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Cell No. : Cell Name
RCB0163 : RGB3T3-1  update : 2022/03/07
CommentOriginal cloned line from RCB, just like BALB/3T3
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Ohno, Tadao
Originator Ohno, Tadao
Year of deposit 1987
Original cell RGB3T3
Animal mouse < Mammals
Strain name BALB/c
Age at sampling embryo
Tissue embryo
Classification other
Lifespan infinite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_T375
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM (low glucose) + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage cell No 3 x 10^(5) cells/60 mm dish
SC frequency Subculture : once/3-4 days
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
Chromosome mode 58-134(50) : /64(8),65(3),66(4),67(8),68(6),70(6)/
deposit info
lot info
Reference information Reference 0
User's Publication 1

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User's Publication
9101  Keisuke Sasaki, Akihiro Yoneda, Akinori Ninomiya, Manabu Kawahara, Tomomasa Watanabe  Both Antiviral Activity and Intracellular Localization of Chicken Mx Protein Depend on a Polymorphism at Amino Acid Position 631  Biochem Biophys Res Commun  2013  430 (1), 161-6  PubMed ID: 23201406   DOI: 10.1016/j.bbrc.2012.11.053

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