Cell No. : Cell Name
RCB0156 : 305M update : 2022/12/14 |
||||
Comment | Normal human skin fibroblast | |||
Comment from the depositor | ||||
Terms and conditions | Aging reseach group use only. | |||
Remarks | ||||
Order Form | Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)   | |||
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp). | ||||
Basic information | Depositor | Ohno, Tadao | ||
Originator | Matsumura, Toshiharu | |||
Year of deposit | 1987 | |||
Animal | _human < Mammals | |||
Genus | Homo | |||
Species | sapiens | |||
Race | Japanese | |||
Gender | Female | |||
Age at sampling | 26 years | |||
Tissue | skin | |||
Classification | normal | Lifespan | finite | |
Crisis PDL | 47 PDL | |||
Morphology | fibroblast-like | |||
Cellosaurus(Expasy) | CVCL_6531 | |||
Medium | Medium List | Culture type | Adherent cells | |
Culture medium | RITC80-7 (or MEM ALPHA) + 10% FBS | |||
Antibiotics | Free | Passage method | 0.25% Trypsin | Culture information | Passage ratio | 1 : 8 split |
SC frequency | Subculture : 2 times/week | |||
Temperature | 37 ℃ | |||
CO2 concentration | 5 % | |||
Freeze medium | Medium + 10% DMSO | |||
Freezing method | Slow freezing | |||
Mycoplasma/Acholeplasma | (-) | |||
Virus (HIV) | (-) | |||
Isozyme | LD, NP, G6PD | |||
Chromosome mode | 43-47(50) : 43(1),44(4),45(7),46(36),47(2) | |||
STR(human) | OK | |||
Images | ||||
|
|
|||
Reference information | Reference | 1 | ||
User's Publication | 2 | |||
Reference |
1495 Ohno T, Yamada M Growth stimulation by direct cell-cell contact (contact stimulation) in normal human lung fibroblasts. Biomed Pharmacother 1984 38:337-43 PubMed ID: 6525431 |