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Cell No. : Cell Name
RCB0164 : RGB3T3-5  update : 2021/01/08
CommentSiblings of RGB3T3-1, contact-inhibited
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Ohno, Tadao
Originator Ohno, Tadao
Year of deposit 1987
Original cell RGB3T3
Animal _mouse < Mammals
Strain name BALB/c
Age at sampling embryo
Tissue embryo
Classification other
Lifespan infinite
Morphology fibroblast-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM (low glucose) + 10% FBS
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage cell No 3 x 10^(5) cells/60 mm dish
SC frequency Subculture : once/3-4 days
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
Chromosome mode 56-125(50) : /62(3),63(4),64(1),66(3),67(3),68(1)/
Reference information Reference 0
User's Publication 1


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Reference

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User's Publication
3748  Yuasa K, Hijikata T.  Distal regulatory element of the STAT1 gene potentially mediates positive feedback control of STAT1 expression.  Genes Cells  2016  21(1):25-40  PubMed ID: 26592235



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