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Cell No. : Cell Name
RCB0185 : CAEP  update : 2022/03/07
CommentGold fish erythrophoroma with 4n chromosomal mode
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Etoh, Hisami
Originator Etoh, Hisami
Year of deposit 1987
Animal gold fish < Fishes
Genus Carassius
Species auratus
Gender Unknown
Age at sampling 6 years
Tissue erythrophoroma
Disease name chromatoblastoma
Classification cancer
Year of origin 1984
Lifespan infinite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_4670
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives M199 (20-25mM HEPES) + 15% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 4 split
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 25 ℃
CO2 concentration 0 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Isozyme LD, NP
Reference information Reference 1
User's Publication 0


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Reference
1020  Etoh H, Hyodo-Taguchi Y, Aoki K, Murata M, Matsudaira H  Incidence of chromatoblastomas in aging goldfish (Carassius auratus).  J Natl Cancer Inst  1983  70:523-8  PubMed ID: 6572741

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