細胞番号 : 細胞名
RCB0112 : NRK49F
update : 2024/06/12
|
| 細胞特性(Comment:英) | Contact-inhibited semi-normal cell line |
| 細胞特性(日) | 接触阻止能を示すラット腎細胞NRKのクローンの一つである。TGFαとTGFβにより軟寒天培地中でコロニーを形成するようになる。 |
| 細胞特性(寄託者記述:英) | |
| 細胞特性(寄託者記述:日) | |
| 使用条件(英) | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| 使用条件(日) | 基礎的研究につきましては、使用制限はありません。商業利用や特許取得を目的とする場合は、事前に必ず理研細胞バンクに連絡をすること。 |
| 備考(英) | |
| 備考(日) | |
| 提供申込書類(英) |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
|
|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
|
| 提供申込書類(日) |
依頼書C-0001.pdf
同意書(非営利学術目的)C-0003.pdf
同意書(営利目的)C-0003p.pdf
|
|
提供同意書は、使用機関の種類や目的に応じて、非営利学術目的 (C-XXXX) と営利目的 (C-XXXXp) の2種類があります。該当する提供同意書をご使用ください（詳細）。特許等の取得及び商業利用等は事前に必ず cellbank.brc@riken.jp までご連絡ください。
|
| 提供手数料 |
手数料とお支払いについてはこちらをご覧ください。 |
|
細胞基本情報
|
|
寄託者 |
Ohno, Tadao
|
| 樹立者 |
Delarco, J. E.
|
| 寄託日 |
1986
|
| 動物種 |
_rat
< Mammals
|
| 属名 |
Rattus
|
| 種名 |
norvegicus
|
| 齢 |
adult
|
| 採取組織 |
kidney
|
| 細胞分類 |
semi-normal
|
|
細胞寿命 |
infinite
|
| 細胞形態 |
fibroblast-like
|
| Cellosaurus(Expasy) |
CVCL_2144
|
|
細胞培養・検査情報
| |
寄託時情報 |
ロット情報 |
| 培地・試薬情報 |
培地・試薬一覧はこちらをご覧ください。 |
|
培養形態 |
|
Adherent cells
|
| 培地 |
|
MEM + 5% CS
|
| 抗生物質 |
|
Free
|
|
継代方法 |
|
0.25% Trypsin
|
|
継代密度 |
|
1 : 8 split
|
| 継代・培地交換頻度 |
|
Subculture : once/week, Medium Renewal : 2 times/week
|
| 培養最適温度 |
|
37
℃
|
| 二酸化炭素濃度 |
|
5
%
|
| 凍結培地 |
|
Medium + 10% DMSO
|
| 凍結方法 |
|
Slow freezing
|
| マイコプラズマ/アコレプラズマ |
|
(-)
|
| 動物種PCR |
|
OK
|
| アイソザイム検査 |
|
LD, NP
|
| 染色体検査 |
|
38-42(50) : 38(1),39(2),40(1),41(45),42(1)
|
| 画像情報 |
寄託時情報 | ロット情報 |
|
|
|
| 文献情報 |
Reference(英) |
4件
|
| Reference(日) |
3件
|
| 利用者成果(英) |
16件
|
| 利用者成果(日) |
0件
|
| 利用者成果(英) |
|
21962
Okamoto Y, Kitakaze K, Takenouchi Y, Matsui R, Koga D, Miyashima R, Ishimaru H, Tsuboi K.
GPR176 promotes fibroblast-to-myofibroblast transition in organ fibrosis progression.
Biochim Biophys Acta Mol Cell Res
2024
1871(7):119798
PubMed ID: 39047914
DOI: 10.1016/j.bbamcr.2024.119798
|
|
15439
Shams R, Matsukawa A, Ochi Y, Ito Y, Miyatake H.
In Silico and In Cell Hybrid Selection of Nonrapalog Ligands to Allosterically Inhibit the Kinase Activity of mTORC1
J Med Chem
2022
Jun 30
PubMed ID: 34191518
DOI: 10.1021/acs.jmedchem.1c00536
|
|
13334
Yamamoto A, Morioki H, Nakae T, Miyake Y, Harada T, Noda S, Mitsuoka S, Matsumoto K, Tomimatsu M, Kanemoto S, Tanaka S, Maeda M, Conway SJ, Imaizumi K, Fujio Y, Obana M.
Transcription factor old astrocyte specifically induced substance is a novel regulator of kidney fibrosis.
FASEB J
2021
PubMed ID: 33150680
DOI: 10.1096/fj.202001820R
|
|
20241
Miyake Y, Obana M, Nakae T, Yamamoto A, Tanaka S, Maeda M, Okada Y, Fujio Y.
PKNOX2 regulates myofibroblast functions and tubular cell survival during kidney fibrosis.
Biochem Biophys Res Commun
2021
571:88-95
PubMed ID: 34311199
DOI: 10.1016/j.bbrc.2021.07.067
|
|
6958
Yan Q, Ina K, Chiba S1, Wei H, Tatsukawa S, Fujikura Y.
