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細胞番号 : 細胞名
RCB0174 : SUSM-1  update : 2022/03/07
細胞特性(Comment:英)Transformed in vitro with carcinogen
細胞特性(日)ヒト正常2倍体線維芽細胞を発ガン剤によりin vitroで悪性転換した樹立細胞株。当室で脱マイコプラズマ汚染処理をしてある。
細胞特性(寄託者記述:英)
細胞特性(寄託者記述:日)
使用条件(英)A prior written permission of the approver(Originator) is necessary for any kind of use including academic use and for-profit use.
使用条件(日)営利目的か非営利目的かを問わず、いかなる使用につきましても、承諾者(樹立者)から承諾を得ることが必要です。
備考(英)
備考(日)
承諾者住所
×
Japanese
住所
700-8558 岡山県岡山市北区鹿田町2-5-1
岡山大学   医歯薬学総合研究科 細胞生物学分野
阪口 政清 先生
Fax. 086-235-7400

English
Address
Okayama University Graduate School of Medicine
Dentistry, and Pharmaceutical Sciences, Department of Cell Biology
2-5-1 shikata-cho , Kita-ku , Okayama-shi, Okayama 700-8558 Japan
Dr.SAKAGUCHI Masakiyo
Fax. +81-86-235-7400
提供申込書類(英) Order Form(C-0005.pdf)   Approval Form(C-0006.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
提供申込書類(日) 依頼書C-0001.pdf   承諾書C-0002.pdf   同意書(非営利学術目的)C-0003.pdf   同意書(営利目的)C-0003p.pdf  
提供同意書は、使用機関の種類や目的に応じて、非営利学術目的 (C-XXXX) と営利目的 (C-XXXXp) の2種類があります。該当する提供同意書をご使用ください(詳細)。特許等の取得及び商業利用等は事前に必ず cellbank.brc@riken.jp までご連絡ください。
提供手数料 手数料とお支払いについてはこちらをご覧ください。
細胞基本情報 寄託者 Ohno, Tadao
樹立者 Huh, Nam, Ho (Nanba, Masayoshi)
寄託日 1987
動物種 human < Mammals
属名 Homo
種名 sapiens
人種 Japanese
採取組織 lung
細胞分類 transformed
入手歴 Namba(Kawasaki U.)-Hoshi, H.(TMycoplasma was decontaminated was decontaminated in RCB.
メモ_1 Mycoplasma was eliminated with MC-110.
細胞寿命 infinite
細胞形態 fibroblast-like
Cellosaurus(Expasy) CVCL_4903
細胞培養・検査情報
寄託時情報
ロット情報
培地・試薬情報 培地・試薬一覧はこちらをご覧ください。
培養形態 Adherent cells
培地 HF-RITC80-7 (or MEM ALPHA) + 10% FBS
抗生物質 Free
継代方法 0.25% Trypsin
継代密度 1 : 8 split
継代・培地交換頻度 Subculture : once/week, Medium Renewal : 2 times/week
培養最適温度 37 ℃
二酸化炭素濃度 5 %
凍結培地 Medium + 10% DMSO
凍結方法 Slow freezing
マイコプラズマ (-)
アイソザイム検査 LD, NP
染色体検査 83-244(50) : /123(6),124(2),125(3),126(2),127(3)/
個体識別検査 OK
文献情報 Reference(英) 18件
Reference(日) 1件
利用者成果(英) 1件
利用者成果(日) 0件

