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Cell No. : Cell Name
RCB0187 : OLHE-131  update : 2022/03/07
CommentH04C medaka hepatoma pretreated with MAM-acetate. Application consideration
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Etoh, Hisami
Originator Etoh, Hisami
Year of deposit 1987
Animal _medaka < Fishes
Genus Oryzias
Species latipes
Tissue liver
Disease name hepatoma
Classification cancer
Year of origin 1983
Lifespan infinite
Morphology other
Cellosaurus(Expasy) CVCL_4873
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium M199 (20-25mM HEPES) + 15% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 2-4 split
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 25 ℃
CO2 concentration 0 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Isozyme LD, NP, MPI
deposit info
lot info
Reference information Reference 3
User's Publication 3

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1380  Matsudaira, H., Etoh, H., Hyodo-Taguchi, Y., Aoki, K.,Asami, K., Suyama, I., Muraiso, C., Yukawa, O. and Furuno-Fukushi  Medaka, A Useful Experimental System for Chemical and Enviromental Carcinogenesis  Recent Progress of Life Science Technology in Japan  1989  137-151   
1377  Etoh, H., Suyama, I., Hyodo-Taguchi, Y. and Matsudaira, H.  Establishment and Charactaristics of Various Cell Lines from Medaka ( Teleostei )  Invertebrate and Fish Tissue Culture  1988  266-269   
1020  Etoh H, Hyodo-Taguchi Y, Aoki K, Murata M, Matsudaira H  Incidence of chromatoblastomas in aging goldfish (Carassius auratus).  J Natl Cancer Inst  1983  70:523-8  PubMed ID: 6572741  

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User's Publication
16855  Zenke K, Okinaka Y.  Establishing an effective gene knockdown system using cultured cells of the model fish medaka ( Oryzias latipes)  Biol Methods Protoc  2022  7(1):bpac011  PubMed ID: 35685404   DOI: 10.1093/biomethods/bpac011
13908  Kawabe T, Kariya H, Hara S, Shirozu T, Shiraishi E, Mukai K, Yazawa T, Inoue S, Kitano T.  Transcriptional Regulation of Müllerian Inhibiting Substance (MIS) and Establishment of a Gonadal Somatic Cell Line Using mis-GFP Transgenic Medaka ( Oryzias latipes)  Front Endocrinol (Lausanne)  2020  11:578885  PubMed ID: 33133021   DOI: 10.3389/fendo.2020.578885
8193  Adachi K, Sumiyoshi K, Ariyasu R, Yamashita K, Zenke K, Okinaka Y.  Susceptibilities of medaka (Oryzias latipes) cell lines to a betanodavirus.  Virol. J.  2010  7:150  PubMed ID: 20624282   DOI: 10.1186/1743-422X-7-150

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