The signal pathway for the repressive effect of dipyridamole on myofibroblast transdifferentiation.
J Cell Mol Med
2019
23(2):1608-1612
PubMed ID: 30451359
DOI: 10.1111/jcmm.14006
|
|
12256
Toshihiko Tsutsumi, Syougo Yamakawa, Akira Ishihara, Aimi Yamamoto, Tamotsu Tanaka, Akira Tokumura
Reduced kidney levels of lysophosphatidic acids in rats after chronic administration of aristolochic acid: Its possible protective role in renal fibrosis
Toxicol Rep
2015
2:121-129
PubMed ID: 28962344
DOI: 10.1016/j.toxrep.2015.02.012
|
|
15254
Yano R, Golbar HM, Izawa T, Sawamoto O, Kuwamura M, Yamate J.
Participation of bone morphogenetic protein (BMP)-6 and osteopontin in cisplatin (CDDP)-induced rat renal fibrosis
Exp Toxicol Pathol
2015
67(2):99-107
PubMed ID: 25467747
DOI: 10.1016/j.etp.2014.10.002
|
|
15703
Jeonghwa Kang, Makoto Sakuragi, Aya Shibata, Hiroshi Abe, Takashi Kitajima, Seiichi Tada, Masayoshi Mizutani, Hitoshi Ohmori, Hirohito Ayame, Tae Il Son, Toshiro Aigaki, Yoshihiro Ito
Immobilization of epidermal growth factor on titanium and stainless steel surfaces via dopamine treatment
Materials Science and Engineering: C
2012
32:2552-2561
DOI: 10.1016/j.msec.2012.07.039
|
|
16145
Nishi H, Inagi R, Kawada N, Yoshizato K, Mimura I, Fujita T, Nangaku M.
Cytoglobin, a novel member of the globin family, protects kidney fibroblasts against oxidative stress under ischemic conditions
Am J Pathol9
2011
178(1):128-3
PubMed ID: 21224051
DOI: 10.1016/j.ajpath.2010.11.011
|
|
8150
Mimura I, Nangaku M, Nishi H, Inagi R, Tanaka T, Fujita T.
Cytoglobin, a novel globin, plays an antifibrotic role in the kidney.
Am. J. Physiol. Renal Physiol.
2010
299:F1120-33
PubMed ID: 20719976
DOI: 10.1152/ajprenal.00145.2010
|
|
19133
Kitajima T, Sakuragi M, Hasuda H, Ozu T, Ito Y.
A chimeric epidermal growth factor with fibrin affinity promotes repair of injured keratinocyte sheets
Acta Biomater
2009
5(7):2623-32
PubMed ID: 19376761
DOI: 10.1016/j.actbio.2009.03.022
|
|
19088
Numazawa S, Sakaguchi H, Aoki R, Taira T, Yoshida T.
Regulation of the susceptibility to oxidative stress by cysteine availability in pancreatic beta-cells
Am J Physiol Cell Physiol
2008
295(2):C468-74
PubMed ID: 18524943
DOI: 10.1152/ajpcell.00203.2008
|
|
4734
Ishikawa E1, Tsuboi K, Takano S, Uchimura E, Nose T, Ohno T.
Intratumoral injection of IL-2-activated NK cells enhances the antitumor effect of intradermally injected paraformaldehyde-fixed tumor vaccine in a rat intracranial brain tumor model.
Cancer Sci
2004
95(1):98-103
PubMed ID: 14720334
DOI: 10.1111/j.1349-7006.2004.tb03177.x
|
|
1933
Han K, Hashimoto N, Ikeda Y, Shiratori Y, Kato H, Toda G, Komatsu Y, Yamada H, Tanaka A, Kurokawa K
Occurrence of antibody against rat hepatic sinusoidal endothelial cells in sera of patients with autoimmune hepatitis.
Dig Dis Sci
1995
40:1213-20
PubMed ID: 7781435
DOI: 10.1007/BF02065526
|
|
17924
Kurimoto M, Endo S, Arai K, Horie Y, Nogami K, Takaku A.
TM-1 cells from an established human malignant glioma cell line produce PDGF, TGF-alpha, and TGF-beta which cooperatively play a stimulatory role for an autocrine growth promotion
J Neurooncol
1994
22(1):33-44
PubMed ID: 7714549
DOI: 10.1007/BF01058353
|
|
1211
Yoshikatsu Ogawa, Yoshimitsu Yamazaki, Hiroaki Okuno
Stereoselectivity in the hydrolysis of synthetic esters by cultured cancer cells and normal tissue extracts of rat
Bioorganic & Medicinal Chemistry Letters
1994
4:757-760
DOI: 10.1016/s0960-894x(01)80196-7
|
戻る
細胞材料の再培養方法 (
HPS以外・
HPS )
ページトップへ
細胞材料開発室サイト