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Cell No. : Cell Name
RCB0137 : M10^(-)  update : 2022/03/07
CommentResistant to 6TG, isolated from M10
Comment from the depositor
Terms and conditionsA prior written permission of the approver(Depositor) is necessary for any kind of use including academic use and for-profit use.
Remarks
approver's address
×
English
Address
Nihon University
College of Bioresource Sciences Department of Veterinary Medicine
1866, Kameino, Fujisawa-shi, Kanagawa-Ken, 252-8510 ,Japan
Dr.KOSAKA Toshifumi
Fax. +81-466-82-1310
Order Form Order Form(C-0005.pdf)   Approval Form(C-0006.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Kosaka, Toshifumi
Originator Kosaka, Toshifumi
Year of deposit 1987
Original cell L5178Y
Animal mouse < Mammals
Tissue lymph node
Classification cancer
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_L285
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives RPMI1640 + 10% FBS
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 10 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
Reference information Reference 2
User's Publication 0


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Reference
2626  Sato K, Hieda-Shiomi N, Hama-Inaba H  X-ray-sensitive mutant mouse cells with various sensitivities to chemical mutagens.  Mutat Res  1983  121:281-5  PubMed ID: 6413855  
2625  Sato K, Hieda N  Isolation and characterization of a mutant mouse lymphoma cell sensitive to methyl methanesulfonate and X rays.  Radiat Res  1979  78:167-71  PubMed ID: 221956  

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