Cell No. : Cell Name
RCB0095 : P3/NSI/1-AG4-1
update : 2024/08/29
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Comment | Mouse myeloma cell line. Utilized for genration of hybridoma. Reported in the literature to synthesize kappa chain, but do not secrete free light chain. |
Comment from the depositor | |
Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
Remarks | |
Order Form |
Order Form(C-0005.pdf)
 
MTA(C-0007.pdf)
 
MTA(C-0007p.pdf)
 
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
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Depositor |
Ohno, Tadao
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Originator |
Kohler, G. & Milstein, C.
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Year of deposit |
1986
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Another name |
NS-1
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Animal |
_mouse
< Mammals
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Tissue |
myeloma
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Disease name |
myeloma
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Classification |
cancer
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Lifespan |
infinite
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Morphology |
lymphocyte-like
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Cellosaurus(Expasy) |
CVCL_2155
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deposit info |
lot info |
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Medium |
Medium List |
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Culture type |
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Suspension cells
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Culture method |
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浮遊細胞の培養に関する一般的な注意(Japanese)
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Culture medium |
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RPMI1640 + 10% FBS + 1mM Sodium Pyruvate
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Antibiotics |
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Free
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Passage method |
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dilution
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Culture information
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Passage ratio |
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1 : 10 split
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SC frequency |
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Subculture : 2 times/week
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Temperature |
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37
℃
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CO2 concentration |
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5
%
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Freeze medium |
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Medium + 10% DMSO
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Freezing method |
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Slow freezing
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Mycoplasma |
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(-)
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SSLP(mouse) |
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OK
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Isozyme |
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LD, NP, G6PD
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Chromosome mode |
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60-65(50) : 60(2),61(1),62(16),63(23),64(5),65(3)
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Images |
deposit info | lot info |
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Reference information |
Reference |
2
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User's Publication |
10
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User's Publication |
18616
Inoue T, Takashima M, Murakami S, Watanabe T.
Partial characterization of the antigen recognized by a monoclonal antibody to Ascaris suum ovary extracts
Vet Parasitol
2007
146(3-4):281-7
PubMed ID: 17391851
DOI: 10.1016/j.vetpar.2007.02.028
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1926
Hitoshi Ohmori, Masayuki Raitoh, Mikiya Harano, Toshiyuki Oda, Masaki Hikida, Ryosuke Sugihara, Kazunaga Yazawa
Search for immunosuppressive substances produced in the culture of enteric bacteria isolated from marine fish
Journal of Fermentation and Bioengineering
1998
85:117-119
DOI: 10.1016/s0922-338x(97)80365-0
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1207
Hitoshi Ohmori, Masayuki Raitoh, Mikiya Harano, Toshiyuki Oda, Masaki Hikida, Ryosuke Sugihara, Kazunaga Yazawa
Search for immunosuppressive substances produced in the culture of enteric bacteria isolated from marine fish
Journal of Fermentation and Bioengineering
1998
85:117-119
DOI: 10.1016/S0922-338X(97)80365-0
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17944
Toshiyuki Takai, Tatsuji Yasuda, Hitoshi Ohmori
5-Azacytidine enhances transient expression of a transfected gene in cultured mammalian cells
Journal of Fermentation and Bioengineering
1995
79:617-619
DOI: 10.1016/0922-338x(95)94758-j
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16968
Ohmori H, Shimada T, Hikida M, Takai T.
Phorbol ester-induced reversible inactivation of cytotoxic T cell function: correlation with down-regulation of protein kinase C activity
Jpn J Pharmacol
1994
66(4):427-32
PubMed ID: 7723218
DOI: 10.1254/jjp.66.427
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17906
Takahiro Tanigawa, Masaki Hikida, Toshiyuki Takai, Tatsuji Yasuda, Hitoshi Ohmori
Enhancement of expression of an introduced gene by 5-azacytidine in mammalian cell lines
Journal of Fermentation and Bioengineering
1993
75:254-258
DOI: 10.1016/0922-338X(93)90147-Z
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17909
Takahiro Tanigawa, Masaki Hikida, Toshiyuki Takai, Hitoshi Ohmori
Enhancement of expression of introduced β-galactosidase gene by 2-aminopurine in mammalian cell lines
Journal of Fermentation and Bioengineering
1993
76:145-147
DOI: 10.1016/0922-338X(93)90072-G
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17893
Takai T, Ohmori H.
Enhancement of DNA transfection efficiency by heat treatment of cultured mammalian cells
Biochim Biophys Acta
1992
1129(2):161-5
PubMed ID: 1730054
DOI: 10.1016/0167-4781(92)90481-e
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15566
Ohmori H, Takai T, Tanigawa T, Honma Y.
Establishment of an enzyme release assay for cytotoxic T lymphocyte activity
J Immunol Methods
1992
147(1):119-24
PubMed ID: 1541835
DOI: 10.1016/s0022-1759(12)80036-6
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17878
Takai T, Ohmori H.
NA transfection of mouse lymphoid cells by the combination of DEAE-dextran-mediated DNA uptake and osmotic shock procedure
Biochim Biophys Acta
1990
1048(1):105-9
PubMed ID: 2297528
DOI: 10.1016/0167-4781(90)90029-2
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