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Cell No. : Cell Name
RCB0076 : LYM-1  update : 2022/03/04
CommentSticky cell from lymph node.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Takaoka, Toshiko
Originator Kurayama, Hideo
Year of deposit 1986
Animal rat < Mammals
Tissue lymph node
Classification other
Year of origin 1982
Lifespan infinite
Morphology other
Cellosaurus(Expasy) CVCL_4859
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DM-160AU + 2% FBS (add 10% FBS for initial cell attachment after thawing)
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Isozyme LD, NP, G6PD
Reference information Reference 2
User's Publication 2


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Reference
5459  Wang XM, Kojima T, Satoh K, Taniguchi Y, Tokumaru K, Saifuku K, Seki M, Kihira K, Ido K, Uchida JY, Ohmori C, Takaoka T, Kimura K.  The value of LYM-1 cells for examining vacuole formation and loss of cell viability induced by culture supernates of Helicobacter pylori.  J Med Microbiol  1997  46(8):705-9  PubMed ID: 9511820
1088  Kurayama H, Ogata S, Nanba M, Ideguchi S, Takaoka T, Shida S.  Establishment of a new cell line secreting colony stimulating factor and growth inhibiting factor from metastatic lymph nodes of a rat.   Dokhyo JMed Sci  1986  13: 9-13 

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User's Publication
2365  Takase-Yoden S, Watanabe R  Contribution of virus-receptor interaction to distinct viral proliferation of neuropathogenic and nonneuropathogenic murine leukemia viruses in rat glial cells.  J Virol  1999  73:4461-4  PubMed ID: 10196347
6246  Ohsawa K, Watanabe Y, Sato H.  Hantaan virus persistently infects marmoset B-lymphoblastoid cell line.  J Vet Med Sci  1995  57(5):931-4  PubMed ID: 8593305



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