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Cell No. : Cell Name
RCB0097 : CHL  update : 2022/08/02
CommentWidely used in chromosome aberration test
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Sutou, Shizuyo
Originator Koyama, H.
Year of deposit 1986
Animal hamster < Mammals
Strain name Chinese
Genus Cricetulus
Species griseus
Gender Female
Age at sampling 3 days
Tissue lung
Classification semi-normal
Lifespan infinite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_0212
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives MEM + 10% CS (heat inactivated)
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Isozyme LD, NP
Chromosome mode 23-26(50) : 23(6),24(5),25(34),26(5)
Images
deposit info
lot info
Reference information Reference 4
User's Publication 1


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Reference
70  Ishidate M Jr, Odashima S  Chromosome tests with 134 compounds on Chinese hamster cells in vitro--a screening for chemical carcinogens.  Mutat Res  1977  48:337-53  PubMed ID: 876270
1006  Kato H, Yosida, T H  Isolation of aneusomic clones from Chinese hamster cell line following induction of nondisjunction.  Cytogenetics  1971  10:392-403  PubMed ID: 5169526
1008  Koyama H, Utakoji T, Ono T  A new cell line derived from newborn Chinese hamster lung tissue.  Gann  1970  61:161-7  PubMed ID: 5452405
1005  Hsu,T. C. and Zenzes,M. T.  Mammalian chromosomes in vitro. XVII. Idiogram of the Chinese hamster.  J Natl Cancer Inst  1964  32:857-869  PubMed ID: 19291904

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User's Publication
11467  Kishino Y, Hasegawa T, Arakawa S, Shibaya Y, Yamoto T, Mori K.  Effect of the metabolic capacity in rat liver S9 on the positive results of in vitro micronucleus tests.  J Toxicol Sci  2019    PubMed ID: 30842367



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