Cell No. : Cell Name RCB0095 : P3/NSI/1-AG4-1  update : 2024/08/29
Comment			Mouse myeloma cell line. Utilized for genration of hybridoma. Reported in the literature to synthesize kappa chain, but do not secrete free light chain.
Comment from the depositor
Terms and conditions			There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form			Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)
			Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
	Basic information	Depositor	Ohno, Tadao
		Originator	Kohler, G. & Milstein, C.
		Year of deposit	1986
		Another name	NS-1
		Animal	_mouse < Mammals
		Tissue	myeloma
		Disease name	myeloma
		Classification	cancer
		Lifespan	infinite
		Morphology	lymphocyte-like
		Cellosaurus(Expasy)	CVCL_2155
			deposit info	lot info
		Medium	Medium List
		Culture type		Suspension cells
		Culture method		浮遊細胞の培養に関する一般的な注意(Japanese)
		Culture medium		RPMI1640 + 10% FBS + 1mM Sodium Pyruvate
		Antibiotics		Free
		Passage method		dilution
	Culture information	Passage ratio		1 : 10 split
		SC frequency		Subculture : 2 times/week
		Temperature		37 ℃
		CO2 concentration		5 %
		Freeze medium		Medium + 10% DMSO
		Freezing method		Slow freezing
		Mycoplasma/Acholeplasma		(-)
		SSLP(mouse)		OK
		Isozyme		LD, NP, G6PD
		Chromosome mode		60-65(50) : 60(2),61(1),62(16),63(23),64(5),65(3)
Images			deposit info	lot info
Reference information		Reference	2
		User's Publication	9

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Reference

4607  Köhler G, Milstein C.  Derivation of specific antibody-producing tissue culture and tumor lines by cell fusion.  Eur J Immunol  1976  6(7):511-9  PubMed ID: 825377   DOI: 10.1002/eji.1830060713

303  Eichmann K  Idiotype suppression. II. Amplification of a suppressor T cell with anti-idiotypic activity.  Eur J Immunol  1975  5:511-7  PubMed ID: 61874   DOI: 10.1002/eji.1830050802

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User's Publication

18616  Inoue T, Takashima M, Murakami S, Watanabe T.  Partial characterization of the antigen recognized by a monoclonal antibody to Ascaris suum ovary extracts  Vet Parasitol  2007  146(3-4):281-7  PubMed ID: 17391851   DOI: 10.1016/j.vetpar.2007.02.028

1207  Hitoshi Ohmori, Masayuki Raitoh, Mikiya Harano, Toshiyuki Oda, Masaki Hikida, Ryosuke Sugihara, Kazunaga Yazawa  Search for immunosuppressive substances produced in the culture of enteric bacteria isolated from marine fish  Journal of Fermentation and Bioengineering  1998  85:117-119    DOI: 10.1016/S0922-338X(97)80365-0

17944  Toshiyuki Takai, Tatsuji Yasuda, Hitoshi Ohmori  5-Azacytidine enhances transient expression of a transfected gene in cultured mammalian cells  Journal of Fermentation and Bioengineering  1995  79:617-619    DOI: 10.1016/0922-338x(95)94758-j

16968  Ohmori H, Shimada T, Hikida M, Takai T.  Phorbol ester-induced reversible inactivation of cytotoxic T cell function: correlation with down-regulation of protein kinase C activity  Jpn J Pharmacol  1994  66(4):427-32  PubMed ID: 7723218   DOI: 10.1254/jjp.66.427

17906  Takahiro Tanigawa, Masaki Hikida, Toshiyuki Takai, Tatsuji Yasuda, Hitoshi Ohmori  Enhancement of expression of an introduced gene by 5-azacytidine in mammalian cell lines  Journal of Fermentation and Bioengineering  1993  75:254-258    DOI: 10.1016/0922-338X(93)90147-Z

17909  Takahiro Tanigawa, Masaki Hikida, Toshiyuki Takai, Hitoshi Ohmori  Enhancement of expression of introduced β-galactosidase gene by 2-aminopurine in mammalian cell lines  Journal of Fermentation and Bioengineering  1993  76:145-147    DOI: 10.1016/0922-338X(93)90072-G

17893  Takai T, Ohmori H.  Enhancement of DNA transfection efficiency by heat treatment of cultured mammalian cells  Biochim Biophys Acta  1992  1129(2):161-5  PubMed ID: 1730054   DOI: 10.1016/0167-4781(92)90481-e

15566  Ohmori H, Takai T, Tanigawa T, Honma Y.  Establishment of an enzyme release assay for cytotoxic T lymphocyte activity  J Immunol Methods  1992  147(1):119-24  PubMed ID: 1541835   DOI: 10.1016/s0022-1759(12)80036-6

17878  Takai T, Ohmori H.  NA transfection of mouse lymphoid cells by the combination of DEAE-dextran-mediated DNA uptake and osmotic shock procedure  Biochim Biophys Acta  1990  1048(1):105-9  PubMed ID: 2297528   DOI: 10.1016/0167-4781(90)90029-2