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Cell No. : Cell Name
RCB0142 : Ehrlich  update : 2021/02/26
CommentEhrlich's ascite tumor
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Kurita, Kaoru
Year of deposit 1987
Animal _mouse < Mammals
Tissue ascites
Disease name ascite tumor
Classification cancer
History Maintained in vivo.
Lifespan infinite
Morphology lymphocyte-like
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives DMEM (low glucose) + 10% FBS
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 10 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
deposit info
lot info
Reference information Reference 2
User's Publication 6

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4611  Boone C, Sasaki M, McKee W.  Characterization of an in vitro strain of Ehrlich-Lettre ascites carcinoma subjected to many periodic mouse passages.  J Natl Cancer Inst  1965  34(6):725-40  PubMed ID: 5829538
4612  SATO J, MATSUDA S, YABE T, USUI K, NODA M.  Tissue culture of Ehrlich ascites tumor cells.  Bull Cancer Inst Okayama Univ Med Sch  1961  1:42-69  PubMed ID: 14497414

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User's Publication
13405  Kato S, Saitoh Y, Miwa N.  Carcinostatic effects of alkanoyl ascorbate plus platinum nano-colloid and stabilization of the esterolytically resultant ascorbate by hydrogen.  Hum Cell  2021    PubMed ID: 33387361
13284  Kamimura M, Sasaki A, Watanabe S, Tanaka S, Fukukawa A, Takeda K, Nakamura Y, Nakamura T, Kuramochi K, Otani Y, Hashimoto F, Ishimaru K, Matsuo T, Okamoto S.  Chemical and molecular bases of dome formation in human colorectal cancer cells mediated by sulphur compounds from Cucumis melo var. conomon.  FEBS Open Bio  2020    PubMed ID: 33048473
11603  Aoki N, Matsui Y.  Comprehensive Analysis of Mouse Cancer/Testis Antigen Functions in Cancer Cells and Roles of TEKT5 in Cancer Cells and Testicular Germ Cells.  Mol. Cell. Biol.  2019    PubMed ID: 31208979
12862  Saitoh Y, Ikeshima M, Kawasaki N, Masumoto A, Miwa N.  Transient generation of hydrogen peroxide is responsible for carcinostatic effects of hydrogen combined with platinum nanocolloid, together with increases intracellular ROS, DNA cleavages, and proportion of G2/M-phase  Free Radic Res  2016  50(4):385-95  PubMed ID: 26652652
7298  Saitoh Y, Yoshimoto T, Kato S, Miwa N.  Synergic carcinostatic effects of ascorbic acid and hyperthermia on Ehrlich ascites tumor cell.  Exp. Oncol.  2015  37:94-9  PubMed ID: 26112934
1399  Eki T, Enomoto T, Masutani C, Miyajima A, Takada R, Murakami Y, Ohno T, Hanaoka F, Ui M  Mouse DNA primase plays the principal role in determination of permissiveness for polyomavirus DNA replication.  J Virol  1991  65:4874-81  PubMed ID: 1651410

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