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Cell No. : Cell Name
RCB0165 : REF-RGB  update : 2021/05/20
CommentSuitable as feeders for hybridoma cloning
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Murakami, Yasufumi
Originator Murakami, Yasufumi
Year of deposit 1987
Animal _rat < Mammals
Strain name Wistar
Genus Rattus
Species norvegicus
Age at sampling embryo
Tissue embryo
Classification semi-normal
Lifespan infinite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_U898
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM (low glucose) + 10% FBS
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Isozyme LD, NP, G6PD
Chromosome mode 35-42(50) : 35(1),39(1),40(1),41(4),42(43)
Reference information Reference 0
User's Publication 1


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Reference

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User's Publication
15595  Murata-Hori M, Fukuta Y, Ueda K, Iwasaki T, Hosoya H.  HeLa ZIP kinase induces diphosphorylation of myosin II regulatory light chain and reorganization of actin filaments in nonmuscle cells  Oncogene  2001  20(57):8175-8  PubMed ID: 11781833



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