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Cell No. : Cell Name
RCB0159 : HS-K  update : 2024/05/30
CommentNormal diploid fibroblast from Japanese
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Ohno, Tadao
Originator Kan, Mikio
Year of deposit 1987
Animal _human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Female
Tissue skin
Classification normal
History Kan(Tohoku-U)-Ohno(RCB)
Lifespan finite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_K100
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium MEM ALPHA + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 4 split
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Virus (HIV) (-)
Isozyme LD, NP, G6PD
Chromosome mode 42-47(50) : 42(1),43(5),44(2),45(13),46(28),47(1)
STR(human) OK
deposit info
lot info
Reference information Reference 0
User's Publication 5

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User's Publication
12586  Sato K, Shi L, Ito F, Ohara Y, Motooka Y, Tanaka H, Mizuno M, Hori M, Hirayama T, Hibi H, Toyokuni S.  Non-thermal plasma specifically kills oral squamous cell carcinoma cells in a catalytic Fe(II)-dependent manner  J Clin Biochem Nutr  2019  65(1):8-15  PubMed ID: 31379408   DOI: 10.3164/jcbn.18-91
5021  Takano H1, Nakamura T1, Tsuchikawa T1, Kushibiki T, Hontani K, Inoko K, Takahashi M, Sato S, Abe H, Takeuchi S, Sato N, Hiraoka K, Nishihara H, Shichinohe T, Hirano S.  Inhibition of Eph receptor A4 by 2,5-dimethylpyrrolyl benzoic acid suppresses human pancreatic cancer growing orthotopically in nude mice.  Oncotarget  2015  6(38):41063-76  PubMed ID: 26516928   DOI: 10.18632/oncotarget.5729
16960  Xin-Hui Xing, Akinori Ono, Kazuhiko Miyanaga, Yasunori Tanji, Hajime Unno  A kinetic model for growth of callus derived from Eucommia ulmoides aiming at mass production of a factor enhancing collagen synthesis of animal cells  Mathematics and Computers in Simulation  2001  56:463-474    DOI: 10.1016/s0378-4754(01)00315-9
1841  Kobayashi S, Hirota Y, Sayato-Suzuki J, Takehana M, Nishimura H, Nishimura N, Tohyama C  Possible role of metallothionein in the cellular defense mechanism against UVB irradiation in neonatal human skin fibroblasts.  Photochem Photobiol  1994  59:650-6  PubMed ID: 8066125   DOI: 10.1111/j.1751-1097.1994.tb09671.x
5429  Yoshiura M1, Murakami H, Tashiro H, Kurisu K.  Production of a human monoclonal antibody to normal basal and squamous cell carcinoma-associated antigen.  J Oral Pathol Med  1993  22(10):451-8  PubMed ID: 7510337   DOI: 10.1111/j.1600-0714.1993.tb00124.x

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