細胞番号 : 細胞名 RCB0135 : L5178Y  update : 2024/01/17
細胞特性(Comment:英)			Rapidly growing lymphoma
細胞特性(日)			非常に早く増殖し軟寒天中でのコロニー形成率も良いため、放射線生物学や変異株分離の研究に多用される。
細胞特性(寄託者記述:英)
細胞特性(寄託者記述:日)
使用条件(英)			There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
使用条件(日)			基礎的研究につきましては、使用制限はありません。商業利用や特許取得を目的とする場合は、事前に必ず理研細胞バンクに連絡をすること。
備考(英)
備考(日)
提供申込書類(英)			Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)
			Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
提供申込書類(日)			依頼書C-0001.pdf   同意書(非営利学術目的)C-0003.pdf   同意書(営利目的)C-0003p.pdf
			提供同意書は、使用機関の種類や目的に応じて、非営利学術目的 (C-XXXX) と営利目的 (C-XXXXp) の2種類があります。該当する提供同意書をご使用ください（詳細）。特許等の取得及び商業利用等は事前に必ず cellbank.brc@riken.jp までご連絡ください。
提供手数料			手数料とお支払いについてはこちらをご覧ください。
細胞基本情報		寄託者	Kaneko, Ichiro
		寄託日	1987
		動物種	_mouse < Mammals
		採取組織	lymph node
		病名	lymphoma
		細胞分類	other
		入手歴	RIKEN.-K.Kaneko
		細胞寿命	infinite
		細胞形態	lymphocyte-like
		Cellosaurus(Expasy)	CVCL_2097
細胞培養・検査情報			寄託時情報	ロット情報
	培地・試薬情報		培地・試薬一覧はこちらをご覧ください。
		培養形態		Suspension cells
		培養方法		General Guidelines for Culturing Suspension Cells, 浮遊細胞の培養に関する一般的な注意(Japanese)
		培地		RPMI1640 + 10% FBS
		抗生物質		Free
		継代方法		dilution
		継代密度		1 : 10 split
		継代・培地交換頻度		Subculture : 2 times/week
		培養最適温度		37 ℃
		二酸化炭素濃度		5 %
		凍結培地		Medium + 10% DMSO
		凍結方法		Slow freezing
		マイコプラズマ/アコレプラズマ		(-)
		マウス系統検査		OK
		アイソザイム検査		LD, NP
		染色体検査		32-79(50) : /36(2),37(5),38(10),39(6),40(3),42(5)/
文献情報		Reference(英)	13件
		Reference(日)	7件
		利用者成果(英)	13件
		利用者成果(日)	0件

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Reference(英)

2617  Applegate, M L, Moore, M M, Broder, C B, Burrell A, Juhn G, Kasweck, K L, Lin, P F, Wadhams A, Hozier, J C  Molecular dissection of mutations at the heterozygous thymidine kinase locus in mouse lymphoma cells.  Proc Natl Acad Sci U S A  1990  87:51-5  PubMed ID: 1967496   DOI: 10.1073/pnas.87.1.51

2616  Chen, L P, Suzuki Y, Liu, C M, Wheelock, E F  Maintenance and cure of the L5178Y murine tumor-dormant state by interleukin 2: dependence of interleukin 2 on induced interferon-gamma and on tumor necrosis factor for its antitumor effects.  Cancer Res  1990  50:1368-74  PubMed ID: 2105837

2619  Doerr, C L, Harrington-Brock K, Moore, M M  Micronucleus, chromosome aberration, and small-colony TK mutant analysis to quantitate chromosomal damage in L5178Y mouse lymphoma cells.  Mutat Res  1989  222:191-203  PubMed ID: 2466200   DOI: 10.1016/0165-1218(89)90135-3

2618  Furuno-Fukushi I, Ueno, A M, Matsudaira H  Mutation induction by very low dose rate gamma rays in cultured mouse leukemia cells L5178Y.  Radiat Res  1988  115:273-80  PubMed ID: 3406368

