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Cell No. : Cell Name
RCB0112 : NRK49F  update : 2020/09/24
CommentContact-inhibited semi-normal cell line
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, a prior written permission of the DEPOSITOR or the RIKEN BRC is necessary.
Remarks
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
Basic information Depositor Ohno, Tadao
Originator Delarco, J. E.
Year of deposit 1986
Animal rat < Mammals
Genus Rattus
Species norvegicus
Age at sampling adult
Tissue kidney
Classification semi-normal
Lifespan infinite
Morphology fibroblast-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives MEM + 5% CS
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Isozyme LD, NP
Chromosome mode 38-42(50) : 38(1),39(2),40(1),41(45),42(1)
Images
deposit info
lot info
Reference information Reference 4
User's Publication 5


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Reference
1815  Rizzino A  Behavior of transforming growth factors in serum-free media: an improved assay for transforming growth factors.  In Vitro  1984  20:815-22  PubMed ID: 6335125
1814  Bradshaw, G L, Dubes, G R  Supplementary factors required for serum-free culture of rat kidney cells of line NRK-49F.  In Vitro  1983  19:735-42  PubMed ID: 6354915
300  de Larco, J E, Todaro, G J  Epithelioid and fibroblastic rat kidney cell clones: epidermal growth factor (EGF) receptors and the effect of mouse sarcoma virus transformation.  J Cell Physiol  1978  94:335-42  PubMed ID: 304450
1806  Rosenblum, E N, Zeigel, R F, Zeigel RF  Persistent infection of a rat kidney cell line with Rauscher murine leukemia virus.  J Bacteriol  1966  92:1133-40  PubMed ID: 5926742

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User's Publication
6958  Yan Q, Ina K, Chiba S1, Wei H, Tatsukawa S, Fujikura Y.  The signal pathway for the repressive effect of dipyridamole on myofibroblast transdifferentiation.  J Cell Mol Med  2019  23(2):1608-1612  PubMed ID: 30451359
12256  Toshihiko Tsutsumi, Syougo Yamakawa, Akira Ishihara, Aimi Yamamoto, Tamotsu Tanaka, Akira Tokumura  Reduced kidney levels of lysophosphatidic acids in rats after chronic administration of aristolochic acid: Its possible protective role in renal fibrosis  Toxicol Rep  2015  2:121-129  PubMed ID: 28962344
8150  Mimura I, Nangaku M, Nishi H, Inagi R, Tanaka T, Fujita T.  Cytoglobin, a novel globin, plays an antifibrotic role in the kidney.  Am. J. Physiol. Renal Physiol.  2010  299:F1120-33  PubMed ID: 20719976
4734  Ishikawa E1, Tsuboi K, Takano S, Uchimura E, Nose T, Ohno T.  Intratumoral injection of IL-2-activated NK cells enhances the antitumor effect of intradermally injected paraformaldehyde-fixed tumor vaccine in a rat intracranial brain tumor model.  Cancer Sci  2004  95(1):98-103  PubMed ID: 14720334
1933  Han K, Hashimoto N, Ikeda Y, Shiratori Y, Kato H, Toda G, Komatsu Y, Yamada H, Tanaka A, Kurokawa K  Occurrence of antibody against rat hepatic sinusoidal endothelial cells in sera of patients with autoimmune hepatitis.  Dig Dis Sci  1995  40:1213-20  PubMed ID: 7781435



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