Cell No. : Cell Name RCB0076 : LYM-1  update : 2023/01/04
Comment			Sticky cell from lymph node.
Comment from the depositor
Terms and conditions			There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form			Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)
			Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
	Basic information	Depositor	Takaoka, Toshiko
		Originator	Kurayama, Hideo
		Year of deposit	1986
		Animal	_rat < Mammals
		Tissue	lymph node
		Classification	other
		Year of origin	1982
		Lifespan	infinite
		Morphology	other
		Cellosaurus(Expasy)	CVCL_4859
			deposit info	lot info
		Medium	Medium List
		Culture type		Adherent cells
		Culture medium		DM-160AU + 2% FBS (add 10% FBS for initial cell attachment after thawing)
		Antibiotics		Free
		Passage method		0.25% Trypsin
	Culture information	Passage ratio		1 : 8 split
		SC frequency		Subculture : 2 times/week
		Temperature		37 ℃
		CO2 concentration		5 %
		Freeze medium		Medium + 10% DMSO
		Freezing method		Slow freezing
		Mycoplasma/Acholeplasma		(-)
		Animal PCR		OK
		Isozyme		LD, NP, G6PD
Reference information		Reference	2
		User's Publication	2

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Reference

5459  Wang XM, Kojima T, Satoh K, Taniguchi Y, Tokumaru K, Saifuku K, Seki M, Kihira K, Ido K, Uchida JY, Ohmori C, Takaoka T, Kimura K.  The value of LYM-1 cells for examining vacuole formation and loss of cell viability induced by culture supernates of Helicobacter pylori.  J Med Microbiol  1997  46(8):705-9  PubMed ID: 9511820   DOI: 10.1099/00222615-46-8-705

1088  Kurayama H, Ogata S, Nanba M, Ideguchi S, Takaoka T, Shida S.  Establishment of a new cell line secreting colony stimulating factor and growth inhibiting factor from metastatic lymph nodes of a rat.   Dokhyo JMed Sci  1986  13: 9-13

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User's Publication

2365  Takase-Yoden S, Watanabe R  Contribution of virus-receptor interaction to distinct viral proliferation of neuropathogenic and nonneuropathogenic murine leukemia viruses in rat glial cells.  J Virol  1999  73:4461-4  PubMed ID: 10196347   DOI: 10.1128/JVI.73.5.4461-4464.1999

6246  Ohsawa K, Watanabe Y, Sato H.  Hantaan virus persistently infects marmoset B-lymphoblastoid cell line.  J Vet Med Sci  1995  57(5):931-4  PubMed ID: 8593305   DOI: 10.1292/jvms.57.931