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Cell No. : Cell Name
RCB0112 : NRK49F  update : 2021/05/20
CommentContact-inhibited semi-normal cell line
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Ohno, Tadao
Originator Delarco, J. E.
Year of deposit 1986
Animal _rat < Mammals
Genus Rattus
Species norvegicus
Age at sampling adult
Tissue kidney
Classification semi-normal
Lifespan infinite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_2144
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives MEM + 5% CS
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Isozyme LD, NP
Chromosome mode 38-42(50) : 38(1),39(2),40(1),41(45),42(1)
Images
deposit info
lot info
Reference information Reference 4
User's Publication 10


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Reference
1815  Rizzino A  Behavior of transforming growth factors in serum-free media: an improved assay for transforming growth factors.  In Vitro  1984  20:815-22  PubMed ID: 6335125
1814  Bradshaw, G L, Dubes, G R  Supplementary factors required for serum-free culture of rat kidney cells of line NRK-49F.  In Vitro  1983  19:735-42  PubMed ID: 6354915
300  de Larco, J E, Todaro, G J  Epithelioid and fibroblastic rat kidney cell clones: epidermal growth factor (EGF) receptors and the effect of mouse sarcoma virus transformation.  J Cell Physiol  1978  94:335-42  PubMed ID: 304450
1806  Rosenblum, E N, Zeigel, R F, Zeigel RF  Persistent infection of a rat kidney cell line with Rauscher murine leukemia virus.  J Bacteriol  1966  92:1133-40  PubMed ID: 5926742

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User's Publication
13334  Yamamoto A, Morioki H, Nakae T, Miyake Y, Harada T, Noda S, Mitsuoka S, Matsumoto K, Tomimatsu M, Kanemoto S, Tanaka S, Maeda M, Conway SJ, Imaizumi K, Fujio Y, Obana M.  Transcription factor old astrocyte specifically induced substance is a novel regulator of kidney fibrosis.  FASEB J  2021    PubMed ID: 33150680
20241  Miyake Y, Obana M, Nakae T, Yamamoto A, Tanaka S, Maeda M, Okada Y, Fujio Y.  PKNOX2 regulates myofibroblast functions and tubular cell survival during kidney fibrosis.  Biochem Biophys Res Commun  2021  571:88-95  PubMed ID: 34311199
15439  Shams R, Matsukawa A, Ochi Y, Ito Y, Miyatake H.  In Silico and In Cell Hybrid Selection of Nonrapalog Ligands to Allosterically Inhibit the Kinase Activity of mTORC1  J Med Chem  2021  Jun 30  PubMed ID: 34191518
6958  Yan Q, Ina K, Chiba S1, Wei H, Tatsukawa S, Fujikura Y.  The signal pathway for the repressive effect of dipyridamole on myofibroblast transdifferentiation.  J Cell Mol Med  2019  23(2):1608-1612  PubMed ID: 30451359
12256  Toshihiko Tsutsumi, Syougo Yamakawa, Akira Ishihara, Aimi Yamamoto, Tamotsu Tanaka, Akira Tokumura  Reduced kidney levels of lysophosphatidic acids in rats after chronic administration of aristolochic acid: Its possible protective role in renal fibrosis  Toxicol Rep  2015  2:121-129  PubMed ID: 28962344
15254  Yano R, Golbar HM, Izawa T, Sawamoto O, Kuwamura M, Yamate J.  Participation of bone morphogenetic protein (BMP)-6 and osteopontin in cisplatin (CDDP)-induced rat renal fibrosis  Exp Toxicol Pathol  2015  67(2):99-107  PubMed ID: 25467747
15703  Jeonghwa Kang, Makoto Sakuragi, Aya Shibata, Hiroshi Abe, Takashi Kitajima, Seiichi Tada, Masayoshi Mizutani, Hitoshi Ohmori, Hirohito Ayame, Tae Il Son, Toshiro Aigaki, Yoshihiro Ito  Immobilization of epidermal growth factor on titanium and stainless steel surfaces via dopamine treatment  Materials Science and Engineering: C  2012  32:2552-2561 
8150  Mimura I, Nangaku M, Nishi H, Inagi R, Tanaka T, Fujita T.  Cytoglobin, a novel globin, plays an antifibrotic role in the kidney.  Am. J. Physiol. Renal Physiol.  2010  299:F1120-33  PubMed ID: 20719976
4734  Ishikawa E1, Tsuboi K, Takano S, Uchimura E, Nose T, Ohno T.  Intratumoral injection of IL-2-activated NK cells enhances the antitumor effect of intradermally injected paraformaldehyde-fixed tumor vaccine in a rat intracranial brain tumor model.  Cancer Sci  2004  95(1):98-103  PubMed ID: 14720334
1933  Han K, Hashimoto N, Ikeda Y, Shiratori Y, Kato H, Toda G, Komatsu Y, Yamada H, Tanaka A, Kurokawa K  Occurrence of antibody against rat hepatic sinusoidal endothelial cells in sera of patients with autoimmune hepatitis.  Dig Dis Sci  1995  40:1213-20  PubMed ID: 7781435



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