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Cell No. : Cell Name
RCB0115 : ARM321  update : 2022/03/07
CommentAnti-recA protein mAb.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Shibata, Takehiko
Originator Makino, Osamu et al.
Year of deposit 1986
Animal mouse x mouse < Hybrid
Tissue hybridoma
Classification hybrid cells
Memo_1 Mycoplasma was eliminated with MC-210.
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_B993
hybridoma Fusion_cell_1 strain mouse, BALB/c
Fusion_cell_2 name P3-X63-Ag8.653
Fusion_cell_2 strain mouse
Fusion method HAT selection
Antibody name ARM321
Subclass IgG1
Antigen_name recA protein
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives RPMI1640 + 10% FBS
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 10 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Antibody subclass IgG1
Images
deposit info
lot info
Reference information Reference 4
User's Publication 0


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Reference
1298  Ikeda M, Makino O, Shibata T  Epitopes and active sites of the RecA protein.  J Biol Chem  1990  265:8948-56  PubMed ID: 1692839
1297  Ikeda M, Makino O, Shibata T  Probing the activation stages of the RecA protein by monoclonal IgGs during the pairing of homologous DNA molecules.  J Biol Chem  1990  265:8957-65  PubMed ID: 2140364
1065  Makino O, Ikawa S, Shibata Y, Maeda H, Ando T, Shibata T  rec-A protein-promoted recombination reaction consists of two independent processes, homologous matching and processive unwinding. A study involving an anti-rec-A protein-monoclonal IgG.  J Biol Chem  1987  262:12237-46  PubMed ID: 3624254
1064  Makino O, Shibata Y, Maeda H, Shibata T, Ando T  Monoclonal antibodies with specific effects on partial activities of recA protein of Escherichia coli.  J Biol Chem  1985  260:15402-5  PubMed ID: 2933399

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