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Cell No. : Cell Name
RCB0096 : Don(D-6)  update : 2022/03/04
CommentPseudodiploid, two marker chromosomes
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Sutou, Shizuyo
Originator Kato H
Year of deposit 1986
Animal hamster < Mammals
Strain name Chinese
Genus Cricetulus
Species griseus
Gender Male
Age at sampling young
Tissue lung
Classification other
History National institute of Genetics-H.Kato
Lifespan infinite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_5634
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives MEM + 10% FBS + 1mM Sodium Pyruvate + 0.2mM L-Serine
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Isozyme LD, NP
Chromosome mode 19-26(50) : 19(1)20(4),21(19),22(16),23(8),24(1)/
Reference information Reference 3
User's Publication 0


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Reference
1007  Kato H, Yosida, T H  Banding patterns of Chinese hamster chromosomes revealed by new techniques.  Chromosoma  1972  36:272-80  PubMed ID: 4112624   DOI: 10.1007/bf00283246
1006  Kato H, Yosida, T H  Isolation of aneusomic clones from Chinese hamster cell line following induction of nondisjunction.  Cytogenetics  1971  10:392-403  PubMed ID: 5169526  
1005  Hsu,T. C. and Zenzes,M. T.  Mammalian chromosomes in vitro. XVII. Idiogram of the Chinese hamster.  J Natl Cancer Inst  1964  32:857-869  PubMed ID: 19291904  

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