Cell No. : Cell Name
RCB0186 : CAF
update : 2022/10/19
|
| Comment | Pinna fibroblast |
| Comment from the depositor | |
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
|
|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
|
|
|
Basic information
|
Depositor |
Etoh, Hisami
|
| Originator |
Etoh, Hisami
|
| Year of deposit |
1987
|
| Animal |
_gold fish
< Fishes
|
| Genus |
Carassius
|
| Species |
auratus
|
| Tissue |
pinna
|
| Classification |
other
|
| Year of origin |
1977
|
|
Lifespan |
infinite
|
| Morphology |
fibroblast-like
|
| Cellosaurus(Expasy) |
CVCL_R883
|
|
| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
|
Adherent cells
|
| Culture medium |
|
M199 (20-25mM HEPES) + 15% FBS
|
| Antibiotics |
|
Free
|
|
Passage method |
|
0.25% Trypsin
|
Culture information
|
Passage ratio |
|
1 : 4 split
|
| SC frequency |
|
Subculture : once/week, Medium Renewal : 2 times/week
|
| Temperature |
|
25
℃
|
| CO2 concentration |
|
0
%
|
| Freeze medium |
|
Medium + 10% DMSO
|
| Freezing method |
|
Slow freezing
|
| Mycoplasma |
|
(-)
|
| Isozyme |
|
LD, NP
|
| Images |
deposit info | lot info |
|
|
|
| Reference information |
Reference |
4
|
| User's Publication |
0
|
| Reference |
|
1020
Etoh H, Hyodo-Taguchi Y, Aoki K, Murata M, Matsudaira H
Incidence of chromatoblastomas in aging goldfish (Carassius auratus).
J Natl Cancer Inst
1983
70:523-8
PubMed ID: 6572741
|
|
1023
Mitani H, Etoh H, Egami N
Resistance of a cultured fish cell line (CAF-MM1) to gamma irradiation.
Radiat Res
1982
89:334-47
PubMed ID: 7063616
|
|
1022
Mano Y, Mitani H, Etoh H, Egami N
Survival and photoreactivability of ultraviolet-irradiated cultured fish cells (CAF-MM1).
Radiat Res
1980
84:514-22
PubMed ID: 7454993
|
|
1021
Suyama, I. and Etoh, H.
A Cell Line Derived from the Fin of the Goldfish, Carassius Auratus.
Zoological Magazine
1979
83:321-324
|
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