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Cell No. : Cell Name
RCB0153 : HUC-F  update : 2022/03/07
CommentNormal umbilical cord fibroblast, female
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Ohno,T. & Nishida,M.
Originator Ohno, Tadao
Year of deposit 1987
Animal human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Female
Tissue umbilical cord
Classification normal
Year of origin 1987
Lifespan finite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_4684
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives HF-RITC80-7 (or MEM ALPHA) + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 4 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Isozyme LD, NP
Chromosome mode 44-47(50) : 44(1),45(1),46(47),47(1)
STR(human) OK
Reference information Reference 1
User's Publication 4


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Reference
11648  Ohno T, Saijo-Kurita K, Miyamoto-Eimori N, Kurose T, Aoki Y, Yosimura S  A simple method for in situ freezing of anchorage-dependent cells including rat liver parenchymal cells.  Cytotechnology  1991  5:273-7  PubMed ID: 1367380  

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User's Publication
6176  Mizugishi K, Yamashita K.  Neutrophil extracellular traps are critical for pregnancy loss in sphingosine kinase-deficient mice on 129Sv/C57BL/6 background.  FASEB J  2017  31(12):5577-5591  PubMed ID: 28842426   DOI: 10.1096/fj.201700399RR
8788  Hiroyama T, Sudo K, Aoki N, Miharada K, Danjo I, Fujioka T, Nagasawa T, Nakamura Y.  Human umbilical cord-derived cells can often serve as feeder cells to maintain primate embryonic stem cells in a state capable of producing hematopoietic cells.  Cell Biol. Int.  2008  32:1-7  PubMed ID: 17890111   DOI: 10.1016/j.cellbi.2007.08.001
3579  Sudo K, Kanno M, Miharada K, Ogawa S, Hiroyama T, Saijo K, Nakamura Y.  Mesenchymal progenitors able to differentiate into osteogenic, chondrogenic, and/or adipogenic cells in vitro are present in most primary fibroblast-like cell populations.  Stem Cells  2007  25(7):1610-7  PubMed ID: 17395773   DOI: 10.1634/stemcells.2006-0504
3514  Katsumata K, Ikeda H, Sato M, Ishizu A, Kawarada Y, Kato H, Wakisaka A, Koike T, Yoshiki T.  Cytokine regulation of env gene expression of human endogenous retrovirus-R in human vascular endothelial cells.  Clin Immunol  1999  93(1):75-80  PubMed ID: 10497013   DOI: 10.1006/clim.1999.4762



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