2624  Hama-Inaba H, Hieda-Shiomi N, Shiomi T, Sato K  Isolation and characterization of mitomycin-C-sensitive mouse lymphoma cell mutants.  Mutat Res  1983  108:405-16  PubMed ID: 6403846   DOI: 10.1016/0027-5107(83)90136-7

2626  Sato K, Hieda-Shiomi N, Hama-Inaba H  X-ray-sensitive mutant mouse cells with various sensitivities to chemical mutagens.  Mutat Res  1983  121:281-5  PubMed ID: 6413855   DOI: 10.1016/0165-7992(83)90215-4

2622  Shiomi T, Hieda-Shiomi N, Sato K  Isolation of UV-sensitive mutants of mouse L5178Y cells by a cell suspension spotting method.  Somatic Cell Genet  1982  8:329-345  PubMed ID: 7112349   DOI: 10.1007/BF01538891

2623  Shiomi T, Hieda-Shiomi N, Sato K  A novel mutant of mouse lymphoma cells sensitive to alkylating agents and caffeine.  Mutat Res  1982  103:61-9  PubMed ID: 7057785   DOI: 10.1016/0165-7992(82)90088-4

2621  Sato K, Hieda N  Isolation of a mammalian cell mutant sensitive to 4-nitroquinoline-1-oxide.  Int J Radiat Biol Relat Stud Phys Chem Med  1979  35:83-7  PubMed ID: 109406   DOI: 10.1080/09553007914550071

2625  Sato K, Hieda N  Isolation and characterization of a mutant mouse lymphoma cell sensitive to methyl methanesulfonate and X rays.  Radiat Res  1979  78:167-71  PubMed ID: 221956

238  Suzuki N, Okada S  Isolation of nutrient deficient mutants and quantitative mutation assay by reversion of alanine-requiring L5178Y cells.  Mutat Res  1976  34:489-505  PubMed ID: 817197   DOI: 10.1016/0027-5107(76)90225-6

2620  Alexander, P.  Mouse lymphoma cells with different radiosensitivities.  Nature  1961  192:572-573  PubMed ID: 13860357   DOI: 10.1038/192572a0

237  FISCHER, G A, WELCH, A D  Effect of citrovorum factor and peptones on mouse leukemia cells L-5178 in tissue culture.  Science  1957  126:1018-9  PubMed ID: 13486055   DOI: 10.1126/science.126.3281.1018

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Reference(日)

2627  佐藤弘毅  マウスリンパ腫：L5178Y  生体の科学  1992  43:477

1270  佐藤弘毅  バイオテクノロジ－素材としての培養細胞 ＤＮＡ修復  蛋白質 核酸 酵素  1989  34:1388-1398

1226  佐藤弘毅  バイオテクノロジ－素材としての培養細胞 体細胞遺伝学  蛋白質 核酸 酵素  1988  33:289-290

1246  中村典  バイオテクノロジ－素材としての培養細胞 細胞周期  蛋白質 核酸 酵素  1988  33:1328-1329

1253  中村典  バイオテクノロジ－素材としての培養細胞 突然変異・細胞毒性アッセイ  蛋白質 核酸 酵素  1988  33:1827-1828

1232  Suzuki H  バイオテクノロジ－素材としての培養細胞 ホルモン・生理活性物質  蛋白質 核酸 酵素  1988  33:1150-1151

239  Okada, H. et al  L5178Y株；マウス淋巴性白血病細胞  組織培養  1980  6:219-223

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利用者成果(英)

11216  Amiri Moghani AR, Sharma MK, Matsumoto Y.  In cellulo phosphorylation of DNA double-strand break repair protein XRCC4 on Ser260 by DNA-PK.  J. Radiat. Res.  2018    PubMed ID: 30247612   DOI: 10.1093/jrr/rry072

6251  Hye-Sook Chang , Daiji Endoh, Yushi Ishida, Hiroyuki Takahashi, Shuji Ozawa, Masanobu Hayashi, Akira Yabuki, Osamu Yamato  Radioprotective effect of alk(en)yl thiosulfates derived from allium vegetables against DNA damage caused by X-ray irradiation in cultured cells: antiradiation potential of onions and garlic  ScientificWorldJournal  2012  846750  PubMed ID: 22919357   DOI: 10.1100/2012/846750

