Cell No. : Cell Name RCB0097 : CHL  update : 2024/11/13
Comment			Widely used in chromosome aberration test
Comment from the depositor
Terms and conditions			There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form			Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)
			Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
	Basic information	Depositor	Sutou, Shizuyo
		Originator	Koyama, H.
		Year of deposit	1986
		Animal	_hamster < Mammals
		Strain name	Chinese
		Genus	Cricetulus
		Species	griseus
		Gender	Female
		Age at sampling	3 days
		Tissue	lung
		Classification	semi-normal
		Lifespan	infinite
		Morphology	fibroblast-like
		Cellosaurus(Expasy)	CVCL_0212
			deposit info	lot info
		Medium	Medium List
		Culture type		Adherent cells
		Culture medium		MEM + 10% CS (heat inactivated)
		Antibiotics		Free
		Passage method		0.25% Trypsin
	Culture information	Passage ratio		1 : 8 split
		SC frequency		Subculture : 2 times/week
		Temperature		37 ℃
		CO2 concentration		5 %
		Freeze medium		Medium + 10% DMSO
		Freezing method		Slow freezing
		Mycoplasma/Acholeplasma		(-)
		Animal PCR		OK
		Isozyme		LD, NP
		Chromosome mode		23-26(50) : 23(6),24(5),25(34),26(5)
Images			deposit info	lot info
Reference information		Reference	4
		User's Publication	2

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Reference

70  Ishidate M Jr, Odashima S  Chromosome tests with 134 compounds on Chinese hamster cells in vitro--a screening for chemical carcinogens.  Mutat Res  1977  48:337-53  PubMed ID: 876270   DOI: 10.1016/0027-5107(77)90177-4

1006  Kato H, Yosida, T H  Isolation of aneusomic clones from Chinese hamster cell line following induction of nondisjunction.  Cytogenetics  1971  10:392-403  PubMed ID: 5169526   DOI: 10.1159/000130160

1008  Koyama H, Utakoji T, Ono T  A new cell line derived from newborn Chinese hamster lung tissue.  Gann  1970  61:161-7  PubMed ID: 5452405

1005  Hsu,T. C. and Zenzes,M. T.  Mammalian chromosomes in vitro. XVII. Idiogram of the Chinese hamster.  J Natl Cancer Inst  1964  32:857-869  PubMed ID: 19291904

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User's Publication

11467  Kishino Y, Hasegawa T, Arakawa S, Shibaya Y, Yamoto T, Mori K.  Effect of the metabolic capacity in rat liver S9 on the positive results of in vitro micronucleus tests.  J Toxicol Sci  2019    PubMed ID: 30842367   DOI: 10.2131/jts.44.145

18592  Kuga A, Kanagawa M, Sudo A, Chan YM, Tajiri M, Manya H, Kikkawa Y, Nomizu M, Kobayashi K, Endo T, Lu QL, Wada Y, Toda T.  Absence of post-phosphoryl modification in dystroglycanopathy mouse models and wild-type tissues expressing non-laminin binding form of α-dystroglycan  J Biol Chem  2012  287(12):9560-7  PubMed ID: 22270369   DOI: 10.1074/jbc.M111.271767