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Cell No. : Cell Name
RCB0116 : 7501  update : 2022/03/07
CommentS. cerevisiae Endo.Sce I large subunit mAb.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Makino, Osamu
Originator Nakagawa, Kei-ich et al.
Year of deposit 1986
Animal mouse x mouse < Hybrid
Tissue hybridoma
Classification hybrid cells
Memo_1 Mycoplasma was eliminated with MC-210.
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_B991
hybridoma Fusion_cell_1 strain mouse, BALB/c
Fusion_cell_2 name P3-X63-Ag8.653
Fusion_cell_2 strain mouse
Fusion method HAT selection
Antibody name 7501
Subclass IgG2b
Immunogen Saccharomyces cerevisiae, Endo.Sce I
Antigen_name Endo.Sce I large
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives RPMI1640 + 10% FBS
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 10 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Antibody subclass IgG2b
Reference information Reference 1
User's Publication 0


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Reference
1063  Nakagawa K, Hashikawa J, Makino O, Ando T, Shibata T.  Subunit structure of a yeast site-specific endodeoxyribonuclease, endo SceI. A study using monoclonal antibodies.  Eur J Biochem  1988  171(1-2):23-9  PubMed ID: 2828049   DOI: 10.1111/j.1432-1033.1988.tb13753.x

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