Search
CELL BANK Website

Back Back
Cell No. : Cell Name
RCB0174 : SUSM-1  update : 2022/03/07
CommentTransformed in vitro with carcinogen
Comment from the depositor
Terms and conditionsA prior written permission of the approver(Originator) is necessary for any kind of use including academic use and for-profit use.
Remarks
approver's address
×
English
Address
Okayama University Graduate School of Medicine
Dentistry, and Pharmaceutical Sciences, Department of Cell Biology
2-5-1 shikata-cho , Kita-ku , Okayama-shi, Okayama 700-8558 Japan
Dr.SAKAGUCHI Masakiyo
Fax. +81-86-235-7400
Order Form Order Form(C-0005.pdf)   Approval Form(C-0006.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Ohno, Tadao
Originator Huh, Nam, Ho (Nanba, Masayoshi)
Year of deposit 1987
Animal human < Mammals
Genus Homo
Species sapiens
Race Japanese
Tissue lung
Classification transformed
History Namba(Kawasaki U.)-Hoshi, H.(TMycoplasma was decontaminated was decontaminated in RCB.
Memo_1 Mycoplasma was eliminated with MC-110.
Lifespan infinite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_4903
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives HF-RITC80-7 (or MEM ALPHA) + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Isozyme LD, NP
Chromosome mode 83-244(50) : /123(6),124(2),125(3),126(2),127(3)/
STR(human) OK
Reference information Reference 18
User's Publication 1


To topTop
Reference
4633  Gao C, Miyazaki M, Kondo T, Tsuji T, Sakaguchi M, Namba M.  Overexpression of platelet-derived growth factor B and downregulation of PDGF-receptor alpha in human immortalized fibroblasts.  Int J Oncol  2001  18(4):871-5  PubMed ID: 11251187
4621  Sugihara S, Mihara K, Marunouchi T, Inoue H, Namba M.  Telomere elongation observed in immortalized human fibroblasts by treatment with 60Co gamma rays or 4-nitroquinoline 1-oxide.  Hum Genet  1996  97(1):1-6  PubMed ID: 8557247
4632  Iijima M, Kano Y, Nohno T, Namba M.  Cloning of cDNA with possible transcription factor activity at the G1-S phase transition in human fibroblast cell lines.  Acta Med Okayama  1996  50(2):73-7  PubMed ID: 8744932
4629  Ogata T, Oshimura M, Namba M, Fujii M, Oishi M, Ayusawa D.  Genetic complementation of the immortal phenotype in group D cell lines by introduction of chromosome 7.  Jpn J Cancer Res  1995  86(1):35-40  PubMed ID: 7737907
4630  Kawashima K, Mihara K, Usuki H, Shimizu N, Namba M.  Transfected mutant p53 gene increases X-ray-induced cell killing and mutation in human fibroblasts immortalized with 4-nitroquinoline 1-oxide but does not induce neoplastic transformation of the cells.  Int J Cancer  1995  61(1):76-9  PubMed ID: 7705936
4631  Kondo T, Mihara K, Inoue Y, Iijima M, Namba M.  Two-dimensional electrophoretic analysis of down-regulated proteins in human fibroblasts immortalized by treatment with either 4-nitroquinoline 1-oxide or 60Co gamma rays.  Electrophoresis  1995  16(6):1067-73  PubMed ID: 7498129
4624  Ogata T, Ayusawa D, Namba M, Takahashi E, Oshimura M, Oishi M.  Chromosome 7 suppresses indefinite division of nontumorigenic immortalized human fibroblast cell lines KMST-6 and SUSM-1.  Mol Cell Biol  1993  13(10):6036-43  PubMed ID: 8105370
4628  Namba M, Iijima M, Kondo T, Jahan I, Mihara K.  Immortalization of normal human cells is a multistep process and a rate limiting step of neoplastic transformation of the cells.  Hum Cell  1993  6(4):253-9  PubMed ID: 8148305
4627  Bai L, Naomoto Y, Miyazaki M, Orita K, Namba M.  Antiproliferative effects of suramin on human cancer cells in vitro and in vivo.  Acta Med Okayama  1992  46(6):457-63  PubMed ID: 1485540
4626  Bai LY, Miyazaki M, Namba M.  Effects of suramin on cell proliferation of various types of human malignant cells  Gan To Kagaku Ryoho  1991  18(3):387-91  PubMed ID: 1848419
4619  Cornelis JJ, Becquart P, Duponchel N, Salomé N, Avalosse BL, Namba M, Rommelaere J.  Transformation of human fibroblasts by ionizing radiation, a chemical carcinogen, or simian virus 40 correlates with an increase in susceptibility to the autonomous parvoviruses H-1 virus and minute virus of mice.  J Virol  1988  62(5):1679-86  PubMed ID: 2833618
4620  McCormick JJ, Maher VM.  Towards an understanding of the malignant transformation of diploid human fibroblasts.  Mutat Res  1988  199(2):273-91  PubMed ID: 3287148
4622  Namba M, Nishitani K, Fukushima F, Kimoto T.  Multistep carcinogenesis of normal human fibroblasts. Human fibroblasts immortalized by repeated treatment with Co-60 gamma rays were transformed into tumorigenic cells with Ha-ras oncogenes.  Anticancer Res  1988  8(5A):947-58  PubMed ID: 3140712
4625  Namba M, Karai M, Kimoto T.  Comparison of major cytoskeletons among normal human fibroblasts, immortal human fibroblasts transformed by exposure to Co-60 gamma rays, and the latter cells made tumorigenic by treatment with Harvey murine sarcoma virus.  Exp Gerontol  1987  22(3):179-86  PubMed ID: 3305051
1559  Ohno T, Kako R, Sato C, Ohkawa A  Distinction of G0 cells from senescent cells in cultures of non-cycling human fetal lung fibroblasts by anti-MAP-1 monoclonal antibody staining.  Exp Cell Res  1986  163:309-16  PubMed ID: 3956580
3176  Namba M, Kusaka T, Fukushima F, Kimoto T  Differential growth response of normal human diploid fibroblasts and in vitro transformed human fibroblasts in serum-free defined culture medium.  Int J Cancer  1984  34:645-9  PubMed ID: 6500743
1822  Hoshi H, Kan M, Yamane I, Ohno T, Namba M  Differential growth requirements of serum factors (bovine serum albumin and low density lipoprotein) for normal and transformed human cells in a serum-free culture.  Cell Struct Funct  1983  8:67-75  PubMed ID: 6667512
4618  Stein GH, Yanishevsky RM.  Quiescent human diploid cells can inhibit entry into S phase in replicative nuclei in heterodikaryons.  Proc Natl Acad Sci U S A  1981  78(5):3025-9  PubMed ID: 6265932

To topTop
User's Publication
13730  Yatabe N, Kyo S, Kondo S, Kanaya T, Wang Z, Maida Y, Takakura M, Nakamura M, Tanaka M, Inoue M.  2-5A antisense therapy directed against human telomerase RNA inhibits telomerase activity and induces apoptosis without telomere impairment in cervical cancer cells  Cancer Gene Ther  2002  9(7):624-30  PubMed ID: 12082463



Back Back Return Top Page