| Virus (HIV) |
|
(-)
|
| Isozyme |
|
LD, NP
|
| Chromosome mode |
|
56-271(50) : /64(2),65(2),66(6),67(20),68(8),69(2)/
|
| Reference information |
Reference |
5
|
| User's Publication |
15
|
| User's Publication |
|
12005
Kabayama H, Takeuchi M, Tokushige N, Muramatsu SI, Kabayama M, Fukuda M, Yamada Y, Mikoshiba K.
An ultra-stable cytoplasmic antibody engineered for in vivo applications.
Nat Commun
2020
PubMed ID: 31953402
|
|
4482
Roppongi M, Izumisawa M, Terasaki K, Muraki Y, Shozushima M.
18F-FDG and 11C-choline uptake in proliferating tumor cells is dependent on the cell cycle in vitro.
Ann Nucl Med
2019
33(4):237-243
PubMed ID: 30588580
|
|
6049
Sawada JI, Ishii H, Matsuno K, Sato M, Suzuki Y, Asai A.
Selective Inhibition of Spindle Microtubules by a Tubulin-Binding Quinazoline Derivative.
Mol Pharmacol
2019
96(5):609-618
PubMed ID: 31471455
|
|
10289
Murakami K, Zhao J, Yamasaki K, Miyagishi M.
Biochemical and structural features of extracellular vesicle-binding RNA aptamers.
Biomed Rep
2017
6:615-626
PubMed ID: 28584632
|
|
10026
Ojima D, Yasui A, Tohyama K, Tokuzumi K, Toriihara E, Ito K, Iwasaki A, Tomura T, Ojika M, Suenaga K.
Total Synthesis of Miuraenamides A and D.
J. Org. Chem.
2016
81:9886-9894
PubMed ID: 27662058
|
|
10366
Makoto Kimura
IRF2-binding protein-1 Is a JDP2 Ubiquitin Ligase and an Inhibitor of ATF2-dependent Transcription
FEBS Lett
2008
582(19):2833-7
PubMed ID: 18671972
|
|
8795
Engelhardt P, Merilainen J, Zhao F, Uchiyama S, Fukui K, Lehto VP.
Whole-mount immunoelectron tomography of chromosomes and cells.
Methods Mol. Biol.
2007
369:387-405
PubMed ID: 17656761
|
|
5595
Nakanishi A, Han X, Saito H, Taguchi K, Ohta Y, Imajoh-Ohmi S, Miki Y.
Interference with BRCA2, which localizes to the centrosome during S and early M phase, leads to abnormal nuclear division.
Biochem Biophys Res Commun
2007
355(1):34-40
PubMed ID: 17286961
|
|
3097
Uchiyama S, Kobayashi S, Takata H, Ishihara T, Hori N, Higashi T, Hayashihara K, Sone T, Higo D, Nirasawa T, Takao T, Matsunaga S, Fukui K.
Proteome analysis of human metaphase chromosomes.
J Biol Chem
2005
280(17):16994-7004
PubMed ID: 15687487
|
|
6278
Masanori SHOZUSHIMA, Reiko TSUTSUMI, Shigehiro SATO, Kazunori TERASAKI, Mitsuru IZUMISAWA, Hitoshi SATO, Satoru SHOJI, Kimio SAKAMAKI
Quantification of 11C-methionine Uptake During Proliferation of Cultured Human Cancer Cells
RADIOISOTOPES
2003
52:456-463
|
|
5010
Shimabukuro K, Ichinose S, Koike R, Kubota T, Yamaguchi M, Miyasaka M, Aso T.
Hepatocyte growth factor/scatter factor is implicated in the mode of stromal invasion of uterine squamous cervical cancer.
Gynecol Oncol
2001
83(2):205-15
PubMed ID: 11606073
|
|
2531
Masters JR, Thomson JA, Daly-Burns B, Reid YA, Dirks WG, Packer P, Toji LH, Ohno T, Tanabe H, Arlett CF, Kelland LR, Harrison M, Virmani A, Ward TH, Ayres KL, Debenham PG.
Short tandem repeat profiling provides an international reference standard for human cell lines.
Proc Natl Acad Sci U S A.
2001
98(14):8012-7
PubMed ID: 11416159
|
|
3078
Takahashi S, Takahashi Y, Ito K, Nagano T, Shibahara S, Miura T.
Positive and negative regulation of the human heme oxygenase-1 gene expression in cultured cells.
Biochim Biophys Acta
1999
1447(2-3):231-5
PubMed ID: 10542320
|
|
3753
Murakami K, Nomiyama H, Miura R, Follens A, Fiten P, Van Coillie E, Van Damme J, Opdenakker G.
Structural and functional analysis of the promoter region of the human MCP-3 gene: transactivation of expression by novel recognition sequences adjacent to the transcription initiation site.
DNA Cell Biol
1997
16(2):173-83
PubMed ID: 9052738
|
|
1945
Kobayashi S, Satoh H, Takehana M.
Protective effects of glutathione isopropyl ester on the sensitivity of cultured cells to UVB irradiation.
Biol Pharm Bull
1995
18(9):1219-22
PubMed ID: 8845809
|
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