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Cell No. : Cell Name
RCB0188 : OLME-104  update : 2022/03/07
CommentHB32C medaka treated with MNNG. From peritoneum. Amelanotic. Application consideration
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Etoh, Hisami
Originator Etoh, Hisami
Year of deposit 1987
Animal medaka < Fishes
Tissue melanoma
Classification transformed
Lifespan infinite
Morphology other
Cellosaurus(Expasy) CVCL_3767
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives M199 (20-25mM HEPES) + 15% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 4 split
SC frequency Subculture : once/2 weeks, Medium Renewal : 2 times/week
Temperature 25 ℃
CO2 concentration 0 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Reference information Reference 4
User's Publication 1

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1380  Matsudaira, H., Etoh, H., Hyodo-Taguchi, Y., Aoki, K.,Asami, K., Suyama, I., Muraiso, C., Yukawa, O. and Furuno-Fukushi  Medaka, A Useful Experimental System for Chemical and Enviromental Carcinogenesis  Recent Progress of Life Science Technology in Japan  1989  137-151   
1376  Muraiso, C., Nemoto, N., Asami, K., Etoh, H. and Matsudaira, H.  Enhanced Expression of Oncogenes in Cultured Medaka Tumor Cells    1988  211-214   
1377  Etoh, H., Suyama, I., Hyodo-Taguchi, Y. and Matsudaira, H.  Establishment and Charactaristics of Various Cell Lines from Medaka ( Teleostei )  Invertebrate and Fish Tissue Culture  1988  266-269   
1020  Etoh H, Hyodo-Taguchi Y, Aoki K, Murata M, Matsudaira H  Incidence of chromatoblastomas in aging goldfish (Carassius auratus).  J Natl Cancer Inst  1983  70:523-8  PubMed ID: 6572741  

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User's Publication
8193  Adachi K, Sumiyoshi K, Ariyasu R, Yamashita K, Zenke K, Okinaka Y.  Susceptibilities of medaka (Oryzias latipes) cell lines to a betanodavirus.  Virol. J.  2010  7:150  PubMed ID: 20624282   DOI: 10.1186/1743-422X-7-150

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