5901  Masanobu Kawanishi, Katsuyuki Okuyama, Kazunori Shiraishi, Yatsuka Matsuda, Ryoichi Taniguchi, Nobuyuki Shiomi, Morio Yonezawa, Takashi Yagi  Growth retardation of Paramecium and mouse cells by shielding them from background radiation  J Radiat Res  2012  53(3):404-10  PubMed ID: 22739010   DOI: 10.1269/jrr.11145

3032  Wada, Seiichi, Kurahayashi, Hidemitsu, Kobayashi, Yasuhiko, Funayama, Tomoo, Yamamoto, Kazuo, Natsuhori, Masahiro, Ito, Nobuhiko  The relationship between cellular radiosensitivity and radiation-induced DNA damage measured by the comet assay.  J Vet Med Sci  2003  65:471-7  PubMed ID: 12736429   DOI: 10.1292/jvms.65.471

3031  Wada, Seiichi, Khoa, Tran Van, Kobayashi, Yasuhiko, Funayama, Tomoo, Yamamoto, Kazuo, Natsuhori, Masahiro, Ito, Nobuhiko  Detection of radiation-induced apoptosis using the comet assay.  J Vet Med Sci  2003  65:1161-6  PubMed ID: 14665742   DOI: 10.1292/jvms.65.1161

4076  Nonaka M, Ikeda H, Inokuchi T.  Effect of combined photodynamic and chemotherapeutic treatment on lymphoma cells in vitro  Cancer Lett  2002  184(2):171-8  PubMed ID: 12127689   DOI: 10.1016/s0304-3835(02)00208-2

2380  Yokota S, Yanagi H, Yura T, Kubota H  Cytosolic chaperonin is up-regulated during cell growth. Preferential expression and binding to tubulin at G(1)/S transition through early S phase.  J Biol Chem  1999  274:37070-8  PubMed ID: 10601265   DOI: 10.1074/jbc.274.52.37070

4067  TAKAHIRO SUZUKI TORU EZURE MASARU ISHIDA  In‐vitro Antitumour Activity of Extracts From Cyanobacteria  Pharmacy and Pharmacology Communications  1999  5:619-622    DOI: 10.1211/146080899128734244

2359  Suzuki T, Ezure T, Ishida M  Synergistic effects of some pairs of antioxidants and related agents on mouse leukaemia L5178Y cell growth in-vitro  J Pharm Pharmacol  1998  50:1173-1177  PubMed ID: 9821666   DOI: 10.1111/j.2042-7158.1998.tb03330.x

1944  Suzuki, T., and Ishida, M.  Application of Neural Networks in Quantitative Structure-activity Relationships of Cytotoxicity of Antioxidants  Pharmaceutical Sciences  1995  1:297-300

5411  Nakamura M, Sakanaka C, Aoki Y, Ogasawara H, Tsuji T, Kodama H, Matsumoto T, Shimizu T, Noma M.  Identification of two isoforms of mouse neuropeptide Y-Y1 receptor generated by alternative splicing. Isolation, genomic structure, and functional expression of the receptors.  J Biol Chem  1995  270(50):30102-10  PubMed ID: 8530415   DOI: 10.1074/jbc.270.50.30102

1850  Suzuki Takahiro, Ohtaguchi Kazuhisa and Koide Kozo  Modelling the cytotoxicity of antioxidants to mouse leukaemia L5178Y cells in vitro  Pharmaceutical Science Communications  1994  4:171-181

1772  Suzuki, T., Ohtaguchi, K. and Koide, K.  Cell Growth Inhibition in Mouse Leukemia L5178Y Cells In Vitro by Crude Extracts from Freshwater Blue-Green Algae  J Chemical Engineering  1993  26:463-464    DOI: 10.1252/jcej.26.463

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利用者成果(日)