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Cell No. : Cell Name
RCB0086 : FM3A  update : 2024/01/17
CommentMammary carcinoma, grow in vitro and in vivo
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Hanaoka, Fumio
Originator Saito, T. & Nakano, N.
Year of deposit 1986
Another name FM3A cl.28-5
Animal _mouse < Mammals
Strain name C3H
Gender Female
Tissue mammary
Disease name mammary carcinoma
Classification cancer
History Yokohama Uni.-H. Koyama
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_3869
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Culture medium RPMI1640 + 10% CS
Culture method
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 10 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
Chromosome mode 39-42(50) : 39(2),40(9),41(34),42(5)
deposit info
lot info
Reference information Reference 8
User's Publication 17

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17475  Kazama H, Kashiwaba SI, Ishii S, Yoshida K, Yatsuo Y, Naraoka T, Fukuoka M, Murakami Y.  Loss of DIAPH3, a Formin Family Protein, Leads to Cytokinetic Failure Only under High Temperature Conditions in Mouse FM3A Cells  Int J Mol Sci  2020  21(22):8493  PubMed ID: 33187357   DOI: 10.3390/ijms21228493
1017  Murakami Y, Eki T, Yamada M, Prives C, Hurwitz J.  Species-specific in vitro synthesis of DNA containing the polyoma virus origin of replication  Proc Natl Acad Sci U S A  1986  83(17):6347-51  PubMed ID: 3018726   DOI: 10.1073/pnas.83.17.6347
149  Mineo C, Murakami Y, Ishimi Y, Hanaoka F, Yamada M.  Isolation and analysis of a mammalian temperature-sensitive mutant defective in G2 functions  Exp Cell Res  1986  167(1):53-62  PubMed ID: 2428648   DOI: 10.1016/0014-4827(86)90203-x
958  Yasumitsu H, Hanaoka F, Yasuda H, Murakami Y, Enomoto T, Yamada M  Isolation and initial characterization of a temperature-sensitive mutant of mouse FM3A cells defective in cytokinesis.  Cell Struct Funct  1985  10:79-88  PubMed ID: 2581712   DOI: 10.1247/csf.10.79
2596  Murakami Y, Yasuda H, Miyazawa H, Hanaoka F, Yamada M  Characterization of a temperature-sensitive mutant of mouse FM3A cells defective in DNA replication.  Proc Natl Acad Sci U S A  1985  82:1761-5  PubMed ID: 3856858   DOI: 10.1073/pnas.82.6.1761
941  Mita S, Yasuda H, Marunouchi T, Ishiko S, Yamada M.  A temperature-sensitive mutant of cultured mouse cells defective in chromosome condensation.  Exp Cell Res  1980  126(2):407-16  PubMed ID: 6153988   DOI: 10.1016/0014-4827(80)90280-3
118  Nakano N.  Establishment of cell lines in vitro from a mammary ascites tumor of mouse and biological properties of the established lines in a serum containing medium  Tohoku J Exp Med  1966  88(1):69-84  PubMed ID: 5920633   DOI: 10.1620/tjem.88.69
119  Yamane I, Nakano N.  Long-term culture of the ascites form of a mouse mammary tumor in serum-free media  Tohoku J Exp Med  1966  88(3):203-14  PubMed ID: 5956504  

