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Cell No. : Cell Name
RCB0180 : MDBK  update : 2020/09/24
CommentKidney epithelial cell, equiv. to ATCC CRL6071
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, a prior written permission of the DEPOSITOR or the RIKEN BRC is necessary.
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC( regarding any kind of for-profit use.
Basic information Depositor Akaishi, Hiroomi
Year of deposit 1987
Animal bovine < Mammals
Genus Bos
Species taurus
Tissue kidney
Classification other
Memo_1 Mycoplasma was eliminated with MC-110.
Lifespan infinite
Morphology epithelial-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives HamF12 + 10% FBS
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Isozyme LD, NP
Chromosome mode 47-53(50) : /48(1),49(2),50(2),51(8),52(35),53(1)
Reference information Reference 1
User's Publication 5

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258  Madin, S.H. and Darby, N.B.  Established kidney cell lines of normal adult bovine and ovine origin  Proc Soc Exp Biol Med  1958  98:574  PubMed ID: 13567776

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User's Publication
11948  Yamashiro T, Yasujima T, Ohta K, Inoue K, Yuasa H.  Identification of the amino acid residue responsible for the myricetin sensitivity of human proton-coupled folate transporter.  Sci Rep  2019    PubMed ID: 31792273
10762  Toji N, Koshi K, Furusawa T, Takahashi T, Ishiguro-Oonuma T, Kizaki K, Hashizume K.  A cell-based interferon-tau assay with an interferon-stimulated gene 15 promoter.  Biomed. Res.  2018  39:13-20  PubMed ID: 29467347
11082  Suzuki T, Ikeda H, Mase M.  Restricted viral cDNA synthesis in cell lines that fail to support productive infection by bovine leukemia virus.  Arch. Virol.  2018  163(9):2415-2422  PubMed ID: 29796925
8270  Sugawara Y, Matsumura T, Takegahara Y, Jin Y, Tsukasaki Y, Takeichi M, Fujinaga Y.  Botulinum hemagglutinin disrupts the intercellular epithelial barrier by directly binding E-cadherin.  J. Cell Biol.  2010  189:691-700  PubMed ID: 20457762
2485  Sugimoto K, Fukuda R, Himeno M  Centromere/kinetochore localization of human centromere protein A (CENP-A) exogenously expressed as a fusion to green fluorescent protein.  Cell Struct Funct  2000  25:253-61  PubMed ID: 11129795

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