Cell No. : Cell Name RCB0075 : RCR-1  update : 2021/01/08
Comment			Astroglia cell line
Comment from the depositor
Terms and conditions			There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form			Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
	Basic information	Depositor	Takaoka, Toshiko
		Originator	Jou,Teh-Cheng et al.
		Year of deposit	1986
		Animal	rat < Mammals
		Age at sampling	3 days
		Tissue	brain
		Classification	other
		Year of origin	1979
		Memo_1	Mycoplasma was eliminated with MC-210 by the depositor.
		Lifespan	infinite
		Morphology	other
			deposit info	lot info
		Medium	Medium List
		Culture type		Adherent cells
		Medium and additives		DM-160AU + 10% FBS
		Antibiotics		Free
		Passage method		0.25% trypsin
	Culture information	Passage ratio		1 : 8 split
		SC frequency		Subculture : 2 times/week
		Temperature		37 ℃
		CO2 concentration		5 %
		Freeze medium		Medium + 10% DMSO
		Freezing method		Slow freezing
		Mycoplasma		(-)
		Animal PCR		OK
		Isozyme		LD, NP
Reference information		Reference	1
		User's Publication	4

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Reference

994  Jou TC, Takahashi M, Yasuda SI, Takaoka T.  Immunofluorescence characterization of astrocytes in monolayer culture using antiserum to glial fibrillary acidic protein.  Taiwan Yi Xue Hui Za Zhi  1983  82(11):1115-25  PubMed ID: 6368741

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User's Publication

4959  Oishi Y, Uezono Y, Yanagihara N, Izumi F, Nakamura T, Suzuki K.  Transmural compression-induced proliferation and DNA synthesis through activation of a tyrosine kinase pathway in rat astrocytoma RCR-1 cells.  Brain Res  1998  781(1-2):159-66  PubMed ID: 9507103

3930  Nemoto S, Otsuka M, Arakawa N.  Inhibitory effect of ascorbate on cell growth: relation to catalase activity.  J Nutr Sci Vitaminol (Tokyo).  1996  42(2):77-85  PubMed ID: 8780966

7049  Nemoto S, Otsuka M, Arakawa N.  A positive correlation between catalase activity and ascorbate uptake in the tissues of guinea pigs and cultured cells of mammals.  J Nutr Sci Vitaminol (Tokyo)  1996  42(5):387-95  PubMed ID: 8981246

4958  Kobayashi H, Mizuki T, Tsutsui M, Minami K, Yanagihara N, Yuhi T, Izumi F.  Receptors for C-type natriuretic peptide in cultured rat glial cells.  Brain Res  1993  617(1):163-6  PubMed ID: 8104082