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Cell No. : Cell Name
RCB0146 : P3-X63-Ag8.653  update : 2020/09/24
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, a prior written permission of the DEPOSITOR or the RIKEN BRC is necessary.
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC( regarding any kind of for-profit use.
Basic information Depositor Murakami, Yasufumi
Originator Kearney, J.
Year of deposit 1987
Animal mouse < Mammals
Tissue myeloma
Case history myeloma
Classification cancer
Lifespan infinite
Morphology lymphocyte-like
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives RPMI1640 + 10% FBS
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 10 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
Chromosome mode 50-54(50) : 50(1),52(3),53(20),54(26)
deposit info
lot info
Reference information Reference 2
User's Publication 4

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951  Kearney, J F, Radbruch A, Liesegang B, Rajewsky K  A new mouse myeloma cell line that has lost immunoglobulin expression but permits the construction of antibody-secreting hybrid cell lines.  J Immunol  1979  123:1548-50  PubMed ID: 113458
2607  Horibata K, Harris, A W  Mouse myelomas and lymphomas in culture.  Exp Cell Res  1970  60:61-77  PubMed ID: 5439579

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User's Publication
7222  Itagaki T, Arima Y, Kuwabara R, Kitamura N, Iwata H.  Interaction between cells and poly(ethylene glycol)-lipid conjugates.  Colloids Surf B Biointerfaces  2015  135:765-73  PubMed ID: 26342322
7461  Ukaji T, Hashimoto M, Kai O.  Conditioned medium from gerbil--mouse T cell heterohybridomas improved antibody secretion.  Exp. Anim.  2015  64:199-205  PubMed ID: 25739359
7687  Ukaji T, Iwasa MA, Kai O.  Establishment of gerbil-mouse heterohybridoma secreting immunoglobulin of Mongolian gerbil (Meriones unguiculatus) establishment of gerbil-mouse heterohybridoma.  J. Immunol. Methods  2011  373:174-80  PubMed ID: 21907207
5421  Miyamoto T, Tian H, Matsuno K, Takata R, Hatano S.  Application of Monoclonal Antibodies to Dulcitol 1-Phosphate Dehydrogenase for Rapid Detection of Salmonella.  J Food Prot  1995  58(8):847-852  PubMed ID: 31137399

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