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細胞番号 : 細胞名
RCB0086 : FM3A  update : 2024/01/17
細胞特性(Comment:英)Mammary carcinoma, grow in vitro and in vivo
使用条件(英)There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
提供申込書類(英) Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
提供申込書類(日) 依頼書C-0001.pdf   同意書(非営利学術目的)C-0003.pdf   同意書(営利目的)C-0003p.pdf  
提供同意書は、使用機関の種類や目的に応じて、非営利学術目的 (C-XXXX) と営利目的 (C-XXXXp) の2種類があります。該当する提供同意書をご使用ください(詳細)。特許等の取得及び商業利用等は事前に必ず cellbank.brc@riken.jp までご連絡ください。
提供手数料 手数料とお支払いについてはこちらをご覧ください。
細胞基本情報 寄託者 Hanaoka, Fumio
樹立者 Saito, T. & Nakano, N.
寄託日 1986
別名 FM3A cl.28-5
動物種 _mouse < Mammals
動物系統名 C3H
性別 Female
採取組織 mammary
病名 mammary carcinoma
細胞分類 cancer
入手歴 Yokohama Uni.-H. Koyama
細胞寿命 infinite
細胞形態 lymphocyte-like
Cellosaurus(Expasy) CVCL_3869
培地・試薬情報 培地・試薬一覧はこちらをご覧ください。
培養形態 Suspension cells
培地 RPMI1640 + 10% CS
培養方法 浮遊細胞の培養に関する一般的な注意
抗生物質 Free
継代方法 dilution
継代密度 1 : 10 split
継代・培地交換頻度 Subculture : 2 times/week
培養最適温度 37 ℃
二酸化炭素濃度 5 %
凍結培地 Medium + 10% DMSO
凍結方法 Slow freezing
マイコプラズマ (-)
マウス系統検査 OK
アイソザイム検査 LD, NP
染色体検査 39-42(50) : 39(2),40(9),41(34),42(5)
文献情報 Reference(英) 8件
Reference(日) 8件
利用者成果(英) 17件
利用者成果(日) 0件

17475  Kazama H, Kashiwaba SI, Ishii S, Yoshida K, Yatsuo Y, Naraoka T, Fukuoka M, Murakami Y.  Loss of DIAPH3, a Formin Family Protein, Leads to Cytokinetic Failure Only under High Temperature Conditions in Mouse FM3A Cells  Int J Mol Sci  2020  21(22):8493  PubMed ID: 33187357   DOI: 10.3390/ijms21228493
1017  Murakami Y, Eki T, Yamada M, Prives C, Hurwitz J.  Species-specific in vitro synthesis of DNA containing the polyoma virus origin of replication  Proc Natl Acad Sci U S A  1986  83(17):6347-51  PubMed ID: 3018726   DOI: 10.1073/pnas.83.17.6347
149  Mineo C, Murakami Y, Ishimi Y, Hanaoka F, Yamada M.  Isolation and analysis of a mammalian temperature-sensitive mutant defective in G2 functions  Exp Cell Res  1986  167(1):53-62  PubMed ID: 2428648   DOI: 10.1016/0014-4827(86)90203-x
958  Yasumitsu H, Hanaoka F, Yasuda H, Murakami Y, Enomoto T, Yamada M  Isolation and initial characterization of a temperature-sensitive mutant of mouse FM3A cells defective in cytokinesis.  Cell Struct Funct  1985  10:79-88  PubMed ID: 2581712   DOI: 10.1247/csf.10.79
2596  Murakami Y, Yasuda H, Miyazawa H, Hanaoka F, Yamada M  Characterization of a temperature-sensitive mutant of mouse FM3A cells defective in DNA replication.  Proc Natl Acad Sci U S A  1985  82:1761-5  PubMed ID: 3856858   DOI: 10.1073/pnas.82.6.1761
941  Mita S, Yasuda H, Marunouchi T, Ishiko S, Yamada M.  A temperature-sensitive mutant of cultured mouse cells defective in chromosome condensation.  Exp Cell Res  1980  126(2):407-16  PubMed ID: 6153988   DOI: 10.1016/0014-4827(80)90280-3
