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Cell No. : Cell Name
RCB0095 : P3/NSI/1-AG4-1  update : 2022/03/07
CommentMouse myeloma cell line. Utilized for genration of hybridoma. Reported in the literature to synthesize kappa chain, but do not secrete free light chain.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Ohno, Tadao
Originator Kohler, G. & Milstein, C.
Year of deposit 1986
Another name NS-1
Animal mouse < Mammals
Tissue myeloma
Disease name myeloma
Classification cancer
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_2155
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives RPMI1640 + 10% FBS + 1mM Sodium Pyruvate
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 10 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP, G6PD
Chromosome mode 60-65(50) : 60(2),61(1),62(16),63(23),64(5),65(3)
Images
deposit info
lot info
Reference information Reference 2
User's Publication 3


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Reference
4607  Köhler G, Milstein C.  Derivation of specific antibody-producing tissue culture and tumor lines by cell fusion.  Eur J Immunol  1976  6(7):511-9  PubMed ID: 825377   DOI: 10.1002/eji.1830060713
303  Eichmann K  Idiotype suppression. II. Amplification of a suppressor T cell with anti-idiotypic activity.  Eur J Immunol  1975  5:511-7  PubMed ID: 61874   DOI: 10.1002/eji.1830050802

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User's Publication
1926  Hitoshi Ohmori, Masayuki Raitoh, Mikiya Harano, Toshiyuki Oda, Masaki Hikida, Ryosuke Sugihara, Kazunaga Yazawa  Search for immunosuppressive substances produced in the culture of enteric bacteria isolated from marine fish  Journal of Fermentation and Bioengineering  1998  85:117-119    DOI: 10.1016/s0922-338x(97)80365-0
16968  Ohmori H, Shimada T, Hikida M, Takai T.  Phorbol ester-induced reversible inactivation of cytotoxic T cell function: correlation with down-regulation of protein kinase C activity  Jpn J Pharmacol  1994  66(4):427-32  PubMed ID: 7723218   DOI: 10.1254/jjp.66.427
15566  Ohmori H, Takai T, Tanigawa T, Honma Y.  Establishment of an enzyme release assay for cytotoxic T lymphocyte activity  J Immunol Methods  1992  147(1):119-24  PubMed ID: 1541835   DOI: 10.1016/s0022-1759(12)80036-6



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