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Cell No. : Cell Name
RCB0095 : P3/NSI/1-AG4-1  update : 2024/01/17
CommentMouse myeloma cell line. Utilized for genration of hybridoma. Reported in the literature to synthesize kappa chain, but do not secrete free light chain.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Ohno, Tadao
Originator Kohler, G. & Milstein, C.
Year of deposit 1986
Another name NS-1
Animal _mouse < Mammals
Tissue myeloma
Disease name myeloma
Classification cancer
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_2155
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Culture medium RPMI1640 + 10% FBS + 1mM Sodium Pyruvate
Culture method
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 10 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP, G6PD
Chromosome mode 60-65(50) : 60(2),61(1),62(16),63(23),64(5),65(3)
deposit info
lot info
Reference information Reference 2
User's Publication 9

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4607  Köhler G, Milstein C.  Derivation of specific antibody-producing tissue culture and tumor lines by cell fusion.  Eur J Immunol  1976  6(7):511-9  PubMed ID: 825377   DOI: 10.1002/eji.1830060713
303  Eichmann K  Idiotype suppression. II. Amplification of a suppressor T cell with anti-idiotypic activity.  Eur J Immunol  1975  5:511-7  PubMed ID: 61874   DOI: 10.1002/eji.1830050802

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User's Publication
18616  Inoue T, Takashima M, Murakami S, Watanabe T.  Partial characterization of the antigen recognized by a monoclonal antibody to Ascaris suum ovary extracts  Vet Parasitol  2007  146(3-4):281-7  PubMed ID: 17391851   DOI: 10.1016/j.vetpar.2007.02.028
1926  Hitoshi Ohmori, Masayuki Raitoh, Mikiya Harano, Toshiyuki Oda, Masaki Hikida, Ryosuke Sugihara, Kazunaga Yazawa  Search for immunosuppressive substances produced in the culture of enteric bacteria isolated from marine fish  Journal of Fermentation and Bioengineering  1998  85:117-119    DOI: 10.1016/s0922-338x(97)80365-0
17944  Toshiyuki Takai, Tatsuji Yasuda, Hitoshi Ohmori  5-Azacytidine enhances transient expression of a transfected gene in cultured mammalian cells  Journal of Fermentation and Bioengineering  1995  79:617-619    DOI: 10.1016/0922-338x(95)94758-j
16968  Ohmori H, Shimada T, Hikida M, Takai T.  Phorbol ester-induced reversible inactivation of cytotoxic T cell function: correlation with down-regulation of protein kinase C activity  Jpn J Pharmacol  1994  66(4):427-32  PubMed ID: 7723218   DOI: 10.1254/jjp.66.427
17906  Takahiro Tanigawa, Masaki Hikida, Toshiyuki Takai, Tatsuji Yasuda, Hitoshi Ohmori  Enhancement of expression of an introduced gene by 5-azacytidine in mammalian cell lines  Journal of Fermentation and Bioengineering  1993  75:254-258    DOI: 10.1016/0922-338X(93)90147-Z
17909  Takahiro Tanigawa, Masaki Hikida, Toshiyuki Takai, Hitoshi Ohmori  Enhancement of expression of introduced β-galactosidase gene by 2-aminopurine in mammalian cell lines  Journal of Fermentation and Bioengineering  1993  76:145-147    DOI: 10.1016/0922-338X(93)90072-G
17893  Takai T, Ohmori H.  Enhancement of DNA transfection efficiency by heat treatment of cultured mammalian cells  Biochim Biophys Acta  1992  1129(2):161-5  PubMed ID: 1730054   DOI: 10.1016/0167-4781(92)90481-e
15566  Ohmori H, Takai T, Tanigawa T, Honma Y.  Establishment of an enzyme release assay for cytotoxic T lymphocyte activity  J Immunol Methods  1992  147(1):119-24  PubMed ID: 1541835   DOI: 10.1016/s0022-1759(12)80036-6
17878  Takai T, Ohmori H.  NA transfection of mouse lymphoid cells by the combination of DEAE-dextran-mediated DNA uptake and osmotic shock procedure  Biochim Biophys Acta  1990  1048(1):105-9  PubMed ID: 2297528   DOI: 10.1016/0167-4781(90)90029-2

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