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Cell No. : Cell Name
RCB0102 : IC-2  update : 2021/01/08
CommentMast cell precursor, respond to IL-3. Conditioned medium.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Koyasu, Shigeo
Originator Koyasu, Shigeo
Year of deposit 1986
Another name IC-2 820531
Animal _mouse < Mammals
Gender Female
Age at sampling 4 weeks
Tissue spleen
Classification other
Year of origin 1981
Lifespan infinite
Morphology lymphocyte-like
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives RPMI1640 + 10% FBS + 50% (RCB0035 WEHI-3 conditioned medium) + 50μM 2-mercaptoethanol
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
Chromosome mode 31-39(50) : 31(1),36(2),37(1),38(5),39(41)
Images
deposit info
lot info
Reference information Reference 2
User's Publication 8


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Reference
1014  Koyasu S, Yodoi J, Nikaido T, Tagaya Y, Taniguchi Y, Honjo T, Yahara I  Expression of interleukin 2 receptors on interleukin 3-dependent cell lines.  J Immunol  1986  136:984-7  PubMed ID: 3079807
1013  Koyasu S, Nakauchi H, Kitamura K, Yonehara S, Okumura K, Tada T, Yahara I  Production of interleukin 3 and gamma-interferon by an antigen-specific mouse suppressor T cell clone.  J Immunol  1985  134:3130-6  PubMed ID: 2580015

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User's Publication
3446  Hu, Zhi-Qing, Zhao, Wei-Hua, Shimamura, Tadakatsu, Galli, Stephen J  Interleukin-4-triggered, STAT6-dependent production of a factor that induces mouse mast cell apoptosis.  Eur J Immunol  2006  36:1275-84  PubMed ID: 16598817
2898  Sasanuma H, Tatsuno A, Tsuji K, Hidano S, Morita S, Kitamura T, Kubo M, Kitamura D, Goitsuka R  Transcriptional regulation of SLP-76 family hematopoietic cell adaptor MIST/Clnk by STAT5.  Biochem Biophys Res Commun  2004  321:145-53  PubMed ID: 15358227
1845  Hu, Z Q, Asano K, Yamazaki T, Shimamura T  Effect of lipopolysaccharide on mouse mast cell induction by a splenic cell culture system.  Infect Immun  1994  62:3844-9  PubMed ID: 7520422
1842  Konno S, Adachi M, Asano K, Gonogami Y, Ikeda K, Okamoto K, Takahashi T  Suppressive effects of IPD-1151T (suplatast-tosilate) on induction of mast cells from normal mouse splenocytes.  Eur J Pharmacol  1994  259:15-20  PubMed ID: 7957588
7040  Yu F, Itoyama Y, Kira J, Fujihara K, Kobayashi T, Kitamoto T, Suzumura A, Yamamoto N, Nakajima Y, Goto I.  TNF-beta produced by human T lymphotropic virus type I-infected cells influences the proliferation of human endothelial cells and fibroblasts.  J Immunol  1994  152(12):5930-8  PubMed ID: 8207218
1733  Konno, S., Adachi, M., Asano, K., Okamoto, K. and Takahashi  Inhibitory effect of interferon - beta on mouse spleen - derived mast cells  Mediators of Inflammation  1993  2:243-246  PubMed ID: 18475529
1743  Sawai T, Negishi M, Nishigaki N, Ohno T, Ichikawa A  Enhancement by protein kinase C of prostacyclin receptor-mediated activation of adenylate cyclase through a calmodulin/myristoylated alanine-rich C kinase substrate (MARCKS) system in IC2 mast cells.  J Biol Chem  1993  268:1995-2000  PubMed ID: 8380584
1463  Asano K, Okamoto K  Murine T cell clones specific for Hymenolepis nana: generation and functional analysis in vivo and in vitro.  Int J Parasitol  1991  21:891-6  PubMed ID: 1787029



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