118  Nakano N.  Establishment of cell lines in vitro from a mammary ascites tumor of mouse and biological properties of the established lines in a serum containing medium  Tohoku J Exp Med  1966  88(1):69-84  PubMed ID: 5920633   DOI: 10.1620/tjem.88.69
119  Yamane I, Nakano N.  Long-term culture of the ascites form of a mouse mammary tumor in serum-free media  Tohoku J Exp Med  1966  88(3):203-14  PubMed ID: 5956504  

1705  小山秀機  マウス乳癌 : FM3A  生体の科学  1992  43:365   
1277  榎本武美  バイオテクノロジ-素材としての培養細胞 生化学実験  蛋白質 核酸 酵素  1990  35:1577-1578   
1225  小山秀機  バイオテクノロジ-素材としての培養細胞 体細胞遺伝学  蛋白質 核酸 酵素  1988  33:288-289   
1244  榎本武美  バイオテクノロジ-素材としての培養細胞 細胞周期  蛋白質 核酸 酵素  1988  33:1326-1327   
1254  森田初恵  バイオテクノロジ-素材としての培養細胞 突然変異・細胞毒性アッセイ  蛋白質 核酸 酵素  1988  33:1828   
1256  鮎沢大  バイオテクノロジ-素材としての培養細胞 遺伝子移入と発現  蛋白質 核酸 酵素  1988  33:2404   
90  koyama, Hideki  FM3A株  組織培養  1980  6:235-243   
91  Saito, Takero  C3H マウス乳癌の腹水型転換---マウス腹水乳癌FM3Aの樹立  福島医学雑誌  1962  12:335-352   

20630  Fujii T, Kamishikiryo J, Morita T.  Palbociclib regulates intracellular lipids in mammary tumor cells by secreting lipoprotein lipase.  Pharmacol Rep  2022    PubMed ID: 35366749   DOI: 10.1007/s43440-022-00365-0
19499  Nguyen TD, Miyatake Y, Yoshida T, Kawahara H, Hanayama R.  Tumor-secreted proliferin-1 regulates adipogenesis and lipolysis in cachexia  Int J Cancer  2021  148(8):1982-1992  PubMed ID: 33252827   DOI: 10.1002/ijc.33418
20430  Makino A, Okazawa H, Kiyono Y.  Utilization of Antibody Allows Rapid Clearance of Nanoparticle Probes from Blood without the Need of Probe Modifications.  ACS Omega  2021  6(32):21153-21159  PubMed ID: 34423223   DOI: 10.1021/acsomega.1c03076
13195  Fujii T, Ogasawara M, Kamishikiryo J, Morita T.  β-Estradiol Enhanced Secretion of Lipoprotein Lipase from Mouse Mammary Tumor FM3A Cells.  Biol Pharm Bull  2020    PubMed ID: 32879215   DOI: 10.1248/bpb.b20-00408
4186  Iwai T, Sugimoto M, Wakita D, Yorozu K, Kurasawa M, Yamamoto K.  Topoisomerase I inhibitor, irinotecan, depletes regulatory T cells and up-regulates MHC class I and PD-L1 expression, resulting in a supra-additive antitumor effect when combined with anti-PD-L1 antibodies.  Oncotarget  2018    PubMed ID: 30140379   DOI: 10.18632/oncotarget.25830
8226  Matsunaga A, Ueda Y, Yamada S, Harada Y, Shimada H, Hasegawa M, Tsujii H, Ochiai T, Yonemitsu Y.  Carbon-ion beam treatment induces systemic antitumor immunity against murine squamous cell carcinoma.  Cancer  2010  116:3740-8  PubMed ID: 20564091   DOI: 10.1002/cncr.25134
8349  Harada M, Kubo H, Abe T, Maezawa H, Otsuka H.  Selection of endogenous 13C substrates for observation of intracellular metabolism using the dynamic nuclear polarization technique.  Jpn J Radiol  2010  28:173-9  PubMed ID: 20182855   DOI: 10.1007/s11604-009-0390-8
