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Cell No. : Cell Name
RCB0184 : OLF-136  update : 2023/09/27
CommentFish (medaka) fin fibroblast. Application consideration
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Etoh, Hisami
Originator Etoh, Hisami
Year of deposit 1987
Animal _medaka < Fishes
Tissue fin
Classification other
Lifespan infinite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_3766
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium M199 (20-25mM HEPES) + 15% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 4 split
SC frequency Subculture : once/2 weeks, Medium Renewal : 2 times/week
Temperature 25 ℃
CO2 concentration 0 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Isozyme LD, NP
deposit info
lot info
Reference information Reference 3
User's Publication 4

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1380  Matsudaira, H., Etoh, H., Hyodo-Taguchi, Y., Aoki, K.,Asami, K., Suyama, I., Muraiso, C., Yukawa, O. and Furuno-Fukushi  Medaka, A Useful Experimental System for Chemical and Enviromental Carcinogenesis  Recent Progress of Life Science Technology in Japan  1989  137-151   
1377  Etoh, H., Suyama, I., Hyodo-Taguchi, Y. and Matsudaira, H.  Establishment and Charactaristics of Various Cell Lines from Medaka ( Teleostei )  Invertebrate and Fish Tissue Culture  1988  266-269   
1020  Etoh H, Hyodo-Taguchi Y, Aoki K, Murata M, Matsudaira H  Incidence of chromatoblastomas in aging goldfish (Carassius auratus).  J Natl Cancer Inst  1983  70:523-8  PubMed ID: 6572741  

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User's Publication
14281  Lescat L, Véron V, Mourot B, Péron S, Chenais N, Dias K, Riera-Heredia N, Beaumatin F, Pinel K, Priault M, Panserat S, Salin B, Guiguen Y, Bobe J, Herpin A, Seiliez I.  Chaperone-Mediated Autophagy in the Light of Evolution: Insight from Fish  Mol Biol Evol  2020  37(10):2887-2899  PubMed ID: 32437540   DOI: 10.1093/molbev/msaa127
8193  Adachi K, Sumiyoshi K, Ariyasu R, Yamashita K, Zenke K, Okinaka Y.  Susceptibilities of medaka (Oryzias latipes) cell lines to a betanodavirus.  Virol. J.  2010  7:150  PubMed ID: 20624282   DOI: 10.1186/1743-422X-7-150
8801  Su F, Osada Y, Ekker M, Chevrette M, Shimizu A, Asakawa S, Shiohama A, Sasaki T, Shimizu N, Yamanaka T, Sasado T, Mitani H, Geisler R, Kondoh H, Furutani-Seiki M.  Radiation hybrid maps of Medaka chromosomes LG 12, 17, and 22.  DNA Res.  2007  14:135-40  PubMed ID: 17591665   DOI: 10.1093/dnares/dsm012
1852  Saito, Hotaka and Shigeoka, Tadayoshi  Comparative Cytotoxicity of Chlorophenols to Cultured Fish Cells  Enviromental Toxicology and Chemistry  1994  13:1649-1650    DOI: 10.1002/etc.5620131014

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