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Cell No. : Cell Name
RCB0136 : M10  update : 2022/03/08
CommentHighly sensitive to X-ray irradiation
Comment from the depositor
Terms and conditionsA prior written permission of the approver(Depositor) is necessary for any kind of use including academic use and for-profit use.
Remarks
approver's address
×
English
Address
RIKEN BioResource Research Center,
Cell Bank, 3-1-1 Koyadai,Tsukuba-shi, Ibaraki 305-0074 Japan
Dr.SATO Koki
Fax. +81-29-836-9130
Order Form Order Form(C-0005.pdf)   Approval Form(C-0006.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Sato, Koki
Originator Sato, Koki
Year of deposit 1987
Original cell L5178Y
Animal mouse < Mammals
Tissue lymph node
Classification cancer
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_L284
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives RPMI1640 + 10% FBS
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 10 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
Reference information Reference 2
User's Publication 7


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Reference
2626  Sato K, Hieda-Shiomi N, Hama-Inaba H  X-ray-sensitive mutant mouse cells with various sensitivities to chemical mutagens.  Mutat Res  1983  121:281-5  PubMed ID: 6413855
2625  Sato K, Hieda N  Isolation and characterization of a mutant mouse lymphoma cell sensitive to methyl methanesulfonate and X rays.  Radiat Res  1979  78:167-71  PubMed ID: 221956

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User's Publication
20422  Asa ADDC, Wanotayan R, Sharma MK, Tsukada K, Shimada M, Matsumoto Y.  Functional analysis of XRCC4 mutations in reported microcephaly and growth defect patients in terms of radiosensitivity.  J Radiat Res  2021  62(3):380-389  PubMed ID: 33842963
10650  Sunatani Y, Kamdar RP, Sharma MK, Matsui T, Sakasai R, Hashimoto M, Ishigaki Y, Matsumoto Y, Iwabuchi K.  Caspase-mediated cleavage of X-ray repair cross-complementing group 4 promotes apoptosis by enhancing nuclear translocation of caspase-activated DNase.  Exp. Cell Res.  2018  362:450-460  PubMed ID: 29233683
11216  Amiri Moghani AR, Sharma MK, Matsumoto Y.  In cellulo phosphorylation of DNA double-strand break repair protein XRCC4 on Ser260 by DNA-PK.  J. Radiat. Res.  2018    PubMed ID: 30247612
7365  Fukuchi M, Wanotayan R, Liu S, Imamichi S, Sharma MK, Matsumoto Y.  Lysine 271 but not lysine 210 of XRCC4 is required for the nuclear localization of XRCC4 and DNA ligase IV.  Biochem. Biophys. Res. Commun.  2015  461:687-94  PubMed ID: 25934149
7508  Wanotayan R, Fukuchi M, Imamichi S, Sharma MK, Matsumoto Y.  Asparagine 326 in the extremely C-terminal region of XRCC4 is essential for the cell survival after irradiation.  Biochem. Biophys. Res. Commun.  2015  457:526-31  PubMed ID: 25597996
4525  Shoji Imamichi, Mukesh Kumar Sharma, Radhika Pankaj Kamdar, Mikoto Fukuchi, Yoshihisa Matsumoto  Ionizing radiation-induced XRCC4 phosphorylation is mediated through ATM in addition to DNA-PK  Proc Jpn Acad Ser B Phys Biol Sci  2014  90(9):365-72  PubMed ID: 25391321
5901  Masanobu Kawanishi, Katsuyuki Okuyama, Kazunori Shiraishi, Yatsuka Matsuda, Ryoichi Taniguchi, Nobuyuki Shiomi, Morio Yonezawa, Takashi Yagi  Growth retardation of Paramecium and mouse cells by shielding them from background radiation  J Radiat Res  2012  53(3):404-10  PubMed ID: 22739010



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