Cell No. : Cell Name RCB0101 : L·P3  update : 2024/01/25
Comment			Protein- & lipid-free medium growing
Comment from the depositor
Terms and conditions			There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form			Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)
			Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
	Basic information	Depositor	Takaoka, Toshiko
		Originator	Katsuta,Hajim Takaoka,Toshiko
		Year of deposit	1986
		Animal	_mouse < Mammals
		Tissue	connective tissue
		Classification	other
		Lifespan	infinite
		Morphology	fibroblast-like
		Cellosaurus(Expasy)	CVCL_U990
			deposit info	lot info
		Medium	Medium List
		Culture type		Adherent cells
		Culture medium		DM-160AU
		Antibiotics		Free
		Passage method		scraping or dispase(1000U/ml)
	Culture information	Passage ratio		1 : 4 split
		SC frequency		Subculture : once/3-4 weeks, Medium Renewal : 2 times/week
		Temperature		37 ℃
		CO2 concentration		5 %
		Freeze medium		DM-160AU : 2 x FM1 = 1 : 1
		Freezing method		Slow freezing
		Mycoplasma/Acholeplasma		(-)
		SSLP(mouse)		OK
		Isozyme		LD, NP
Reference information		Reference	9
		User's Publication	2

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Reference

1012  Noda, K., Umeda, M. and Takaoka, T.  Growth of Cell Lines Which Can Grow in Chemically-defined Protein-free Medium Is not Necessarily Explained by the Autocrine Secretion of Transforming Growth Factors.  Yokohama Med. Bull.  1985  36:43226

1488  Ohno T, Okigaki T  IV-1 Growth Factors  Cell Struct Funct  1984  9:91-96

1002  Katsuta,H. and Takaoka,S.  Protein-and Lipid-Free Synthetic Media for The Cultivation of Mammalian Cells in Tissue Culture.  Nutritional Requirements of Cultured Cells.  1978  257-275

1010  Katsuta H, Takaoka T  Cultivation of cells in protein- and lipid-free synthetic media.  Methods Cell Biol  1973  6:1-42  PubMed ID: 4585079   DOI: 10.1016/s0091-679x(08)60046-1

1009  Takaoka T, Katsuta H  Long-term cultivation of mammalian cell strains in protein- and lipid-free chemically defined synthetic media.  Exp Cell Res  1971  67:295-304  PubMed ID: 4328993   DOI: 10.1016/0014-4827(71)90412-5

1011  Kagawa Y, Takaoka T, Katsuta H  Absence of Essential Fatty Acids in Mammalian Cell Strains Cultured in Lipid- and Protein-Free Chemically Definde Synthetic Media.  J Biochem (Tokyo)  1970  68:133-136  PubMed ID: 5452761

531  KATSUTA H, TAKAOKA T.  Amino acid requirements of a substrain of strain L cells (mouse fibroblasts) in protein-free chemically defined synthetic media.  Jpn J Exp Med  1960  30:235-59  PubMed ID: 14404774

1000  Katsuta,H., Takaoka,T., Mitamura,K., Kawada,I., Kuwabara,H., and Kuwabara,S.   Cultivation of Strain L Cells (Mouse Fibroblasts) in Proten-Free Media.  Jpn J Exp Med  1959  29:191-202  PubMed ID: 14404772

4657  Earle, W．R  Production of malignancy in vitro．Ⅳ．The mouse fibroblast cultures and changes seen in the living cells．  J. Nat. Cancer Inst．  1943  4:165-212

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User's Publication

2473  Tanaka I, Ishihara H  Enhanced expression of the early retrotransposon in C3H mouse-derived myeloid leukemia cells.  Virology  2001  280:107-14  PubMed ID: 11162824   DOI: 10.1006/viro.2000.0732

1567  Ohno, T., Kurita, K., Abe, S., Eimori, N., and Ikawa, Y.  A simple Freezing Medium for Serum-Free Cultured Cells  Cytotechnology  1988  1:257-260  PubMed ID: 22359122   DOI: 10.1007/BF00145029