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細胞番号 : 細胞名
RCB0112 : NRK49F  update : 2022/03/07
細胞特性(Comment:英)Contact-inhibited semi-normal cell line
使用条件(英)There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
提供申込書類(英) Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
提供申込書類(日) 依頼書C-0001.pdf   同意書(非営利学術目的)C-0003.pdf   同意書(営利目的)C-0003p.pdf  
提供同意書は、使用機関の種類や目的に応じて、非営利学術目的 (C-XXXX) と営利目的 (C-XXXXp) の2種類があります。該当する提供同意書をご使用ください(詳細)。特許等の取得及び商業利用等は事前に必ず cellbank.brc@riken.jp までご連絡ください。
提供手数料 手数料とお支払いについてはこちらをご覧ください。
細胞基本情報 寄託者 Ohno, Tadao
樹立者 Delarco, J. E.
寄託日 1986
動物種 rat < Mammals
属名 Rattus
種名 norvegicus
採取組織 kidney
細胞分類 semi-normal
細胞寿命 infinite
細胞形態 fibroblast-like
Cellosaurus(Expasy) CVCL_2144
培地・試薬情報 培地・試薬一覧はこちらをご覧ください。
培養形態 Adherent cells
培地 MEM + 5% CS
抗生物質 Free
継代方法 0.25% Trypsin
継代密度 1 : 8 split
継代・培地交換頻度 Subculture : once/week, Medium Renewal : 2 times/week
培養最適温度 37 ℃
二酸化炭素濃度 5 %
凍結培地 Medium + 10% DMSO
凍結方法 Slow freezing
マイコプラズマ (-)
アイソザイム検査 LD, NP
染色体検査 38-42(50) : 38(1),39(2),40(1),41(45),42(1)
文献情報 Reference(英) 4件
Reference(日) 3件
利用者成果(英) 11件
利用者成果(日) 1件

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15439  Shams R, Matsukawa A, Ochi Y, Ito Y, Miyatake H.  In Silico and In Cell Hybrid Selection of Nonrapalog Ligands to Allosterically Inhibit the Kinase Activity of mTORC1  J Med Chem  2022  Jun 30  PubMed ID: 34191518
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2757  伊奈啓輔、北村裕和、立川修二、宮崎孝志、阿部博和、藤倉義久  線維芽細胞の収縮機構  日本解剖学会 第60回九州支部学術集会  2004  12 

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