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Cell No. : Cell Name
RCB0162 : NHSF46  update : 2022/03/07
CommentDiploid fibroblast from normal Japanese skin.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Ohno, Tadao
Originator Fujiwara, Yoshisada
Year of deposit 1986
Animal human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Unknown
Tissue skin
Classification normal
Lifespan finite
Crisis PDL 45 PDL
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_G283
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives MEM ALPHA + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 4 split
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Virus (HIV) (-)
Isozyme LD, NP
Chromosome mode 41-91(50) : 41(1),43(1),44(1),45(3),46(41),47(2)/
STR(human) OK
Images
deposit info
lot info
Reference information Reference 0
User's Publication 7


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Reference

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User's Publication
5631  Uto T, Sakamoto A, Tung NH, Fujiki T, Kishihara K, Oiso S, Kariyazono H, Morinaga O, Shoyama Y.  Anti-Proliferative Activities and Apoptosis Induction by Triterpenes Derived from Eriobotrya japonica in Human Leukemia Cell Lines.  Int J Mol Sci  2013  14(2):4106-20  PubMed ID: 23429195   DOI: 10.3390/ijms14024106
7933  Yoshimitsu M, Higuchi K, Miyata M, Devine S, Mattman A, Sirrs S, Medin JA, Tei C, Takenaka T.  Identification of novel mutations in the α-galactosidase A gene in patients with Fabry disease: pitfalls of mutation analyses in patients with low α-galactosidase A activity.  J Cardiol  2011  57:345-53  PubMed ID: 21333496   DOI: 10.1016/j.jjcc.2010.12.004
7956  Uchino T, Ikarashi Y, Nishimura T.  Effects of coating materials and size of titanium dioxide particles on their cytotoxicity and penetration into the cellular membrane.  J Toxicol Sci  2011  36:95-100  PubMed ID: 21297346   DOI: 10.2131/jts.36.95
8330  Maeda H, Nakano T, Tomokiyo A, Fujii S, Wada N, Monnouchi S, Hori K, Akamine A.  Mineral trioxide aggregate induces bone morphogenetic protein-2 expression and calcification in human periodontal ligament cells.  J Endod  2010  36:647-52  PubMed ID: 20307738   DOI: 10.1016/j.joen.2009.12.024
8672  Uchino T, Takezawa T, Ikarashi Y.  Reconstruction of three-dimensional human skin model composed of dendritic cells, keratinocytes and fibroblasts utilizing a handy scaffold of collagen vitrigel membrane.  Toxicol In Vitro  2009  23:333-7  PubMed ID: 19121381   DOI: 10.1016/j.tiv.2008.12.003
3177  Tsukamoto, Hiroshi, Horiuchi, Takahiko, Kokuba, Hisashi, Nagae, Shonosuke, Nishizaka, Hiroaki, Sawabe, Takuya, Harashima, Shin-ichi, Himeji, Daisuke, Koyama, Takako, Otsuka, Junji, Mitoma, Hiroki, Kimoto, Yasutaka, Hashimura, Chinami, Kitano, Etsuko, Kitamura, Hajime, Furue, Masutaka, Harada, Mine  Molecular analysis of a novel hereditary C3 deficiency with systemic lupus erythematosus.  Biochem Biophys Res Commun  2005  330:298-304  PubMed ID: 15781264   DOI: 10.1016/j.bbrc.2005.02.159
6269  Kawashima H, Li YF, Watanabe N, Hirose J, Hirose M, Miyasaka M.  Identification and characterization of ligands for L-selectin in the kidney. I. Versican, a large chondroitin sulfate proteoglycan, is a ligand for L-selectin.  Int Immunol  1999  11(3):393-405  PubMed ID: 10221651   DOI: 10.1093/intimm/11.3.393



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