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Reference(英)
4633  Gao C, Miyazaki M, Kondo T, Tsuji T, Sakaguchi M, Namba M.  Overexpression of platelet-derived growth factor B and downregulation of PDGF-receptor alpha in human immortalized fibroblasts.  Int J Oncol  2001  18(4):871-5  PubMed ID: 11251187   DOI: 10.3892/ijo.18.4.871
4621  Sugihara S, Mihara K, Marunouchi T, Inoue H, Namba M.  Telomere elongation observed in immortalized human fibroblasts by treatment with 60Co gamma rays or 4-nitroquinoline 1-oxide.  Hum Genet  1996  97(1):1-6  PubMed ID: 8557247   DOI: 10.1007/bf00218823
4632  Iijima M, Kano Y, Nohno T, Namba M.  Cloning of cDNA with possible transcription factor activity at the G1-S phase transition in human fibroblast cell lines.  Acta Med Okayama  1996  50(2):73-7  PubMed ID: 8744932   DOI: 10.18926/AMO/30489
4629  Ogata T, Oshimura M, Namba M, Fujii M, Oishi M, Ayusawa D.  Genetic complementation of the immortal phenotype in group D cell lines by introduction of chromosome 7.  Jpn J Cancer Res  1995  86(1):35-40  PubMed ID: 7737907   DOI: 10.1111/j.1349-7006.1995.tb02985.x
4630  Kawashima K, Mihara K, Usuki H, Shimizu N, Namba M.  Transfected mutant p53 gene increases X-ray-induced cell killing and mutation in human fibroblasts immortalized with 4-nitroquinoline 1-oxide but does not induce neoplastic transformation of the cells.  Int J Cancer  1995  61(1):76-9  PubMed ID: 7705936   DOI: 10.1002/ijc.2910610113
4631  Kondo T, Mihara K, Inoue Y, Iijima M, Namba M.  Two-dimensional electrophoretic analysis of down-regulated proteins in human fibroblasts immortalized by treatment with either 4-nitroquinoline 1-oxide or 60Co gamma rays.  Electrophoresis  1995  16(6):1067-73  PubMed ID: 7498129   DOI: 10.1002/elps.11501601177
4624  Ogata T, Ayusawa D, Namba M, Takahashi E, Oshimura M, Oishi M.  Chromosome 7 suppresses indefinite division of nontumorigenic immortalized human fibroblast cell lines KMST-6 and SUSM-1.  Mol Cell Biol  1993  13(10):6036-43  PubMed ID: 8105370   DOI: 10.1128/mcb.13.10.6036
4628  Namba M, Iijima M, Kondo T, Jahan I, Mihara K.  Immortalization of normal human cells is a multistep process and a rate limiting step of neoplastic transformation of the cells.  Hum Cell  1993  6(4):253-9  PubMed ID: 8148305  
4627  Bai L, Naomoto Y, Miyazaki M, Orita K, Namba M.  Antiproliferative effects of suramin on human cancer cells in vitro and in vivo.  Acta Med Okayama  1992  46(6):457-63  PubMed ID: 1485540   DOI: 10.18926/AMO/32642
4626  Bai LY, Miyazaki M, Namba M.  Effects of suramin on cell proliferation of various types of human malignant cells  Gan To Kagaku Ryoho  1991  18(3):387-91  PubMed ID: 1848419  
4619  Cornelis JJ, Becquart P, Duponchel N, Salomé N, Avalosse BL, Namba M, Rommelaere J.  Transformation of human fibroblasts by ionizing radiation, a chemical carcinogen, or simian virus 40 correlates with an increase in susceptibility to the autonomous parvoviruses H-1 virus and minute virus of mice.  J Virol  1988  62(5):1679-86  PubMed ID: 2833618   DOI: 10.1128/JVI.62.5.1679-1686.1988
4620  McCormick JJ, Maher VM.  Towards an understanding of the malignant transformation of diploid human fibroblasts.  Mutat Res  1988  199(2):273-91  PubMed ID: 3287148   DOI: 10.1016/0027-5107(88)90209-6
4622  Namba M, Nishitani K, Fukushima F, Kimoto T.  Multistep carcinogenesis of normal human fibroblasts. Human fibroblasts immortalized by repeated treatment with Co-60 gamma rays were transformed into tumorigenic cells with Ha-ras oncogenes.  Anticancer Res  1988  8(5A):947-58  PubMed ID: 3140712  
4625  Namba M, Karai M, Kimoto T.  Comparison of major cytoskeletons among normal human fibroblasts, immortal human fibroblasts transformed by exposure to Co-60 gamma rays, and the latter cells made tumorigenic by treatment with Harvey murine sarcoma virus.  Exp Gerontol  1987  22(3):179-86  PubMed ID: 3305051   DOI: 10.1016/0531-5565(87)90038-6
1559  Ohno T, Kako R, Sato C, Ohkawa A  Distinction of G0 cells from senescent cells in cultures of non-cycling human fetal lung fibroblasts by anti-MAP-1 monoclonal antibody staining.  Exp Cell Res  1986  163:309-16  PubMed ID: 3956580   DOI: 10.1016/0014-4827(86)90062-5
3176  Namba M, Kusaka T, Fukushima F, Kimoto T  Differential growth response of normal human diploid fibroblasts and in vitro transformed human fibroblasts in serum-free defined culture medium.  Int J Cancer  1984  34:645-9  PubMed ID: 6500743   DOI: 10.1002/ijc.2910340510
1822  Hoshi H, Kan M, Yamane I, Ohno T, Namba M  Differential growth requirements of serum factors (bovine serum albumin and low density lipoprotein) for normal and transformed human cells in a serum-free culture.  Cell Struct Funct  1983  8:67-75  PubMed ID: 6667512   DOI: 10.1247/csf.8.67
4618  Stein GH, Yanishevsky RM.  Quiescent human diploid cells can inhibit entry into S phase in replicative nuclei in heterodikaryons.  Proc Natl Acad Sci U S A  1981  78(5):3025-9  PubMed ID: 6265932   DOI: 10.1073/pnas.78.5.3025

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Reference(日)
4623  中林一彦, 鮎沢大  細胞老化遺伝子のマッピング  蛋白質核酸酵素  1996  41:237-244   

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利用者成果(英)
13730  Yatabe N, Kyo S, Kondo S, Kanaya T, Wang Z, Maida Y, Takakura M, Nakamura M, Tanaka M, Inoue M.  2-5A antisense therapy directed against human telomerase RNA inhibits telomerase activity and induces apoptosis without telomere impairment in cervical cancer cells  Cancer Gene Ther  2002  9(7):624-30  PubMed ID: 12082463   DOI: 10.1038/sj.cgt.7700479

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利用者成果(日)



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