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Cell No. : Cell Name
RCB0120 : LT4Tr  update : 2022/03/07
CommentSpontaneous transformant of LT4
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Koyasu, Shigeo
Originator Koyasu, Shigeo
Year of deposit 1986
Animal _mouse < Mammals
Gender Female
Tissue spleen
Classification mutant
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_T728
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Culture medium RPMI1640 + 10% FBS + 50μM 2-Mercaptoethanol
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 10 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
Chromosome mode 56-75(50) : /66(5),67(7),68(5),69(10),70(2),71(8)/
deposit info
lot info
Reference information Reference 1
User's Publication 3

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1014  Koyasu S, Yodoi J, Nikaido T, Tagaya Y, Taniguchi Y, Honjo T, Yahara I  Expression of interleukin 2 receptors on interleukin 3-dependent cell lines.  J Immunol  1986  136:984-7  PubMed ID: 3079807  

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User's Publication
7045  Kazuhisa FujimotoTakuya MiyataYasuhiro Aoyama  Saccharide-Directed Cell Recognition and Molecular Delivery Using Macrocyclic Saccharide Clusters:  Masking of Hydrophobicity to Enhance the Saccharide Specificity  J Am Chem Soc.   2000  122:3558-3559    DOI: 10.1021/ja993993d
3930  Nemoto S, Otsuka M, Arakawa N.  Inhibitory effect of ascorbate on cell growth: relation to catalase activity.  J Nutr Sci Vitaminol (Tokyo).  1996  42(2):77-85  PubMed ID: 8780966   DOI: 10.3177/jnsv.42.77
7049  Nemoto S, Otsuka M, Arakawa N.  A positive correlation between catalase activity and ascorbate uptake in the tissues of guinea pigs and cultured cells of mammals.  J Nutr Sci Vitaminol (Tokyo)  1996  42(5):387-95  PubMed ID: 8981246   DOI: 10.3177/jnsv.42.387

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