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Cell No. : Cell Name
RCB0074 : JTC-19  update : 2023/01/04
CommentRat lung fibroblast once said interferon-producing. Serum-& protein-free culturable.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Takaoka, Toshiko
Originator Takaoka, Toshiko
Year of deposit 1986
Another name RLG-1
Animal _rat < Mammals
Genus Rattus
Species norvegicus
Gender Female
Age at sampling 3 days
Tissue lung
Metastatic ability No
Classification other
Year of origin 1964
Lifespan infinite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_3632
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium DM-160AU + 2% FBS (add 10% FBS for initial cell attachment after thawing)
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Isozyme LD, NP, G6PD
Chromosome mode 37-40(50) : 37(1),38(3),39(10),40(36)
Reference information Reference 1
User's Publication 1

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147  Takaoka T, Katsuta H, Akatsuka T.  Carcinogenesis in tissue culture 24: tumorigenicity and aggregate-forming capacity of mammalian cells in culture  Jpn J Exp Med  1975  45(4):299-312  PubMed ID: 171466  

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User's Publication
4708  Ohsawa K1, Watanabe Y, Takakura A, Itoh T, Sato H.  Replication of rat coronaviruses in intestinal cell line, RCN-9, derived from F344 rats.  Exp Anim  1996  45(4):389-93  PubMed ID: 8902504   DOI: 10.1538/expanim.45.389

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