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Cell No. : Cell Name
RCB0186 : CAF  update : 2022/10/19
CommentPinna fibroblast
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Etoh, Hisami
Originator Etoh, Hisami
Year of deposit 1987
Animal gold fish < Fishes
Genus Carassius
Species auratus
Tissue pinna
Classification other
Year of origin 1977
Lifespan infinite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_R883
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives M199 (20-25mM HEPES) + 15% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 4 split
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 25 ℃
CO2 concentration 0 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Isozyme LD, NP
Images
deposit info
lot info
Reference information Reference 4
User's Publication 0


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Reference
1020  Etoh H, Hyodo-Taguchi Y, Aoki K, Murata M, Matsudaira H  Incidence of chromatoblastomas in aging goldfish (Carassius auratus).  J Natl Cancer Inst  1983  70:523-8  PubMed ID: 6572741
1023  Mitani H, Etoh H, Egami N  Resistance of a cultured fish cell line (CAF-MM1) to gamma irradiation.  Radiat Res  1982  89:334-47  PubMed ID: 7063616
1022  Mano Y, Mitani H, Etoh H, Egami N  Survival and photoreactivability of ultraviolet-irradiated cultured fish cells (CAF-MM1).  Radiat Res  1980  84:514-22  PubMed ID: 7454993
1021  Suyama, I. and Etoh, H.  A Cell Line Derived from the Fin of the Goldfish, Carassius Auratus.  Zoological Magazine  1979  83:321-324 

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