Cell No. : Cell Name
RCB0153 : HUC-F
update : 2022/03/07
|
| Comment | Normal umbilical cord fibroblast, female |
| Comment from the depositor | |
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
|
|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
|
|
|
Basic information
|
Depositor |
Ohno,T. & Nishida,M.
|
| Originator |
Ohno, Tadao
|
| Year of deposit |
1987
|
| Animal |
_human
< Mammals
|
| Genus |
Homo
|
| Species |
sapiens
|
| Race |
Japanese
|
| Gender |
Female
|
| Tissue |
umbilical cord
|
| Classification |
normal
|
| Year of origin |
1987
|
|
Lifespan |
finite
|
| Morphology |
fibroblast-like
|
| Cellosaurus(Expasy) |
CVCL_4684
|
|
| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
|
Adherent cells
|
| Culture medium |
|
HF-RITC80-7 (or MEM ALPHA) + 10% FBS
|
| Antibiotics |
|
Free
|
|
Passage method |
|
0.25% Trypsin
|
Culture information
|
Passage ratio |
|
1 : 4 split
|
| SC frequency |
|
Subculture : 2 times/week
|
| Temperature |
|
37
℃
|
| CO2 concentration |
|
5
%
|
| Freeze medium |
|
Medium + 10% DMSO
|
| Freezing method |
|
Slow freezing
|
| Mycoplasma/Acholeplasma |
|
(-)
|
| Isozyme |
|
LD, NP
|
| Chromosome mode |
|
44-47(50) : 44(1),45(1),46(47),47(1)
|
| STR(human) |
|
OK
|
| Reference information |
Reference |
2
|
| User's Publication |
5
|
| Reference |
|
893
Sudo K, Kanno M, Miharada K, Ogawa S, Hiroyama T, Saijo K, Nakamura Y.
Mesenchymal progenitors able to differentiate into osteogenic, chondrogenic, and/or adipogenic cells in vitro are present in most primary fibroblast-like cell populations
Stem Cells
2007
25(7):1610-7
PubMed ID: 17395773
DOI: 10.1634/stemcells.2006-0504
|
|
11648
Ohno T, Saijo-Kurita K, Miyamoto-Eimori N, Kurose T, Aoki Y, Yosimura S
A simple method for in situ freezing of anchorage-dependent cells including rat liver parenchymal cells.
Cytotechnology
1991
5:273-7
PubMed ID: 1367380
DOI: 10.1007/BF00556297
|
| User's Publication |
|
6176
Mizugishi K, Yamashita K.
Neutrophil extracellular traps are critical for pregnancy loss in sphingosine kinase-deficient mice on 129Sv/C57BL/6 background.
FASEB J
2017
31(12):5577-5591
PubMed ID: 28842426
DOI: 10.1096/fj.201700399RR
|
|
19135
Yao M, Yao D, Yamaguchi M, Chida J, Yao D, Kido H.
Bezafibrate upregulates carnitine palmitoyltransferase II expression and promotes mitochondrial energy crisis dissipation in fibroblasts of patients with influenza-associated encephalopathy
Mol Genet Metab
2011
104(3):265-72
PubMed ID: 21816645
DOI: 10.1016/j.ymgme.2011.07.009
|
|
8788
Hiroyama T, Sudo K, Aoki N, Miharada K, Danjo I, Fujioka T, Nagasawa T, Nakamura Y.
Human umbilical cord-derived cells can often serve as feeder cells to maintain primate embryonic stem cells in a state capable of producing hematopoietic cells.
Cell Biol. Int.
2008
32:1-7
PubMed ID: 17890111
DOI: 10.1016/j.cellbi.2007.08.001
|
|
3513
Ishimi Y, Okayasu I, Kato C, Kwon HJ, Kimura H, Yamada K, Song SY.
Enhanced expression of Mcm proteins in cancer cells derived from uterine cervix.
Eur J Biochem
2003
270(6):1089-101
PubMed ID: 12631269
DOI: 10.1046/j.1432-1033.2003.03440.x
|
|
3514
Katsumata K, Ikeda H, Sato M, Ishizu A, Kawarada Y, Kato H, Wakisaka A, Koike T, Yoshiki T.
Cytokine regulation of env gene expression of human endogenous retrovirus-R in human vascular endothelial cells.
Clin Immunol
1999
93(1):75-80
PubMed ID: 10497013
DOI: 10.1006/clim.1999.4762
|
Back
Top
CELL BANK Website