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Cell No. : Cell Name
RCB0086 : FM3A  update : 2021/02/26
CommentMammary carcinoma, grow in vitro and in vivo
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Hanaoka, Fumio
Originator Saito, T. & Nakano, N.
Year of deposit 1986
Another name FM3A cl.28-5
Animal _mouse < Mammals
Strain name C3H
Gender Female
Tissue mammary
Disease name mammary carcinoma
Classification cancer
History Yokohama Uni.-H. Koyama
Lifespan infinite
Morphology lymphocyte-like
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives RPMI1640 + 10% CS
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 10 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
Chromosome mode 39-42(50) : 39(2),40(9),41(34),42(5)
deposit info
lot info
Reference information Reference 3
User's Publication 10

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2596  Murakami Y, Yasuda H, Miyazawa H, Hanaoka F, Yamada M  Characterization of a temperature-sensitive mutant of mouse FM3A cells defective in DNA replication.  Proc Natl Acad Sci U S A  1985  82:1761-5  PubMed ID: 3856858
941  Mita S, Yasuda H, Marunouchi T, Ishiko S, Yamada M.  A temperature-sensitive mutant of cultured mouse cells defective in chromosome condensation.  Exp Cell Res  1980  126(2):407-16  PubMed ID: 6153988
92  Yamane I, Nakano N  Long-term culture of the ascites form of a mouse mammary tumor in serum-free media.  Tohoku J Exp Med  1966  88:203-14  PubMed ID: 5956504

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User's Publication
13195  Fujii T, Ogasawara M, Kamishikiryo J, Morita T.  β-Estradiol Enhanced Secretion of Lipoprotein Lipase from Mouse Mammary Tumor FM3A Cells.  Biol Pharm Bull  2020    PubMed ID: 32879215
11603  Aoki N, Matsui Y.  Comprehensive Analysis of Mouse Cancer/Testis Antigen Functions in Cancer Cells and Roles of TEKT5 in Cancer Cells and Testicular Germ Cells.  Mol. Cell. Biol.  2019    PubMed ID: 31208979
4186  Iwai T, Sugimoto M, Wakita D, Yorozu K, Kurasawa M, Yamamoto K.  Topoisomerase I inhibitor, irinotecan, depletes regulatory T cells and up-regulates MHC class I and PD-L1 expression, resulting in a supra-additive antitumor effect when combined with anti-PD-L1 antibodies.  Oncotarget  2018    PubMed ID: 30140379
8226  Matsunaga A, Ueda Y, Yamada S, Harada Y, Shimada H, Hasegawa M, Tsujii H, Ochiai T, Yonemitsu Y.  Carbon-ion beam treatment induces systemic antitumor immunity against murine squamous cell carcinoma.  Cancer  2010  116:3740-8  PubMed ID: 20564091
8349  Harada M, Kubo H, Abe T, Maezawa H, Otsuka H.  Selection of endogenous 13C substrates for observation of intracellular metabolism using the dynamic nuclear polarization technique.  Jpn J Radiol  2010  28:173-9  PubMed ID: 20182855
8658  Castle JC, Hara Y, Raymond CK, Garrett-Engele P, Ohwaki K, Kan Z, Kusunoki J, Johnson JM.  ACC2 is expressed at high levels in human white adipose and has an isoform with a novel N-terminus [corrected].  PLoS ONE  2009  4:e4369  PubMed ID: 19190759
13720  JB Lee et al  Anticancer efficacies of doxorubicin, verapamil and quercetin on FM3A cells under hyperthermic temperature  Biotechnology and Bioprocess Engineering  2004  9:261-266 
13676  Othman T, Goto S, Lee JB, Taimura A, Matsumoto T, Kosaka M.  Hyperthermic enhancement of the apoptotic and antiproliferative activities of paclitaxel  Pharmacology  2001  62(4):208-12  PubMed ID: 11359996
2380  Yokota S, Yanagi H, Yura T, Kubota H  Cytosolic chaperonin is up-regulated during cell growth. Preferential expression and binding to tubulin at G(1)/S transition through early S phase.  J Biol Chem  1999  274:37070-8  PubMed ID: 10601265
1399  Eki T, Enomoto T, Masutani C, Miyajima A, Takada R, Murakami Y, Ohno T, Hanaoka F, Ui M  Mouse DNA primase plays the principal role in determination of permissiveness for polyomavirus DNA replication.  J Virol  1991  65:4874-81  PubMed ID: 1651410

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