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Cell No. : Cell Name
RCB0171 : 7T1  update : 2022/03/07
CommentContact inhibited, may transform spontaneously
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Ohno, Tadao
Originator Matsumura, Toshiharu
Year of deposit 1987
Animal mouse < Mammals
Strain name BALB/c
Age at sampling embryo
Tissue embryo/fetus, whole
Classification other
Lifespan infinite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_4E63
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM (low glucose) + 10% FBS
Antibiotics Free
Passage method dispase (2000U/ml)
Culture information Passage cell No 1 x 10^(5) cells/60 mm dish
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
Chromosome mode 77-174(50) : /81(3),82(6),83(11),84(3),85(9),86(2)/
Reference information Reference 1
User's Publication 0


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Reference
1066  Matsumura, T., Miyashita, S. and Ohno, T.  Conversion of proliferation and production of the colony stimulating factor during serial passage of mouse fibroblasts in culture.  Cell Struct Funct  1979  4:267-274    DOI: 10.1247/csf.4.267

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