Cell No. : Cell Name
RCB0180 : MDBK
update : 2024/10/02
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| Comment | BSL2. A cell line derived from kidney of bovine. Information about BVDV (ATCC) . Information about BVDV (ECACC) . Information about BVDV (DSMZ) . |
| Comment from the depositor | |
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
Cell line to be handled at BSL 2(C-0070.pdf)
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
Akaishi, Hiroomi
|
| Year of deposit |
1987
|
| Animal |
_bovine
< Mammals
|
| Genus |
Bos
|
| Species |
taurus
|
| Tissue |
kidney
|
| Classification |
other
|
| Memo_1 |
Mycoplasma was eliminated with MC-110.
|
|
Lifespan |
infinite
|
| Morphology |
epithelial-like
|
| BSL |
BSL2
|
| Cellosaurus(Expasy) |
CVCL_0421
|
|
| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
|
Adherent cells
|
| Culture medium |
|
HamF12 + 10% FBS
|
| Antibiotics |
|
Free
|
|
Passage method |
|
0.25% Trypsin
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Culture information
|
Passage ratio |
|
1 : 8 split
|
| SC frequency |
|
Subculture : once/week, Medium Renewal : 2 times/week
|
| Temperature |
|
37
℃
|
| CO2 concentration |
|
5
%
|
| Freeze medium |
|
Medium + 10% DMSO
|
| Freezing method |
|
Slow freezing
|
| Mycoplasma/Acholeplasma |
|
(-)
|
| Animal PCR |
|
OK
|
| Isozyme |
|
LD, NP
|
| Chromosome mode |
|
47-53(50) : /48(1),49(2),50(2),51(8),52(35),53(1)
|
| Reference information |
Reference |
1
|
| User's Publication |
8
|
| User's Publication |
|
11948
Yamashiro T, Yasujima T, Ohta K, Inoue K, Yuasa H.
Identification of the amino acid residue responsible for the myricetin sensitivity of human proton-coupled folate transporter.
Sci Rep
2019
PubMed ID: 31792273
DOI: 10.1038/s41598-019-54367-9
|
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10762
Toji N, Koshi K, Furusawa T, Takahashi T, Ishiguro-Oonuma T, Kizaki K, Hashizume K.
A cell-based interferon-tau assay with an interferon-stimulated gene 15 promoter.
Biomed. Res.
2018
39:13-20
PubMed ID: 29467347
DOI: 10.2220/biomedres.39.13
|
|
11082
Suzuki T, Ikeda H, Mase M.
Restricted viral cDNA synthesis in cell lines that fail to support productive infection by bovine leukemia virus.
Arch. Virol.
2018
163(9):2415-2422
PubMed ID: 29796925
DOI: 10.1007/s00705-018-3887-6
|
|
8270
Sugawara Y, Matsumura T, Takegahara Y, Jin Y, Tsukasaki Y, Takeichi M, Fujinaga Y.
Botulinum hemagglutinin disrupts the intercellular epithelial barrier by directly binding E-cadherin.
J. Cell Biol.
2010
189:691-700
PubMed ID: 20457762
DOI: 10.1083/jcb.200910119
|
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15541
Susumu Muroya, Ikuyo Nakajima, Koichi Chikuni
Bovine Skeletal Muscle Cells Predominantly Express a Vascular Cell Adhesion Molecule-1 Seven-Ig Domain Splice Form
Zoological Science
2001
18:797-805
DOI: 10.2108/zsj.18.797
|
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2485
Sugimoto K, Fukuda R, Himeno M
Centromere/kinetochore localization of human centromere protein A (CENP-A) exogenously expressed as a fusion to green fluorescent protein.
Cell Struct Funct
2000
25:253-61
PubMed ID: 11129795
DOI: 10.1247/csf.25.253
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18051
Fujii M, Saijoh K, Kobayashi T, Fujii S, Lee MJ, Sumino K.
Analysis of bovine selenoprotein P-like protein gene and availability of metal responsive element (MRE) located in its promoter
Gene
1997
199(1-2):211-7
PubMed ID: 9358058
DOI: 10.1016/s0378-1119(97)00369-7
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17974
Nakamura TY, Yamamoto I, Nishitani H, Matozaki T, Suzuki T, Wakabayashi S, Shigekawa M, Goshima K.
Detachment of cultured cells from the substratum induced by the neutrophil-derived oxidant NH2Cl: synergistic role of phosphotyrosine and intracellular Ca2+ concentration
J Cell Biol
1995
131(2):509-24
PubMed ID: 7593175
DOI: 10.1083/jcb.131.2.509
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