Cell No. : Cell Name RCB0156 : 305M  update : 2022/12/14
Comment			Normal human skin fibroblast
Comment from the depositor
Terms and conditions			Aging reseach group use only.
Remarks
Order Form			Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)
			Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
	Basic information	Depositor	Ohno, Tadao
		Originator	Matsumura, Toshiharu
		Year of deposit	1987
		Animal	_human < Mammals
		Genus	Homo
		Species	sapiens
		Race	Japanese
		Gender	Female
		Age at sampling	26 years
		Tissue	skin
		Classification	normal
		Lifespan	finite
		Crisis PDL	47 PDL
		Morphology	fibroblast-like
		Cellosaurus(Expasy)	CVCL_6531
			deposit info	lot info
		Medium	Medium List
		Culture type		Adherent cells
		Culture medium		HF-RITC80-7 (or MEM ALPHA) + 10% FBS
		Antibiotics		Free
		Passage method		0.25% Trypsin
	Culture information	Passage ratio		1 : 8 split
		SC frequency		Subculture : 2 times/week
		Temperature		37 ℃
		CO2 concentration		5 %
		Freeze medium		Medium + 10% DMSO
		Freezing method		Slow freezing
		Mycoplasma/Acholeplasma		(-)
		Virus (HIV)		(-)
		Isozyme		LD, NP, G6PD
		Chromosome mode		43-47(50) : 43(1),44(4),45(7),46(36),47(2)
		STR(human)		OK
Images			deposit info	lot info
Reference information		Reference	1
		User's Publication	2

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Reference

1495  Ohno T, Yamada M  Growth stimulation by direct cell-cell contact (contact stimulation) in normal human lung fibroblasts.  Biomed Pharmacother  1984  38:337-43  PubMed ID: 6525431

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User's Publication

17433  Sugiura R, Hamano S, Tomokiyo A, Hasegawa D, Yoshida S, Sugii H, Fujino S, Adachi O, Kadowaki M, Yamashita D, Maeda H.  PAX9 Is Involved in Periodontal Ligament Stem Cell-like Differentiation of Human-Induced Pluripotent Stem Cells by Regulating Extracellular Matrix  Biomedicines  2022  10(10):2366  PubMed ID: 36289626   DOI: 10.3390/biomedicines10102366

5611  Wakabayashi H, Narita T, Suga A, Sakagami H.  Hormetic response of cultured normal and tumor cells to 2-aminotropone derivatives.  In Vivo  2010  24(1):39-44  PubMed ID: 20133973