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Cell No. : Cell Name
RCB0075 : RCR-1  update : 2021/01/08
CommentAstroglia cell line
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
Basic information Depositor Takaoka, Toshiko
Originator Jou,Teh-Cheng et al.
Year of deposit 1986
Animal rat < Mammals
Age at sampling 3 days
Tissue brain
Classification other
Year of origin 1979
Memo_1 Mycoplasma was eliminated with MC-210 by the depositor.
Lifespan infinite
Morphology other
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DM-160AU + 10% FBS
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Isozyme LD, NP
Reference information Reference 1
User's Publication 4


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Reference
994  Jou TC, Takahashi M, Yasuda SI, Takaoka T.  Immunofluorescence characterization of astrocytes in monolayer culture using antiserum to glial fibrillary acidic protein.  Taiwan Yi Xue Hui Za Zhi  1983  82(11):1115-25  PubMed ID: 6368741

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User's Publication
4959  Oishi Y, Uezono Y, Yanagihara N, Izumi F, Nakamura T, Suzuki K.  Transmural compression-induced proliferation and DNA synthesis through activation of a tyrosine kinase pathway in rat astrocytoma RCR-1 cells.  Brain Res  1998  781(1-2):159-66  PubMed ID: 9507103
3930  Nemoto S, Otsuka M, Arakawa N.  Inhibitory effect of ascorbate on cell growth: relation to catalase activity.  J Nutr Sci Vitaminol (Tokyo).  1996  42(2):77-85  PubMed ID: 8780966
7049  Nemoto S, Otsuka M, Arakawa N.  A positive correlation between catalase activity and ascorbate uptake in the tissues of guinea pigs and cultured cells of mammals.  J Nutr Sci Vitaminol (Tokyo)  1996  42(5):387-95  PubMed ID: 8981246
4958  Kobayashi H, Mizuki T, Tsutsui M, Minami K, Yanagihara N, Yuhi T, Izumi F.  Receptors for C-type natriuretic peptide in cultured rat glial cells.  Brain Res  1993  617(1):163-6  PubMed ID: 8104082



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