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Cell No. : Cell Name
RCB0101 : L·P3  update : 2022/03/07
CommentProtein- & lipid-free medium growing
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Takaoka, Toshiko
Originator Katsuta,Hajim Takaoka,Toshiko
Year of deposit 1986
Animal mouse < Mammals
Tissue connective tissue
Classification other
Lifespan infinite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_U990
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DM-160AU
Antibiotics Free
Passage method scraping or dispase(1000U/ml)
Culture information Passage ratio 1 : 4 split
SC frequency Subculture : once/3-4 weeks, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium DM-160AU : 2 x FM1 = 1 : 1
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
Reference information Reference 9
User's Publication 2

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1012  Noda, K., Umeda, M. and Takaoka, T.  Growth of Cell Lines Which Can Grow in Chemically-defined Protein-free Medium Is not Necessarily Explained by the Autocrine Secretion of Transforming Growth Factors.  Yokohama Med. Bull.  1985  36:43226   
1488  Ohno T, Okigaki T  IV-1 Growth Factors  Cell Struct Funct  1984  9:91-96   
1002  Katsuta,H. and Takaoka,S.  Protein-and Lipid-Free Synthetic Media for The Cultivation of Mammalian Cells in Tissue Culture.  Nutritional Requirements of Cultured Cells.  1978  257-275   
1010  Katsuta H, Takaoka T  Cultivation of cells in protein- and lipid-free synthetic media.  Methods Cell Biol  1973  6:1-42  PubMed ID: 4585079   DOI: 10.1016/s0091-679x(08)60046-1
1009  Takaoka T, Katsuta H  Long-term cultivation of mammalian cell strains in protein- and lipid-free chemically defined synthetic media.  Exp Cell Res  1971  67:295-304  PubMed ID: 4328993   DOI: 10.1016/0014-4827(71)90412-5
1011  Kagawa Y, Takaoka T, Katsuta H  Absence of Essential Fatty Acids in Mammalian Cell Strains Cultured in Lipid- and Protein-Free Chemically Definde Synthetic Media.  J Biochem (Tokyo)  1970  68:133-136  PubMed ID: 5452761  
531  KATSUTA H, TAKAOKA T.  Amino acid requirements of a substrain of strain L cells (mouse fibroblasts) in protein-free chemically defined synthetic media.  Jpn J Exp Med  1960  30:235-59  PubMed ID: 14404774  
1000  Katsuta,H., Takaoka,T., Mitamura,K., Kawada,I., Kuwabara,H., and Kuwabara,S.   Cultivation of Strain L Cells (Mouse Fibroblasts) in Proten-Free Media.  Jpn J Exp Med  1959  29:191-202  PubMed ID: 14404772  
4657  Earle, W.R  Production of malignancy in vitro.Ⅳ.The mouse fibroblast cultures and changes seen in the living cells.  J. Nat. Cancer Inst.  1943  4:165-212   

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User's Publication
2473  Tanaka I, Ishihara H  Enhanced expression of the early retrotransposon in C3H mouse-derived myeloid leukemia cells.  Virology  2001  280:107-14  PubMed ID: 11162824   DOI: 10.1006/viro.2000.0732
1567  Ohno, T., Kurita, K., Abe, S., Eimori, N., and Ikawa, Y.  A simple Freezing Medium for Serum-Free Cultured Cells  Cytotechnology  1988  1:257-260  PubMed ID: 22359122  

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