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Cell No. : Cell Name
RCB0183 : RK13  update : 2021/01/08
CommentA cell line derived from kidney of rabbit. Susceptible to viruses such as rubella virus.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
Basic information Depositor Akaishi, Hiroomi
Year of deposit 1987
Animal rabbit < Mammals
Tissue kidney
Classification transformed
Memo_1 Mycoplasma was eliminated with MC-210.
Lifespan infinite
Morphology epithelial-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives MEM + 10% FBS + 0.1mM NEAA
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Isozyme LD, NP
Images
deposit info
lot info
Reference information Reference 1
User's Publication 5


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Reference
1076  Beale AJ, et al.  Rabbit Cells Susceptible to Rubella Virus.  Lancet  1963  282: 640-641 

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User's Publication
10142  Yuta Oshima, Kohei Seki, Masataka Shibuya, Yuki Naka, Tatsuya Yokoyama, Atsushi Sato  Soluble Human Intestinal Lactoferrin Receptor: Ca(2+)-Dependent Binding to Sepharose-Based Matrices  Biol Pharm Bull .   2016  39(3):435-9  PubMed ID: 26934934
3188  Oshiumi, Hiroyuki, Shida, Kyoko, Goitsuka, Ryo, Kimura, Yuko, Katoh, Jun, Ohba, Shinya, Tamaki, Yuichiroh, Hattori, Takashi, Yamada, Nozomi, Inoue, Norimitsu, Matsumoto, Misako, Mizuno, Shigeki, Seya, Tsukasa  Regulator of complement activation (RCA) locus in chicken: identification of chicken RCA gene cluster and functional RCA proteins.  J Immunol  2005  175:1724-34  PubMed ID: 16034113
2920  Kimura, Yuko, Inoue, Naokazu, Fukui, Aya, Oshiumi, Hiroyuki, Matsumoto, Misako, Nonaka, Mayumi, Kuratani, Shigeru, Fujita, Teizo, Nonaka, Masaru, Seya, Tsukasa  A short consensus repeat-containing complement regulatory protein of lamprey that participates in cleavage of lamprey complement 3.  J Immunol  2004  173:1118-28  PubMed ID: 15240701
10890  N Inoue, A Fukui, M Nomura, M Matsumoto, Y Nishizawa, K Toyoshima, T Seya  A Novel Chicken Membrane-Associated Complement Regulatory Protein: Molecular Cloning and Functional Characterization  J Immunol  2001  166(1):424-31  PubMed ID: 11123320
5428  Matsubasa T1, Takiguchi M, Matsuda I, Mori M.  Rat argininosuccinate lyase promoter: the dyad-symmetric CCAAT box sequence CCAATTGG in the promoter is recognized by NF-Y.  J Biochem  1994  116(5):1044-55  PubMed ID: 7896732



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