Cell No. : Cell Name
RCB0102 : IC-2
update : 2024/11/15
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| Comment | Mast cell precursor, respond to IL-3. Conditioned medium. |
| Comment from the depositor | |
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
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Depositor |
Koyasu, Shigeo
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| Originator |
Koyasu, Shigeo
|
| Year of deposit |
1986
|
| Another name |
IC-2 820531
|
| Animal |
_mouse
< Mammals
|
| Gender |
Female
|
| Age at sampling |
4 weeks
|
| Tissue |
spleen
|
| Classification |
other
|
| Year of origin |
1981
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|
Lifespan |
infinite
|
| Morphology |
lymphocyte-like
|
| Cellosaurus(Expasy) |
CVCL_0341
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deposit info |
lot info |
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Medium |
Medium List |
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Culture type |
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Suspension cells
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| Culture method |
|
浮遊細胞の培養に関する一般的な注意(Japanese)
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| Culture medium |
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RPMI1640 + 10% FBS + 50% (RCB0035 WEHI-3 conditioned medium) + 50μM 2-mercaptoethanol
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| Antibiotics |
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Free
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|
Passage method |
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dilution
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Culture information
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Passage ratio |
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1 : 8 split
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| SC frequency |
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Subculture : 2 times/week
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| Temperature |
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37
℃
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| CO2 concentration |
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5
%
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| Freeze medium |
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Medium + 10% DMSO
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| Freezing method |
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Slow freezing
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| Mycoplasma/Acholeplasma |
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(-)
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| SSLP(mouse) |
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OK
|
| Isozyme |
|
LD, NP
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| Chromosome mode |
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31-39(50) : 31(1),36(2),37(1),38(5),39(41)
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| Images |
deposit info | lot info |
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| Reference information |
Reference |
2
|
| User's Publication |
8
|
| Reference |
|
1014
Koyasu S, Yodoi J, Nikaido T, Tagaya Y, Taniguchi Y, Honjo T, Yahara I
Expression of interleukin 2 receptors on interleukin 3-dependent cell lines.
J Immunol
1986
136:984-7
PubMed ID: 3079807
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1013
Koyasu S, Nakauchi H, Kitamura K, Yonehara S, Okumura K, Tada T, Yahara I
Production of interleukin 3 and gamma-interferon by an antigen-specific mouse suppressor T cell clone.
J Immunol
1985
134:3130-6
PubMed ID: 2580015
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| User's Publication |
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3446
Hu, Zhi-Qing, Zhao, Wei-Hua, Shimamura, Tadakatsu, Galli, Stephen J
Interleukin-4-triggered, STAT6-dependent production of a factor that induces mouse mast cell apoptosis.
Eur J Immunol
2006
36:1275-84
PubMed ID: 16598817
DOI: 10.1002/eji.200526275
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2898
Sasanuma H, Tatsuno A, Tsuji K, Hidano S, Morita S, Kitamura T, Kubo M, Kitamura D, Goitsuka R
Transcriptional regulation of SLP-76 family hematopoietic cell adaptor MIST/Clnk by STAT5.
Biochem Biophys Res Commun
2004
321:145-53
PubMed ID: 15358227
DOI: 10.1016/j.bbrc.2004.06.126
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1845
Hu, Z Q, Asano K, Yamazaki T, Shimamura T
Effect of lipopolysaccharide on mouse mast cell induction by a splenic cell culture system.
Infect Immun
1994
62:3844-9
PubMed ID: 7520422
DOI: 10.1128/IAI.62.9.3844-3849.1994
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1842
Konno S, Adachi M, Asano K, Gonogami Y, Ikeda K, Okamoto K, Takahashi T
Suppressive effects of IPD-1151T (suplatast-tosilate) on induction of mast cells from normal mouse splenocytes.
Eur J Pharmacol
1994
259:15-20
PubMed ID: 7957588
DOI: 10.1016/0014-2999(94)90151-1
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7040
Yu F, Itoyama Y, Kira J, Fujihara K, Kobayashi T, Kitamoto T, Suzumura A, Yamamoto N, Nakajima Y, Goto I.
TNF-beta produced by human T lymphotropic virus type I-infected cells influences the proliferation of human endothelial cells and fibroblasts.
J Immunol
1994
152(12):5930-8
PubMed ID: 8207218
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1733
Konno, S., Adachi, M., Asano, K., Okamoto, K. and Takahashi
Inhibitory effect of interferon - beta on mouse spleen - derived mast cells
Mediators of Inflammation
1993
2:243-246
PubMed ID: 18475529
DOI: 10.1155/S096293519300033X
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1743
Sawai T, Negishi M, Nishigaki N, Ohno T, Ichikawa A
Enhancement by protein kinase C of prostacyclin receptor-mediated activation of adenylate cyclase through a calmodulin/myristoylated alanine-rich C kinase substrate (MARCKS) system in IC2 mast cells.
J Biol Chem
1993
268:1995-2000
PubMed ID: 8380584
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1463
Asano K, Okamoto K
Murine T cell clones specific for Hymenolepis nana: generation and functional analysis in vivo and in vitro.
Int J Parasitol
1991
21:891-6
PubMed ID: 1787029
DOI: 10.1016/0020-7519(91)90163-2
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