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Cell No. : Cell Name
RCB0146 : P3-X63-Ag8.653  update : 2020/09/24
CommentMyeloma
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, a prior written permission of the DEPOSITOR or the RIKEN BRC is necessary.
Remarks
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
Basic information Depositor Murakami, Yasufumi
Originator Kearney, J.
Year of deposit 1987
Animal mouse < Mammals
Tissue myeloma
Case history myeloma
Classification cancer
Lifespan infinite
Morphology lymphocyte-like
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives RPMI1640 + 10% FBS
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 10 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
Chromosome mode 50-54(50) : 50(1),52(3),53(20),54(26)
Images
deposit info
lot info
Reference information Reference 2
User's Publication 4


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Reference
951  Kearney, J F, Radbruch A, Liesegang B, Rajewsky K  A new mouse myeloma cell line that has lost immunoglobulin expression but permits the construction of antibody-secreting hybrid cell lines.  J Immunol  1979  123:1548-50  PubMed ID: 113458
2607  Horibata K, Harris, A W  Mouse myelomas and lymphomas in culture.  Exp Cell Res  1970  60:61-77  PubMed ID: 5439579

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User's Publication
7222  Itagaki T, Arima Y, Kuwabara R, Kitamura N, Iwata H.  Interaction between cells and poly(ethylene glycol)-lipid conjugates.  Colloids Surf B Biointerfaces  2015  135:765-73  PubMed ID: 26342322
7461  Ukaji T, Hashimoto M, Kai O.  Conditioned medium from gerbil--mouse T cell heterohybridomas improved antibody secretion.  Exp. Anim.  2015  64:199-205  PubMed ID: 25739359
7687  Ukaji T, Iwasa MA, Kai O.  Establishment of gerbil-mouse heterohybridoma secreting immunoglobulin of Mongolian gerbil (Meriones unguiculatus) establishment of gerbil-mouse heterohybridoma.  J. Immunol. Methods  2011  373:174-80  PubMed ID: 21907207
5421  Miyamoto T, Tian H, Matsuno K, Takata R, Hatano S.  Application of Monoclonal Antibodies to Dulcitol 1-Phosphate Dehydrogenase for Rapid Detection of Salmonella.  J Food Prot  1995  58(8):847-852  PubMed ID: 31137399



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