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User's Publication
20630  Fujii T, Kamishikiryo J, Morita T.  Palbociclib regulates intracellular lipids in mammary tumor cells by secreting lipoprotein lipase.  Pharmacol Rep  2022    PubMed ID: 35366749   DOI: 10.1007/s43440-022-00365-0
19499  Nguyen TD, Miyatake Y, Yoshida T, Kawahara H, Hanayama R.  Tumor-secreted proliferin-1 regulates adipogenesis and lipolysis in cachexia  Int J Cancer  2021  148(8):1982-1992  PubMed ID: 33252827   DOI: 10.1002/ijc.33418
20430  Makino A, Okazawa H, Kiyono Y.  Utilization of Antibody Allows Rapid Clearance of Nanoparticle Probes from Blood without the Need of Probe Modifications.  ACS Omega  2021  6(32):21153-21159  PubMed ID: 34423223   DOI: 10.1021/acsomega.1c03076
13195  Fujii T, Ogasawara M, Kamishikiryo J, Morita T.  β-Estradiol Enhanced Secretion of Lipoprotein Lipase from Mouse Mammary Tumor FM3A Cells.  Biol Pharm Bull  2020    PubMed ID: 32879215   DOI: 10.1248/bpb.b20-00408
4186  Iwai T, Sugimoto M, Wakita D, Yorozu K, Kurasawa M, Yamamoto K.  Topoisomerase I inhibitor, irinotecan, depletes regulatory T cells and up-regulates MHC class I and PD-L1 expression, resulting in a supra-additive antitumor effect when combined with anti-PD-L1 antibodies.  Oncotarget  2018    PubMed ID: 30140379   DOI: 10.18632/oncotarget.25830
8226  Matsunaga A, Ueda Y, Yamada S, Harada Y, Shimada H, Hasegawa M, Tsujii H, Ochiai T, Yonemitsu Y.  Carbon-ion beam treatment induces systemic antitumor immunity against murine squamous cell carcinoma.  Cancer  2010  116:3740-8  PubMed ID: 20564091   DOI: 10.1002/cncr.25134
8349  Harada M, Kubo H, Abe T, Maezawa H, Otsuka H.  Selection of endogenous 13C substrates for observation of intracellular metabolism using the dynamic nuclear polarization technique.  Jpn J Radiol  2010  28:173-9  PubMed ID: 20182855   DOI: 10.1007/s11604-009-0390-8
8658  Castle JC, Hara Y, Raymond CK, Garrett-Engele P, Ohwaki K, Kan Z, Kusunoki J, Johnson JM.  ACC2 is expressed at high levels in human white adipose and has an isoform with a novel N-terminus [corrected].  PLoS ONE  2009  4:e4369  PubMed ID: 19190759   DOI: 10.1371/journal.pone.0004369
13720  JB Lee et al  Anticancer efficacies of doxorubicin, verapamil and quercetin on FM3A cells under hyperthermic temperature  Biotechnology and Bioprocess Engineering  2004  9:261-266   
17306  Jeong Beom Lee, Jun Sang Bae, Jeong Hwan Choi, Joo Hyen Ham, Young Ki Min, Hun Mo Yang, Timothy Othman, Kazuhiro Shimizu  Anticancer efficacies of doxorubicin, verapamil and quercetin on FM3A cells under hyperthermic temperature  Biotechnology and Bioprocess Engineering  2004  9:261-266    DOI: 10.1007/BF02942341
13676  Othman T, Goto S, Lee JB, Taimura A, Matsumoto T, Kosaka M.  Hyperthermic enhancement of the apoptotic and antiproliferative activities of paclitaxel  Pharmacology  2001  62(4):208-12  PubMed ID: 11359996   DOI: 10.1159/000056096
15540  Yokota S, Yanagi H, Yura T, Kubota H.  Cytosolic chaperonin-containing t-complex polypeptide 1 changes the content of a particular subunit species concomitant with substrate binding and folding activities during the cell cycle  Eur J Biochem  2001  268(17):4664-73. d  PubMed ID: 11532003   DOI: 10.1046/j.1432-1327.2001.02393.x
2951  Yokota S, Kayano T, Ohta T, Kurimoto M, Yanagi H, Yura T, Kubota H  Proteasome-dependent degradation of cytosolic chaperonin CCT  Biochemical and biophysical research communications  2000  279:712-717  PubMed ID: 11118350   DOI: 10.1006/bbrc.2000.4011
15669  TIMOTHY OTHMAN, SHINJI GOTO, JEONG-BEOM LEE, TAKAAKI MATSUMOTO, AKIHIRO TAIMURA, EIKO KANEDA, NOBU OHWATARI, MITSUO KOSAKA  Antiproliferative Efficacies of Doxorubicin, Verapamil, Paclitaxel and Quercetin in FM3A Cells at Hyperthermic Temperature. A Comparative Assessment.  Thermal Medicine(Japanese Journal of Hyperthermic Oncology)  2000  16:37-47    DOI: 10.3191/thermalmedicine.16.37
2380  Yokota S, Yanagi H, Yura T, Kubota H  Cytosolic chaperonin is up-regulated during cell growth. Preferential expression and binding to tubulin at G(1)/S transition through early S phase.  J Biol Chem  1999  274:37070-8  PubMed ID: 10601265   DOI: 10.1074/jbc.274.52.37070
1928  Osamu Murata, Hideyuki Sakurai, Norio Mitsuhashi, Hideo Niibe  31P magnetic resonance spectroscopy for estimating the radiation response of a murine mammary tumor  Annals of Cancer Research and Therapy  1998  6:109-112    DOI: 10.4993/acrt1992.6.109
1399  Eki T, Enomoto T, Masutani C, Miyajima A, Takada R, Murakami Y, Ohno T, Hanaoka F, Ui M  Mouse DNA primase plays the principal role in determination of permissiveness for polyomavirus DNA replication.  J Virol  1991  65:4874-81  PubMed ID: 1651410   DOI: 10.1128/JVI.65.9.4874-4881.1991

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