Cell No. : Cell Name
RCB0072 : RLC-27
update : 2023/01/04
|
| Comment | Liver-derived cell, tumorigenic in nude mice |
| Comment from the depositor | |
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
Takaoka, Toshiko
|
| Originator |
Takaoka, T. & Katsuta, H.
|
| Year of deposit |
1986
|
| Another name |
C27
|
| Animal |
_rat
< Mammals
|
| Gender |
Male
|
| Age at sampling |
13 days
|
| Tissue |
liver
|
| Classification |
other
|
| Year of origin |
1974
|
| Memo_1 |
Mycoplasma was eliminated with MC-210.
|
|
Lifespan |
infinite
|
| Morphology |
other
|
| Cellosaurus(Expasy) |
CVCL_3862
|
|
| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
|
Adherent cells
|
| Culture medium |
|
DM-160AU + 2% FBS (add 10% FBS for initial cell attachment after thawing)
|
| Antibiotics |
|
Free
|
|
Passage method |
|
0.25% Trypsin
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Culture information
|
Passage ratio |
|
1 : 8 split
|
| SC frequency |
|
Subculture : 2 times/week
|
| Temperature |
|
37
℃
|
| CO2 concentration |
|
5
%
|
| Freeze medium |
|
Medium + 10% DMSO
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| Freezing method |
|
Slow freezing
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| Mycoplasma/Acholeplasma |
|
(-)
|
| Animal PCR |
|
OK
|
| Isozyme |
|
LD, NP
|
| Reference information |
Reference |
1
|
| User's Publication |
0
|
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