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Cell No. : Cell Name
RCB0156 : 305M  update : 2022/03/07
CommentNormal human skin fibroblast
Comment from the depositor
Terms and conditionsAging reseach group use only.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Ohno, Tadao
Originator Matsumura, Toshiharu
Year of deposit 1987
Animal human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Female
Age at sampling 26 years
Tissue skin
Classification normal
Lifespan finite
Crisis PDL 47 PDL
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_6531
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives HF-RITC80-7 (or MEM ALPHA) + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Virus (HIV) (-)
Isozyme LD, NP, G6PD
Chromosome mode 43-47(50) : 43(1),44(4),45(7),46(36),47(2)
STR(human) OK
Images
deposit info
lot info
Reference information Reference 1
User's Publication 1


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Reference
1495  Ohno T, Yamada M  Growth stimulation by direct cell-cell contact (contact stimulation) in normal human lung fibroblasts.  Biomed Pharmacother  1984  38:337-43  PubMed ID: 6525431  

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User's Publication
5611  Wakabayashi H, Narita T, Suga A, Sakagami H.  Hormetic response of cultured normal and tumor cells to 2-aminotropone derivatives.  In Vivo  2010  24(1):39-44  PubMed ID: 20133973  



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