8658  Castle JC, Hara Y, Raymond CK, Garrett-Engele P, Ohwaki K, Kan Z, Kusunoki J, Johnson JM.  ACC2 is expressed at high levels in human white adipose and has an isoform with a novel N-terminus [corrected].  PLoS ONE  2009  4:e4369  PubMed ID: 19190759   DOI: 10.1371/journal.pone.0004369
13720  JB Lee et al  Anticancer efficacies of doxorubicin, verapamil and quercetin on FM3A cells under hyperthermic temperature  Biotechnology and Bioprocess Engineering  2004  9:261-266   
17306  Jeong Beom Lee, Jun Sang Bae, Jeong Hwan Choi, Joo Hyen Ham, Young Ki Min, Hun Mo Yang, Timothy Othman, Kazuhiro Shimizu  Anticancer efficacies of doxorubicin, verapamil and quercetin on FM3A cells under hyperthermic temperature  Biotechnology and Bioprocess Engineering  2004  9:261-266    DOI: 10.1007/BF02942341
13676  Othman T, Goto S, Lee JB, Taimura A, Matsumoto T, Kosaka M.  Hyperthermic enhancement of the apoptotic and antiproliferative activities of paclitaxel  Pharmacology  2001  62(4):208-12  PubMed ID: 11359996   DOI: 10.1159/000056096
15540  Yokota S, Yanagi H, Yura T, Kubota H.  Cytosolic chaperonin-containing t-complex polypeptide 1 changes the content of a particular subunit species concomitant with substrate binding and folding activities during the cell cycle  Eur J Biochem  2001  268(17):4664-73. d  PubMed ID: 11532003   DOI: 10.1046/j.1432-1327.2001.02393.x
2951  Yokota S, Kayano T, Ohta T, Kurimoto M, Yanagi H, Yura T, Kubota H  Proteasome-dependent degradation of cytosolic chaperonin CCT  Biochemical and biophysical research communications  2000  279:712-717  PubMed ID: 11118350   DOI: 10.1006/bbrc.2000.4011
15669  TIMOTHY OTHMAN, SHINJI GOTO, JEONG-BEOM LEE, TAKAAKI MATSUMOTO, AKIHIRO TAIMURA, EIKO KANEDA, NOBU OHWATARI, MITSUO KOSAKA  Antiproliferative Efficacies of Doxorubicin, Verapamil, Paclitaxel and Quercetin in FM3A Cells at Hyperthermic Temperature. A Comparative Assessment.  Thermal Medicine(Japanese Journal of Hyperthermic Oncology)  2000  16:37-47    DOI: 10.3191/thermalmedicine.16.37
2380  Yokota S, Yanagi H, Yura T, Kubota H  Cytosolic chaperonin is up-regulated during cell growth. Preferential expression and binding to tubulin at G(1)/S transition through early S phase.  J Biol Chem  1999  274:37070-8  PubMed ID: 10601265   DOI: 10.1074/jbc.274.52.37070
1928  Osamu Murata, Hideyuki Sakurai, Norio Mitsuhashi, Hideo Niibe  31P magnetic resonance spectroscopy for estimating the radiation response of a murine mammary tumor  Annals of Cancer Research and Therapy  1998  6:109-112    DOI: 10.4993/acrt1992.6.109
1399  Eki T, Enomoto T, Masutani C, Miyajima A, Takada R, Murakami Y, Ohno T, Hanaoka F, Ui M  Mouse DNA primase plays the principal role in determination of permissiveness for polyomavirus DNA replication.  J Virol  1991  65:4874-81  PubMed ID: 1651410   DOI: 10.1128/JVI.65.9.4874-4881.1991


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