Cell No. : Cell Name
RCB0159 : HS-K
update : 2024/10/04
|
| Comment | Normal diploid fibroblast from Japanese |
| Comment from the depositor | |
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
Ohno, Tadao
|
| Originator |
Kan, Mikio
|
| Year of deposit |
1987
|
| Animal |
_human
< Mammals
|
| Genus |
Homo
|
| Species |
sapiens
|
| Race |
Japanese
|
| Gender |
Female
|
| Tissue |
skin
|
| Classification |
normal
|
| History |
Kan(Tohoku-U)-Ohno(RCB)
|
|
Lifespan |
finite
|
| Morphology |
fibroblast-like
|
| Cellosaurus(Expasy) |
CVCL_K100
|
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| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
|
Adherent cells
|
| Culture medium |
|
MEM ALPHA + 10% FBS
|
| Antibiotics |
|
Free
|
|
Passage method |
|
0.25% Trypsin
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Culture information
|
Passage ratio |
|
1 : 4 split
|
| SC frequency |
|
Subculture : once/week, Medium Renewal : 2 times/week
|
| Temperature |
|
37
℃
|
| CO2 concentration |
|
5
%
|
| Freeze medium |
|
Medium + 10% DMSO
|
| Freezing method |
|
Slow freezing
|
| Mycoplasma/Acholeplasma |
|
(-)
|
| Virus (HIV) |
|
(-)
|
| Isozyme |
|
LD, NP, G6PD
|
| Chromosome mode |
|
42-47(50) : 42(1),43(5),44(2),45(13),46(28),47(1)
|
| STR(human) |
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OK
|
| Images |
deposit info | lot info |
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| Reference information |
Reference |
0
|
| User's Publication |
6
|
| User's Publication |
|
12586
Sato K, Shi L, Ito F, Ohara Y, Motooka Y, Tanaka H, Mizuno M, Hori M, Hirayama T, Hibi H, Toyokuni S.
Non-thermal plasma specifically kills oral squamous cell carcinoma cells in a catalytic Fe(II)-dependent manner
J Clin Biochem Nutr
2019
65(1):8-15
PubMed ID: 31379408
DOI: 10.3164/jcbn.18-91
|
|
5021
Takano H1, Nakamura T1, Tsuchikawa T1, Kushibiki T, Hontani K, Inoko K, Takahashi M, Sato S, Abe H, Takeuchi S, Sato N, Hiraoka K, Nishihara H, Shichinohe T, Hirano S.
Inhibition of Eph receptor A4 by 2,5-dimethylpyrrolyl benzoic acid suppresses human pancreatic cancer growing orthotopically in nude mice.
Oncotarget
2015
6(38):41063-76
PubMed ID: 26516928
DOI: 10.18632/oncotarget.5729
|
|
16960
Xin-Hui Xing, Akinori Ono, Kazuhiko Miyanaga, Yasunori Tanji, Hajime Unno
A kinetic model for growth of callus derived from Eucommia ulmoides aiming at mass production of a factor enhancing collagen synthesis of animal cells
Mathematics and Computers in Simulation
2001
56:463-474
DOI: 10.1016/s0378-4754(01)00315-9
|
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2312
Akinori Ono, Xin-Hui Xing, Hajime Unno
Cultured-Animal-Cell Assay for Collagen-Synthesis-Activation Factor Produced by Callus of Woody Plant Eucommia Ulmoides
Animal Cell Technology: Basic & Applied Aspects
1998
385-389
DOI: 10.1007/978-94-011-5161-0_66
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1841
Kobayashi S, Hirota Y, Sayato-Suzuki J, Takehana M, Nishimura H, Nishimura N, Tohyama C
Possible role of metallothionein in the cellular defense mechanism against UVB irradiation in neonatal human skin fibroblasts.
Photochem Photobiol
1994
59:650-6
PubMed ID: 8066125
DOI: 10.1111/j.1751-1097.1994.tb09671.x
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5429
Yoshiura M1, Murakami H, Tashiro H, Kurisu K.
Production of a human monoclonal antibody to normal basal and squamous cell carcinoma-associated antigen.
J Oral Pathol Med
1993
22(10):451-8
PubMed ID: 7510337
DOI: 10.1111/j.1600-0714.1993.tb00124.x
|
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