Cell No. : Cell Name RCB0153 : HUC-F  update : 2022/03/07
Comment			Normal umbilical cord fibroblast, female
Comment from the depositor
Terms and conditions			There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form			Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)
			Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
	Basic information	Depositor	Ohno,T. & Nishida,M.
		Originator	Ohno, Tadao
		Year of deposit	1987
		Animal	_human < Mammals
		Genus	Homo
		Species	sapiens
		Race	Japanese
		Gender	Female
		Tissue	umbilical cord
		Classification	normal
		Year of origin	1987
		Lifespan	finite
		Morphology	fibroblast-like
		Cellosaurus(Expasy)	CVCL_4684
			deposit info	lot info
		Medium	Medium List
		Culture type		Adherent cells
		Culture medium		HF-RITC80-7 (or MEM ALPHA) + 10% FBS
		Antibiotics		Free
		Passage method		0.25% Trypsin
	Culture information	Passage ratio		1 : 4 split
		SC frequency		Subculture : 2 times/week
		Temperature		37 ℃
		CO2 concentration		5 %
		Freeze medium		Medium + 10% DMSO
		Freezing method		Slow freezing
		Mycoplasma		(-)
		Isozyme		LD, NP
		Chromosome mode		44-47(50) : 44(1),45(1),46(47),47(1)
		STR(human)		OK
Reference information		Reference	2
		User's Publication	4

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Reference

893  Sudo K, Kanno M, Miharada K, Ogawa S, Hiroyama T, Saijo K, Nakamura Y.  Mesenchymal progenitors able to differentiate into osteogenic, chondrogenic, and/or adipogenic cells in vitro are present in most primary fibroblast-like cell populations  Stem Cells  2007  25(7):1610-7  PubMed ID: 17395773   DOI: 10.1634/stemcells.2006-0504

11648  Ohno T, Saijo-Kurita K, Miyamoto-Eimori N, Kurose T, Aoki Y, Yosimura S  A simple method for in situ freezing of anchorage-dependent cells including rat liver parenchymal cells.  Cytotechnology  1991  5:273-7  PubMed ID: 1367380   DOI: 10.1007/BF00556297

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User's Publication

6176  Mizugishi K, Yamashita K.  Neutrophil extracellular traps are critical for pregnancy loss in sphingosine kinase-deficient mice on 129Sv/C57BL/6 background.  FASEB J  2017  31(12):5577-5591  PubMed ID: 28842426   DOI: 10.1096/fj.201700399RR

8788  Hiroyama T, Sudo K, Aoki N, Miharada K, Danjo I, Fujioka T, Nagasawa T, Nakamura Y.  Human umbilical cord-derived cells can often serve as feeder cells to maintain primate embryonic stem cells in a state capable of producing hematopoietic cells.  Cell Biol. Int.  2008  32:1-7  PubMed ID: 17890111   DOI: 10.1016/j.cellbi.2007.08.001

3513  Ishimi Y, Okayasu I, Kato C, Kwon HJ, Kimura H, Yamada K, Song SY.  Enhanced expression of Mcm proteins in cancer cells derived from uterine cervix.  Eur J Biochem  2003  270(6):1089-101  PubMed ID: 12631269   DOI: 10.1046/j.1432-1033.2003.03440.x

3514  Katsumata K, Ikeda H, Sato M, Ishizu A, Kawarada Y, Kato H, Wakisaka A, Koike T, Yoshiki T.  Cytokine regulation of env gene expression of human endogenous retrovirus-R in human vascular endothelial cells.  Clin Immunol  1999  93(1):75-80  PubMed ID: 10497013   DOI: 10.1006/clim.1999.4762